[NiFe] Group 4f-hydrogenase Putative

This entry was last updated at: June 13, 2016, 11:25 a.m.

Properties
Group [NiFe] Group 4: Respiratory H2-evolving [NiFe] hydrogenases
Subgroup [NiFe] Group 4f: Formate-coupled
Function Unconfirmed role. May form respiratory complex that couples oxidation of formate to proton reduction. The energy generated is likely to be used to translocate protons through the antiporter-like subunits.
Activity H2-evolution?
Oxygen tolerance O2-sensitive
Localisation Membrane-bound
Distribution
Ecosystem distribution In low abundance in anoxic soils
Taxonomic distribution Encoded in several classes of Firmicutes
Genetic Organisation

e.g. Desulfosporosinus orientis (WP_014183752.1):

Architecture
Structures
    None
Subunits 6?
Subunit description EhfE (hydrogenase large subunit)
EhfF (hydrogenase small subunit)
EhfB (NuoH-like transmembrane adaptor)
EhfA (NuoL-like transmembrane adaptor)
EhfCD (antiporter-like subunits)
Catalytic site [NiFe]-centre
FeS clusters Proximal: 4Cys[4Fe4S]
Medial: Absent
Distal: Absent
Important Notes
This hydrogenase class has only been identified based on genomic predictions. Physiological, genetic, and biochemical studies are needed to confirm its electron donor and describe its properties.
Sequences in this class
NCBI AccessionOrganismHydrogenase ClassPhylumOrderActivity (Predicted)Oxygen Tolerance (Predicted)Subunits (Predicted)Metal Centres (Predicted)Accessory Subunits (Predicted)
WP_004094701.1Acetonema longum[NiFe] Group 4fFirmicutesSelenomadalesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, two antiporter subunits
WP_027938372.1Anaeroarcus burkinensis[NiFe] Group 4fFirmicutesSelenomadalesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, two antiporter subunits
WP_018702624.1Anaeromusa acidaminophila[NiFe] Group 4fFirmicutesSelenomadalesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, two antiporter subunits
WP_027397032.1Anaerovibrio lipolyticus[NiFe] Group 4fFirmicutesSelenomadalesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, two antiporter subunits
WP_027406274.1Anaerovibrio sp. RM50[NiFe] Group 4fFirmicutesSelenomadalesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, two antiporter subunits
WP_021718870.1Phascolarctobacterium sp. CAG:207[NiFe] Group 4fFirmicutesSelenomadalesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, two antiporter subunits
WP_021167282.1Sporomusa ovata[NiFe] Group 4fFirmicutesSelenomadalesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, two antiporter subunits
WP_019880045.1Succinispira mobilis[NiFe] Group 4fFirmicutesSelenomadalesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, two antiporter subunits
WP_019553945.1Zymophilus raffinosivorans[NiFe] Group 4fFirmicutesSelenomadalesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, two antiporter subunits
WP_005582638.1Clostridium ultunense[NiFe] Group 4fFirmicutesClostridialesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, two antiporter subunits
WP_014795396.1Desulfitobacterium dehalogenans[NiFe] Group 4fFirmicutesClostridialesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, two antiporter subunits
WP_015945149.1Desulfitobacterium hafniense[NiFe] Group 4fFirmicutesClostridialesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, two antiporter subunits
WP_019851221.1Desulfitobacterium sp. PCE1[NiFe] Group 4fFirmicutesClostridialesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, two antiporter subunits
WP_014902103.1Desulfosporosinus meridiei[NiFe] Group 4fFirmicutesClostridialesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, two antiporter subunits
WP_014183752.1Desulfosporosinus orientis[NiFe] Group 4fFirmicutesClostridialesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, two antiporter subunits
WP_009619272.1Desulfosporosinus sp. OT[NiFe] Group 4fFirmicutesClostridialesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, two antiporter subunits
WP_007780611.1Desulfosporosinus youngiae[NiFe] Group 4fFirmicutesClostridialesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, two antiporter subunits
WP_015756422.1Desulfotomaculum acetoxidans[NiFe] Group 4fFirmicutesClostridialesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, two antiporter subunits
WP_013843216.1Desulfotomaculum ruminis[NiFe] Group 4fFirmicutesClostridialesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, two antiporter subunits
WP_013623369.1Syntrophobotulus glycolicus[NiFe] Group 4fFirmicutesClostridialesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, two antiporter subunits
WP_018133527.1Alicyclobacillus pohliae[NiFe] Group 4fFirmicutesBacillalesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, two antiporter subunits
WP_006323220.1Anoxybacillus flavithermus[NiFe] Group 4fFirmicutesBacillalesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, two antiporter subunits
WP_028986298.1Thermicanus aegyptius[NiFe] Group 4fFirmicutesBacillalesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, two antiporter subunits

Literature

Genetics: