This entry was last updated at: June 13, 2016, 11:25 a.m.
Group | [NiFe] Group 4: Respiratory H2-evolving [NiFe] hydrogenases |
Subgroup | [NiFe] Group 4f: Formate-coupled |
Function | Unconfirmed role. May form respiratory complex that couples oxidation of formate to proton reduction. The energy generated is likely to be used to translocate protons through the antiporter-like subunits. |
Activity | H2-evolution? |
Oxygen tolerance | O2-sensitive |
Localisation | Membrane-bound |
Ecosystem distribution | In low abundance in anoxic soils |
Taxonomic distribution | Encoded in several classes of Firmicutes |
e.g. Desulfosporosinus orientis (WP_014183752.1):
Structures |
|
Subunits | 6? |
Subunit description | EhfE (hydrogenase large subunit) EhfF (hydrogenase small subunit) EhfB (NuoH-like transmembrane adaptor) EhfA (NuoL-like transmembrane adaptor) EhfCD (antiporter-like subunits) |
Catalytic site | [NiFe]-centre |
FeS clusters | Proximal: 4Cys[4Fe4S] Medial: Absent Distal: Absent |
NCBI Accession | Organism | Hydrogenase Class | Phylum | Order | Activity (Predicted) | Oxygen Tolerance (Predicted) | Subunits (Predicted) | Metal Centres (Predicted) | Accessory Subunits (Predicted) |
---|---|---|---|---|---|---|---|---|---|
WP_004094701.1 | Acetonema longum | [NiFe] Group 4f | Firmicutes | Selenomadales | Evolving | Sensitive | 6 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two antiporter subunits |
WP_027938372.1 | Anaeroarcus burkinensis | [NiFe] Group 4f | Firmicutes | Selenomadales | Evolving | Sensitive | 6 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two antiporter subunits |
WP_018702624.1 | Anaeromusa acidaminophila | [NiFe] Group 4f | Firmicutes | Selenomadales | Evolving | Sensitive | 6 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two antiporter subunits |
WP_027397032.1 | Anaerovibrio lipolyticus | [NiFe] Group 4f | Firmicutes | Selenomadales | Evolving | Sensitive | 6 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two antiporter subunits |
WP_027406274.1 | Anaerovibrio sp. RM50 | [NiFe] Group 4f | Firmicutes | Selenomadales | Evolving | Sensitive | 6 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two antiporter subunits |
WP_021718870.1 | Phascolarctobacterium sp. CAG:207 | [NiFe] Group 4f | Firmicutes | Selenomadales | Evolving | Sensitive | 6 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two antiporter subunits |
WP_021167282.1 | Sporomusa ovata | [NiFe] Group 4f | Firmicutes | Selenomadales | Evolving | Sensitive | 6 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two antiporter subunits |
WP_019880045.1 | Succinispira mobilis | [NiFe] Group 4f | Firmicutes | Selenomadales | Evolving | Sensitive | 6 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two antiporter subunits |
WP_019553945.1 | Zymophilus raffinosivorans | [NiFe] Group 4f | Firmicutes | Selenomadales | Evolving | Sensitive | 6 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two antiporter subunits |
WP_005582638.1 | Clostridium ultunense | [NiFe] Group 4f | Firmicutes | Clostridiales | Evolving | Sensitive | 6 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two antiporter subunits |
WP_014795396.1 | Desulfitobacterium dehalogenans | [NiFe] Group 4f | Firmicutes | Clostridiales | Evolving | Sensitive | 6 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two antiporter subunits |
WP_015945149.1 | Desulfitobacterium hafniense | [NiFe] Group 4f | Firmicutes | Clostridiales | Evolving | Sensitive | 6 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two antiporter subunits |
WP_019851221.1 | Desulfitobacterium sp. PCE1 | [NiFe] Group 4f | Firmicutes | Clostridiales | Evolving | Sensitive | 6 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two antiporter subunits |
WP_014902103.1 | Desulfosporosinus meridiei | [NiFe] Group 4f | Firmicutes | Clostridiales | Evolving | Sensitive | 6 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two antiporter subunits |
WP_014183752.1 | Desulfosporosinus orientis | [NiFe] Group 4f | Firmicutes | Clostridiales | Evolving | Sensitive | 6 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two antiporter subunits |
WP_009619272.1 | Desulfosporosinus sp. OT | [NiFe] Group 4f | Firmicutes | Clostridiales | Evolving | Sensitive | 6 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two antiporter subunits |
WP_007780611.1 | Desulfosporosinus youngiae | [NiFe] Group 4f | Firmicutes | Clostridiales | Evolving | Sensitive | 6 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two antiporter subunits |
WP_015756422.1 | Desulfotomaculum acetoxidans | [NiFe] Group 4f | Firmicutes | Clostridiales | Evolving | Sensitive | 6 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two antiporter subunits |
WP_013843216.1 | Desulfotomaculum ruminis | [NiFe] Group 4f | Firmicutes | Clostridiales | Evolving | Sensitive | 6 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two antiporter subunits |
WP_013623369.1 | Syntrophobotulus glycolicus | [NiFe] Group 4f | Firmicutes | Clostridiales | Evolving | Sensitive | 6 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two antiporter subunits |
WP_018133527.1 | Alicyclobacillus pohliae | [NiFe] Group 4f | Firmicutes | Bacillales | Evolving | Sensitive | 6 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two antiporter subunits |
WP_006323220.1 | Anoxybacillus flavithermus | [NiFe] Group 4f | Firmicutes | Bacillales | Evolving | Sensitive | 6 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two antiporter subunits |
WP_028986298.1 | Thermicanus aegyptius | [NiFe] Group 4f | Firmicutes | Bacillales | Evolving | Sensitive | 6 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two antiporter subunits |
Genetics: