(The tutorial is here. The documentation is here.)

Here is some advice in case you get into trouble using PriFi. If this page doesn't help you, please visit the Helpdesk.

PriFi doesn't find any primers in my sequences

What's a good way to relax the primer criteria?

Relaxing the criteria may lower the quality of any primers PriFi might find. However, if you think your alignment is good enough even though PriFi found no primers initially, you can relax the criteria after clicking the "Configure" button. Perhaps you simply disagree with some of the parameter settings defining "optimal" values; in that case you should of course alter them. Any changes to the default values have to be made every time you run PriFi and prior to uploading any file.

Other than that you might look around on this page where all parameters are explained.

I have trouble uploading a file

Go to the PriFi main page