[NiFe] Group 1g-hydrogenase
This entry was last updated at: June 13, 2016, 11:11 a.m.
Properties
| Group | [NiFe] Group 1: Membrane-bound H2-uptake [NiFe] hydrogenases |
| Subgroup | [NiFe] Group 1g: Crenarchaeota-type |
| Function | Hydrogenotrophic respiration using sulfur as terminal electron acceptor. Route of electron transfer unresolved. |
| Activity | H2-uptake (unidirectional) |
| Oxygen tolerance | O2-sensitive? |
| Localisation | Membrane-bound |
Distribution
| Ecosystem distribution | Associated with high-temperature environments such as hydrothermal systems |
| Taxonomic distribution | Exclusive to Crenarchaeota |
Genetic Organisation
e.g. Thermoproteus neutrophilus (WP_012349775.1):
Architecture
| Structures |
|
| Subunits | 4? |
| Subunit description | None |
| Catalytic site | [NiFe]-centre |
| FeS clusters | Proximal: 3Cys1Asp[4Fe4S] or 3Cys1Asn[4Fe4S] Medial: 4Cys[4Fe4S] Distal: 3Cys1His[4Fe4S] or 3Cys1Arg[4Fe4S] |
Sequences in this class
| NCBI Accession | Organism | Hydrogenase Class | Phylum | Order | Activity (Predicted) | Oxygen Tolerance (Predicted) | Subunits (Predicted) | Metal Centres (Predicted) | Accessory Subunits (Predicted) |
|---|---|---|---|---|---|---|---|---|---|
| WP_011822511.1 | Hyperthermus butylicus | [NiFe] Group 1g | Crenarchaeota | Desulfurococcales | Anaerobic Uptake | Unknown | 3 | [NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster | Cytochrome b |
| WP_012123507.1 | Ignicoccus hospitalis | [NiFe] Group 1g | Crenarchaeota | Desulfurococcales | Anaerobic Uptake | Unknown | 4 | [NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster | Cytochrome b, [FeS] protein |
| WP_014025627.1 | Pyrolobus fumarii | [NiFe] Group 1g | Crenarchaeota | Desulfurococcales | Anaerobic Uptake | Unknown | 4 | [NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster | Cytochrome b, [FeS] protein |
| WP_011900768.1 | Pyrobaculum arsenaticum | [NiFe] Group 1g | Crenarchaeota | Thermoproteales | Anaerobic Uptake | Unknown | 4 | [NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster | Cytochrome b, [FeS] protein |
| WP_011850254.1 | Pyrobaculum calidifontis | [NiFe] Group 1g | Crenarchaeota | Thermoproteales | Anaerobic Uptake | Unknown | 4 | [NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster | Cytochrome b, [FeS] protein |
| WP_011761956.1 | Pyrobaculum islandicum | [NiFe] Group 1g | Crenarchaeota | Thermoproteales | Anaerobic Uptake | Unknown | 4 | [NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster | Cytochrome b, [FeS] protein |
| WP_014346469.1 | Pyrobaculum oguniense | [NiFe] Group 1g | Crenarchaeota | Thermoproteales | Anaerobic Uptake | Unknown | 4 | [NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster | Cytochrome b, [FeS] protein |
| WP_011752262.1 | Thermofilum pendens | [NiFe] Group 1g | Crenarchaeota | Thermoproteales | Anaerobic Uptake | Unknown | 4 | [NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster | Cytochrome b, [FeS] protein |
| WP_020962524.1 | Thermofilum sp. 1910b | [NiFe] Group 1g | Crenarchaeota | Thermoproteales | Anaerobic Uptake | Unknown | 4 | [NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster | Cytochrome b, [FeS] protein |
| WP_012349775.1 | Thermoproteus neutrophilus | [NiFe] Group 1g | Crenarchaeota | Thermoproteales | Anaerobic Uptake | Unknown | 4 | [NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster | Cytochrome b, [FeS] protein |
| WP_014125970.1 | Thermoproteus tenax | [NiFe] Group 1g | Crenarchaeota | Thermoproteales | Anaerobic Uptake | Unknown | 4 | [NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster | Cytochrome b, [FeS] protein |
| WP_013680997.1 | Thermoproteus uzoniensis | [NiFe] Group 1g | Crenarchaeota | Thermoproteales | Anaerobic Uptake | Unknown | 4 | [NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster | Cytochrome b, [FeS] protein |
| WP_013337401.1 | Vulcanisaeta distributa | [NiFe] Group 1g | Crenarchaeota | Thermoproteales | Anaerobic Uptake | Unknown | 4 | [NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster | Cytochrome b, [FeS] protein |
| WP_048100721.1 | Acidianus copahuensis | [NiFe] Group 1g | Crenarchaeota | Sulfolobales | Anaerobic Uptake | Unknown | 4 | [NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster | Cytochrome b, [FeS] protein |
| WP_048100713.1 | Acidianus copahuensis | [NiFe] Group 1g | Crenarchaeota | Sulfolobales | Anaerobic Uptake | Unknown | 4 | [NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster | Cytochrome b, [FeS] protein |
Literature
Genetics:
- Greening, C., Biswas, A., Carere, C.R., Jackson, C.J., Taylor, M.C., Stott, M.B., Cook, G.M., Morales, S.E. (2016) Genomic and metagenomic surveys of hydrogenase distribution indicate H2 is a widely utilised energy source for microbial growth and survival. ISME J. 10: 761-777.
Physiology:
- Brock, T.D., Brock, K.M., Belly, R.T., and Weiss, R.L. (1972) Sulfolobus: a new genus of sulfur-oxidizing bacteria living at low pH and high temperature. Arch. Microbiol. 84: 54-68.
- Fischer, F., Zillig, W., Stetter, K.O., and Schreiber, G. (1983) Chemolithoautotrophic metabolism of anaerobic extremely thermophilic archaebacteria. Nature 301: 511-513.
- Huber, H., Burggraf, S., Mayer, T., Wyschkony, I., Rachel, R., and Stetter, K.O. (2000). Ignicoccus gen. nov., a novel genus of hyperthermophilic, chemolithoautotrophic Archaea, represented by two new species, Ignicoccus islandicus sp. nov. and Ignicoccus pacificus sp. nov. Int. J. Syst. Evol. Microbiol. 50: 2093-2100.
- Laska, S., Lottspeich, F., and Kletzin, A. (2003) Membrane-bound hydrogenase and sulfur reductase of the hyperthermophilic and acidophilic archaeon Acidianus ambivalens. Microbiol. 149: 2357-2371.
- Pihl, T.D., Schicho, R.N., Kelly, R.M., and Maier, R.J. (1989) Characterization of hydrogen-uptake activity in the hyperthermophile Pyrodictium brockii. Proc. Natl. Acad. Sci. U.S.A. 86: 138-141.
- Segerer, A., Neuner, A., Kristjansson, J.K., and Stetter, K.O. (1986) Acidianus infernus gen. nov., sp. nov., and Acidianus brierleyi comb. nov. — facultatively aerobic, extremely acidophilic thermophilic sulfur-metabolizing archaebacteria. Int. J. Syst. Bacteriol. 36: 559-564.
- Stetter, K.O., Helmut, K., and Stackebrandt, E. (1983) Pyrodictium gen. nov., a new genus of submarine disc-shaped sulphur reducing archaebacteria growing optimally at 105 °C.