[FeFe] Group C2-hydrogenase Putative
This entry was last updated at: Feb. 9, 2018, 6:15 a.m.
Properties
| Group | [FeFe]-hydrogenases |
| Subgroup | [FeFe] Group C2: Chemotactic |
| Function | Unconfirmed role. May serve as H2-sensors in regulatory cascades. Enzyme predicted to transfer H2-liberated electrons to PAS domain. Associated redox change may modulate activity of associated methyl-accepting chemotaxis proteins or diguanylate cyclases in turn leading to regulation of cellular motility. |
| Activity | H2-sensing? |
| Oxygen tolerance | O2-labile |
| Localisation | Cytosolic |
Distribution
| Ecosystem distribution | Gastrointestinal tracts, anoxic soils and sediments |
| Taxonomic distribution | Encoded in anaerobic bacteria, including Firmicutes and Spirochaetes |
Genetic Organisation
Organisation 1: Clostridium lundense (WP_027626440.1):
Organisation 2: Desulfosporosinus orientis (WP_014186347.1):
Architecture
| Structures |
|
| Subunits | 1 |
| Subunit description | Organisation 1: HydS fusion (catalytic subunit with PAS domain fused to methyl-accepting chemotaxis protein domain) Organisation 2: HydS fusion (catalytic subunit with PAS domain fused to diguanylate cyclase domain) |
| Catalytic site | [FeFe]-centre |
| FeS clusters | 2 [4Fe4S] clusters |
Sequences in this class
| NCBI Accession | Organism | Hydrogenase Class | Phylum | Order | Activity (Predicted) | Oxygen Tolerance (Predicted) | Subunits (Predicted) | Metal Centres (Predicted) | Accessory Subunits (Predicted) |
|---|---|---|---|---|---|---|---|---|---|
| WP_009618950.1 | Desulfosporosinus sp. OT | [FeFe] Group C2 | Firmicutes | Clostridiales | Sensory | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters, PAS domain | Fusion with diguanylate cyclase |
| WP_014186347.1 | Desulfosporosinus orientis | [FeFe] Group C2 | Firmicutes | Clostridiales | Sensory | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters, PAS domain | Fusion with diguanylate cyclase |
| WP_011640458.1 | Syntrophomonas wolfei | [FeFe] Group C2 | Firmicutes | Clostridiales | Sensory | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters, PAS domain | Fusion with histidine kinase |
| WP_014186754.1 | Desulfosporosinus orientis | [FeFe] Group C2 | Firmicutes | Clostridiales | Sensory | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters, PAS domain | Fusion with histidine kinase |
| WP_015709814.1 | Treponema azotonutricium | [FeFe] Group C2 | Spirochaetes | Spirochaetales | Sensory | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters, PAS domain | Fusion with histidine kinase |
| WP_002848484.1 | Ruminococcus albus | [FeFe] Group C2 | Firmicutes | Clostridiales | Sensory | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters, PAS domain | Fusion with methyl-accepting chemotaxis protein |
| WP_004064152.1 | Eubacterium plexicaudatum | [FeFe] Group C2 | Firmicutes | Clostridiales | Sensory | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters, PAS domain | Fusion with methyl-accepting chemotaxis protein |
| WP_010251391.1 | Acetivibrio cellulolyticus | [FeFe] Group C2 | Firmicutes | Clostridiales | Sensory | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters, PAS domain | Fusion with methyl-accepting chemotaxis protein |
| WP_013255981.1 | Spirochaeta smaragdinae | [FeFe] Group C2 | Spirochaetes | Spirochaetales | Sensory | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters, PAS domain | Fusion with methyl-accepting chemotaxis protein |
| WP_013314414.1 | Spirochaeta thermophila | [FeFe] Group C2 | Spirochaetes | Spirochaetales | Sensory | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters, PAS domain | Fusion with methyl-accepting chemotaxis protein |
| WP_013623612.1 | Syntrophobotulus glycolicus | [FeFe] Group C2 | Firmicutes | Clostridiales | Sensory | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters, PAS domain | Fusion with methyl-accepting chemotaxis protein |
| WP_013701174.1 | Treponema succinifaciens | [FeFe] Group C2 | Spirochaetes | Spirochaetales | Sensory | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters, PAS domain | Fusion with methyl-accepting chemotaxis protein |
| WP_013757426.1 | Treponema brennaborense | [FeFe] Group C2 | Spirochaetes | Spirochaetales | Sensory | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters, PAS domain | Fusion with methyl-accepting chemotaxis protein |
| WP_013907653.1 | Thermodesulfatator indicus | [FeFe] Group C2 | Thermodesulfobacteria | Thermodesulfobacteriales | Sensory | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters, PAS domain | Fusion with methyl-accepting chemotaxis protein |
| WP_013969799.1 | Treponema caldaria | [FeFe] Group C2 | Spirochaetes | Spirochaetales | Sensory | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters, PAS domain | Fusion with methyl-accepting chemotaxis protein |
| WP_014080084.1 | Roseburia hominis | [FeFe] Group C2 | Firmicutes | Clostridiales | Sensory | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters, PAS domain | Fusion with methyl-accepting chemotaxis protein |
| WP_014184511.1 | Desulfosporosinus orientis | [FeFe] Group C2 | Firmicutes | Clostridiales | Sensory | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters, PAS domain | Fusion with methyl-accepting chemotaxis protein |
| WP_014903221.1 | Desulfosporosinus meridiei | [FeFe] Group C2 | Firmicutes | Clostridiales | Sensory | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters, PAS domain | Fusion with methyl-accepting chemotaxis protein |
| WP_015560495.1 | Roseburia intestinalis | [FeFe] Group C2 | Firmicutes | Clostridiales | Sensory | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters, PAS domain | Fusion with methyl-accepting chemotaxis protein |
| WP_015713064.1 | Treponema azotonutricium | [FeFe] Group C2 | Spirochaetes | Spirochaetales | Sensory | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters, PAS domain | Fusion with methyl-accepting chemotaxis protein |
| WP_022931679.1 | Treponema bryantii | [FeFe] Group C2 | Spirochaetes | Spirochaetales | Sensory | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters, PAS domain | Fusion with methyl-accepting chemotaxis protein |
| WP_027398671.1 | Anaerovorax odorimutans | [FeFe] Group C2 | Firmicutes | Clostridiales | Sensory | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters, PAS domain | Fusion with methyl-accepting chemotaxis protein |
| WP_027626440.1 | Clostridium lundense | [FeFe] Group C2 | Firmicutes | Clostridiales | Sensory | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters, PAS domain | Fusion with methyl-accepting chemotaxis protein |
| WP_028517519.1 | Ruminococcus flavefaciens | [FeFe] Group C2 | Firmicutes | Clostridiales | Sensory | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters, PAS domain | Fusion with methyl-accepting chemotaxis protein |
| WP_007957068.1 | Pelosinus fermentans | [FeFe] Group C2 | Firmicutes | Selenomonadales | Sensory | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters, PAS domain | None |
Literature
Genetics:
- Greening, C., Biswas, A., Carere, C.R., Jackson, C.J., Taylor, M.C., Stott, M.B., Cook, G.M., and Morales, S.E. (2016) Genomic and metagenomic surveys of hydrogenase distribution indicate H2 is a widely utilised energy source for microbial growth and survival. ISME J. 10: 761-777.
- Wolf, P.G., Biswas, A., Morales, S.E., Greening, C., and Gaskins, H.R. (2016) H2 metabolism is widespread and diverse among human colonic microbes. Gut Microb. 7: 235-245.
- Søndergaard, D., Petersen, C.N.S., and Greening, C. (2017) HydDB: a web tool for hydrogenase classification and analysis. Sci. Rep. 6: 34212.