[FeFe] Group B-hydrogenase Putative
This entry was last updated at: Feb. 9, 2018, 6:17 a.m.
Properties
| Group | [FeFe]-hydrogenases |
| Subgroup | [FeFe] Group B: Colonic-type |
| Function | Unconfirmed role. Indirect evidence suggests the hydrogenase is involved in hydrogenogenic fermentation. It may couple reoxidation of ferredoxinred to the evolution of H2. |
| Activity | H2-evolution? |
| Oxygen tolerance | O2-labile |
| Localisation | Cytosolic |
Distribution
| Ecosystem distribution | Gastrointestinal tracts, anoxic soils and sediments |
| Taxonomic distribution | Widespread in colonic Bacteroides, Clostridia, and Actinobacteria |
Genetic Organisation
Organisation 1: Clostridium pasteurianum (WP_023976023.1):
Organisation 2: Clostridium carboxyidovorans (WP_007061531.1):
Architecture
| Structures |
|
| Subunits | 1 |
| Subunit description | HydM (catalytic subunit) |
| Catalytic site | [FeFe]-centre |
| FeS clusters | Organisation 1: 2 [4Fe4S] clusters Organisation 2: 3 [4Fe4S] clusters, 1 [2Fe2S] cluste |
Sequences in this class
| NCBI Accession | Organism | Hydrogenase Class | Phylum | Order | Activity (Predicted) | Oxygen Tolerance (Predicted) | Subunits (Predicted) | Metal Centres (Predicted) | Accessory Subunits (Predicted) |
|---|---|---|---|---|---|---|---|---|---|
| WP_019554692.1 | Zymophilus raffinosivorans | [FeFe] Group B | Firmicutes | Selenomonadales | Evolving | Labile | 1 | [FeFe]-centre | None |
| WP_023386490.1 | Youngiibacter fragilis | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_004611414.1 | Tyzzerella nexilis | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_004613839.1 | Tyzzerella nexilis | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_013700376.1 | Treponema succinifaciens | [FeFe] Group B | Spirochaetes | Spirochaetales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_002704855.1 | Treponema saccharophilum | [FeFe] Group B | Spirochaetes | Spirochaetales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_010699218.1 | Treponema denticola | [FeFe] Group B | Spirochaetes | Spirochaetales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_022931411.1 | Treponema bryantii | [FeFe] Group B | Spirochaetes | Spirochaetales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_015710040.1 | Treponema azotonutricium | [FeFe] Group B | Spirochaetes | Spirochaetales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_007289794.1 | Thermosinus carboxydivorans | [FeFe] Group B | Firmicutes | Selenomonadales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_007290224.1 | Thermosinus carboxydivorans | [FeFe] Group B | Firmicutes | Selenomonadales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_013276251.1 | Thermosediminibacter oceani | [FeFe] Group B | Firmicutes | Thermoanaerobacterales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_013788347.1 | Thermoanaerobacterium xylanolyticum | [FeFe] Group B | Firmicutes | Thermoanaerobacterales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_013298245.1 | Thermoanaerobacterium thermosaccharolyticum | [FeFe] Group B | Firmicutes | Thermoanaerobacterales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_014758430.1 | Thermoanaerobacterium saccharolyticum | [FeFe] Group B | Firmicutes | Thermoanaerobacterales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_014063301.1 | Thermoanaerobacter wiegelii | [FeFe] Group B | Firmicutes | Thermoanaerobacterales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | Phosphatase |
| WP_013150225.1 | Thermoanaerobacter mathranii | [FeFe] Group B | Firmicutes | Thermoanaerobacterales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_008713427.1 | Synergistes sp. 3_1_syn1 | [FeFe] Group B | Synergistetes | Synergistales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_019880475.1 | Succinispira mobilis | [FeFe] Group B | Firmicutes | Selenomonadales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_007046798.1 | Subdoligranulum variabile | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_028044437.1 | Stoquefichus massiliensis | [FeFe] Group B | Firmicutes | Erysipelotrichales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_021170205.1 | Sporomusa ovata | [FeFe] Group B | Firmicutes | Selenomonadales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_013255832.1 | Spirochaeta smaragdinae | [FeFe] Group B | Spirochaetes | Spirochaetales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_018526486.1 | Spirochaeta alkalica | [FeFe] Group B | Spirochaetes | Spirochaetales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_014270720.1 | Sphaerochaeta pleomorpha | [FeFe] Group B | Spirochaetes | Spirochaetales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_013608539.1 | Sphaerochaeta globosa | [FeFe] Group B | Spirochaetes | Spirochaetales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_006905160.1 | Shuttleworthia satelles | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_006190671.1 | Selenomonas sputigena | [FeFe] Group B | Firmicutes | Selenomonadales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_012862886.1 | Sebaldella termitidis | [FeFe] Group B | Fusobacteria | Fusobacteriales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_023920581.1 | Ruminococcus lactaris | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_028527755.1 | Ruminococcus gauvreauii | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_002848990.1 | Ruminococcus albus | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_027095964.1 | Ruminiclostridium viride | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_004627876.1 | Ruminiclostridium termitidis | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_015359535.1 | Ruminiclostridium stercorarium | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_015360284.1 | Ruminiclostridium stercorarium | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_020073353.1 | Ruminiclostridium sporosphaeroides | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_020073385.1 | Ruminiclostridium sporosphaeroides | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_015518171.1 | Ruminiclostridium siraeum | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_006352873.1 | Ruminiclostridium methylpentosum | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_003530568.1 | Ruminiclostridium leptum | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_003532170.1 | Ruminiclostridium leptum | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_027629995.1 | Ruminiclostridium cellobioparum | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_015560436.1 | Roseburia intestinalis | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_014079355.1 | Roseburia hominis | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_014080018.1 | Roseburia hominis | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_006599253.1 | Pseudoramibacter alactolyticus | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_006571748.1 | Pseudoflavonifractor capillosus | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_028830146.1 | Proteocatella sphenisci | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_025864379.1 | Prolixibacter bellariivorans | [FeFe] Group B | Bacteroidetes | Bacteroidales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
Literature
Genetics:
- Calusinska, M., Happe, T., Joris, B., and Wilmotte, A. (2010) The surprising diversity of clostridial hydrogenases: a comparative genomic perspective. Microbiol. 156: 1575-1588.
- Greening, C., Biswas, A., Carere, C.R., Jackson, C.J., Taylor, M.C., Stott, M.B., Cook, G.M., and Morales, S.E. (2016) Genomic and metagenomic surveys of hydrogenase distribution indicate H2 is a widely utilised energy source for microbial growth and survival. ISME J. 10: 761-777.
- Wolf, P.G., Biswas, A., Morales, S.E., Greening, C., and Gaskins, H.R. (2016) H2 metabolism is widespread and diverse among human colonic microbes. Gut Microb. 7: 235-245.
Physiology:
- Betian, H.G., Linehan, B.A., Bryant, M.P., and Holdeman, L.V. (1977) Isolation of a cellulolytic Bacteroides sp. from human feces. Appl. Environ. Microbiol. 33: 1009-1010.
- Fischbach, M.A. and Sonnenburg, J.L. (2011) Eating for two: how metabolism establishes interspecies interactions in the gut. Cell Host Microb. 10: 336-347.