[NiFe] Group 1f-hydrogenase

This entry was last updated at: Feb. 9, 2018, 7:32 a.m.

Properties
Group [NiFe] Group 1: Respiratory H2-uptake [NiFe] hydrogenases
Subgroup [NiFe] Group 1f: Oxygen-protecting
Function Unresolved role. Some enzymes mediate hydrogenotrophic respiration using O2 as a terminal electron acceptor. Enzyme also linked to protection from reactive oxygen species, protection of nitrogenase protection, and recycling of nitrogenase-derived H2.
Activity H2-uptake (unidirectional)?
Oxygen tolerance O2-tolerant?
Localisation Membrane-bound
Distribution
Ecosystem distribution Various soil and aquatic environments
Taxonomic distribution Present in both aerobic soil bacteria (e.g. Actinobacteria) and anaerobic marine bacteria (e.g. Deltaproteobacteria)
Genetic Organisation

e.g. Geobacter sulfurreducens (WP_010940798.1):

Architecture
Structures
    None
Subunits 3
Subunit description HyoL (hydrogenase large subunit)
HyoS (hydrogenase small subunit)
HyoE (cytochrome b subunit)
Catalytic site [NiFe]-centre
FeS clusters Proximal: 3Cys1Asp[4Fe4S] or 3Cys1Asn[4Fe4S]
Medial: 3Cys[3Fe4S]
Distal: 3Cys1His[4Fe4S]
Important Notes
This hydrogenase class has been minimally studied. Further genetic, physiological, and biochemical studies are required to understand its role and behaviour. There is some phylogenetic evidence that this class is polyphyletic and may in fact include several loosely-related hydrogenases with distinct roles.
Sequences in this class
NCBI AccessionOrganismHydrogenase ClassPhylumOrderActivity (Predicted)Oxygen Tolerance (Predicted)Subunits (Predicted)Metal Centres (Predicted)Accessory Subunits (Predicted)
WP_022865058.1Varibaculum cambriense[NiFe] Group 1fActinobacteriaActinomycetalesAnaerobic UptakeUnknown3[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] clusterCytochrome b
WP_012546172.1Thermodesulfovibrio yellowstonii[NiFe] Group 1fNitrospiraeNitrospiralesAnaerobic UptakeUnknown3[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] clusterCytochrome b
WP_028845527.1Thermodesulfovibrio thiophilus[NiFe] Group 1fNitrospiraeNitrospiralesAnaerobic UptakeUnknown3[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] clusterCytochrome b
WP_028842875.1Thermodesulfovibrio islandicus[NiFe] Group 1fNitrospiraeNitrospiralesAnaerobic UptakeUnknown3[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] clusterCytochrome b
WP_011957045.1Roseiflexus sp. RS-1[NiFe] Group 1fChloroflexiChloroflexalesAnaerobic UptakeUnknown3[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] clusterCytochrome b
WP_012121625.1Roseiflexus castenholzii[NiFe] Group 1fChloroflexiChloroflexalesAnaerobic UptakeUnknown3[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] clusterCytochrome b
WP_006562194.1Oscillochloris trichoides[NiFe] Group 1fChloroflexiChloroflexalesAnaerobic UptakeUnknown3[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] clusterCytochrome b
WP_004013385.1Mobiluncus mulieris[NiFe] Group 1fActinobacteriaActinomycetalesAnaerobic UptakeUnknown3[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] clusterCytochrome b
WP_004007712.1Mobiluncus curtisii[NiFe] Group 1fActinobacteriaActinomycetalesAnaerobic UptakeUnknown3[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] clusterCytochrome b
WP_022851572.1Geovibrio sp. L21-Ace-BES[NiFe] Group 1fDeferribacteresDeferribacteralesAnaerobic UptakeUnknown3[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] clusterCytochrome b
WP_020678214.1Geopsychrobacter electrodiphilus[NiFe] Group 1fDeltaproteobacteriaDesulfuromonadalesAnaerobic UptakeUnknown3[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] clusterCytochrome b
WP_011937483.1Geobacter uraniireducens[NiFe] Group 1fDeltaproteobacteriaDesulfuromonadalesAnaerobic UptakeUnknown3[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] clusterCytochrome b
WP_010940798.1Geobacter sulfurreducens[NiFe] Group 1fDeltaproteobacteriaDesulfuromonadalesAnaerobic UptakeUnknown3[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] clusterCytochrome b
WP_015718812.1Geobacter sp. M18[NiFe] Group 1fDeltaproteobacteriaDesulfuromonadalesAnaerobic UptakeUnknown3[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] clusterCytochrome b
WP_004512555.1Geobacter metallireducens[NiFe] Group 1fDeltaproteobacteriaDesulfuromonadalesAnaerobic UptakeUnknown3[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] clusterCytochrome b
WP_012470110.1Geobacter lovleyi[NiFe] Group 1fDeltaproteobacteriaDesulfuromonadalesAnaerobic UptakeUnknown3[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] clusterCytochrome b
WP_026840074.1Geobacter bremensis[NiFe] Group 1fDeltaproteobacteriaDesulfuromonadalesAnaerobic UptakeUnknown3[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] clusterCytochrome b
WP_012529798.1Geobacter bemidjiensis[NiFe] Group 1fDeltaproteobacteriaDesulfuromonadalesAnaerobic UptakeUnknown3[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] clusterCytochrome b
WP_006544185.1Frankia sp. EUN1f[NiFe] Group 1fActinobacteriaActinomycetalesAnaerobic UptakeUnknown3[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] clusterCytochrome b
WP_020460947.1Frankia sp. EAN1pec[NiFe] Group 1fActinobacteriaActinomycetalesAnaerobic UptakeUnknown3[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] clusterCytochrome b
WP_018637732.1Frankia sp. BMG5.12[NiFe] Group 1fActinobacteriaActinomycetalesAnaerobic UptakeUnknown3[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] clusterCytochrome b
WP_018502767.1Frankia sp. BCU110501[NiFe] Group 1fActinobacteriaActinomycetalesAnaerobic UptakeUnknown3[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] clusterCytochrome b
WP_013885838.1Flexistipes sinusarabici[NiFe] Group 1fDeferribacteresDeferribacteralesAnaerobic UptakeUnknown3[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] clusterCytochrome b
WP_027713781.1Desulfuromonas sp. TF[NiFe] Group 1fDeltaproteobacteriaDesulfuromonadalesAnaerobic UptakeUnknown3[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] clusterCytochrome b
WP_013011747.1Denitrovibrio acetiphilus[NiFe] Group 1fDeferribacteresDeferribacteralesAnaerobic UptakeUnknown3[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] clusterCytochrome b
WP_020489980.1Dehalobacter sp. FTH1[NiFe] Group 1fFirmicutesClostridialesAnaerobic UptakeUnknown3[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] clusterCytochrome b
WP_025324524.1Deferrisoma camini[NiFe] Group 1fDeltaproteobacteriaUnclassifiedAnaerobic UptakeUnknown3[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] clusterCytochrome b
WP_013006836.1Deferribacter desulfuricans[NiFe] Group 1fDeferribacteresDeferribacteralesAnaerobic UptakeUnknown3[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] clusterCytochrome b
WP_025252525.1Corynebacterium vitaeruminis[NiFe] Group 1fActinobacteriaActinomycetalesAnaerobic UptakeUnknown3[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] clusterCytochrome b
WP_016422186.1Corynebacterium sp. HFH0082[NiFe] Group 1fActinobacteriaActinomycetalesAnaerobic UptakeUnknown3[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] clusterCytochrome b
WP_016458088.1 Corynebacterium pyruviciproducens[NiFe] Group 1fActinobacteriaActinomycetalesAnaerobic UptakeUnknown3[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] clusterCytochrome b
WP_005388873.1Corynebacterium glucuronolyticum[NiFe] Group 1fActinobacteriaActinomycetalesAnaerobic UptakeUnknown3[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] clusterCytochrome b
WP_027012481.1Corynebacterium freiburgense[NiFe] Group 1fActinobacteriaActinomycetalesAnaerobic UptakeUnknown3[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] clusterCytochrome b
WP_006062526.1Corynebacterium durum[NiFe] Group 1fActinobacteriaActinomycetalesAnaerobic UptakeUnknown3[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] clusterCytochrome b
WP_014310124.1Corynebacterium diphtheriae[NiFe] Group 1fActinobacteriaActinomycetalesAnaerobic UptakeUnknown3[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] clusterCytochrome b
WP_005509950.1Corynebacterium amycolatum[NiFe] Group 1fActinobacteriaActinomycetalesAnaerobic UptakeUnknown3[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] clusterCytochrome b
WP_013450229.1Calditerrivibrio nitroreducens[NiFe] Group 1fDeferribacteresDeferribacteralesAnaerobic UptakeUnknown3[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] clusterCytochrome b
WP_022397361.1Akkermansia sp. CAG:344[NiFe] Group 1fVerrucomicrobiaVerrucomicrobialesAnaerobic UptakeUnknown3[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] clusterCytochrome b
WP_012421152.1Akkermansia muciniphila[NiFe] Group 1fVerrucomicrobiaVerrucomicrobialesAnaerobic UptakeUnknown3[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] clusterCytochrome b
WP_026441619.1Acidobacterium sp. PMMR2[NiFe] Group 1fAcidobacteriaAcidobacterialesAnaerobic UptakeUnknown3[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] clusterCytochrome b
WP_015898101.1Acidobacterium capsulatum[NiFe] Group 1fAcidobacteriaAcidobacterialesAnaerobic UptakeUnknown3[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] clusterCytochrome b

Literature

Genetics:

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