[NiFe] Group 4h-hydrogenase
This entry was last updated at: Feb. 9, 2018, 6:24 a.m.
Properties
| Group | [NiFe] Group 4: Respiratory H2-evolving [NiFe] hydrogenases |
| Subgroup | [NiFe] Group 4h: Ferredoxin-coupled, Eha-type |
| Function | Forms supercomplex that couples H2 oxidation to ferredoxin reduction by utilising the sodium-motive force. The reduced ferredoxin generated can be used anaplerotically to replenish the Wolfe cycle of methanogenesis. While the reverse direction is energetically favourable, it is unclear whether it occurs physiologically. |
| Activity | H2-uptake |
| Oxygen tolerance | O2-sensitive |
| Localisation | Membrane-bound |
Distribution
| Ecosystem distribution | Hydrothermal systems, other high temperature ecosystems |
| Taxonomic distribution | Sporadically distributed in anaerobic bacteria and archaea |
Genetic Organisation
e.g. Methanothermobacter marburgensis (WP_013295617.1):
Architecture
| Structures |
|
| Subunits | 20 |
| Subunit description | EhaO (hydrogenase large subunit) EhaN (hydrogenase small subunit) EhaPQ (polyferredoxin subunits) EhaJ (NuoH-like transmembrane adaptor) EhaH (NuoL-like transmembrane adaptor) EhaABCDEFGIKLM (transmembrane subunits) EhaRST (conserved hypothetical subunits) |
| Catalytic site | [NiFe]-centre |
| FeS clusters | Proximal: 4Cys[4Fe4S] Medial: Absent Distal: Absent |
Sequences in this class
| NCBI Accession | Organism | Hydrogenase Class | Phylum | Order | Activity (Predicted) | Oxygen Tolerance (Predicted) | Subunits (Predicted) | Metal Centres (Predicted) | Accessory Subunits (Predicted) |
|---|---|---|---|---|---|---|---|---|---|
| WP_004031618.1 | Methanobacterium formicicum | [NiFe] Group 4h | Euryarchaeota | Methanobacteriales | Evolving | Sensitive | 20 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoH, NuoL, Two polyferredoxins, eleven TM proteins, three hypotheticals |
| WP_004038499.1 | Methanofollis liminatans | [NiFe] Group 4h | Euryarchaeota | Methanomicrobiales | Evolving | Sensitive | 20 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoH, NuoL, Two polyferredoxins, eleven TM proteins, three hypotheticals |
| WP_004076146.1 | Methanoplanus limicola | [NiFe] Group 4h | Euryarchaeota | Methanomicrobiales | Evolving | Sensitive | 20 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoH, NuoL, Two polyferredoxins, eleven TM proteins, three hypotheticals |
| WP_004592818.1 | Methanocaldococcus villosus | [NiFe] Group 4h | Euryarchaeota | Methanococcales | Evolving | Sensitive | 20 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoH, NuoL, Two polyferredoxins, eleven TM proteins, three hypotheticals |
| WP_007045054.1 | Methanotorris formicicus | [NiFe] Group 4h | Euryarchaeota | Methanococcales | Evolving | Sensitive | 20 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoH, NuoL, Two polyferredoxins, eleven TM proteins, three hypotheticals |
| WP_010870018.1 | Methanocaldococcus jannaschii | [NiFe] Group 4h | Euryarchaeota | Methanococcales | Evolving | Sensitive | 20 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoH, NuoL, Two polyferredoxins, eleven TM proteins, three hypotheticals |
| WP_010876037.1 | Methanothermobacter thermautotrophicus | [NiFe] Group 4h | Euryarchaeota | Methanobacteriales | Evolving | Sensitive | 20 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoH, NuoL, Two polyferredoxins, eleven TM proteins, three hypotheticals |
| WP_011018833.1 | Methanopyrus kandleri | [NiFe] Group 4h | Euryarchaeota | Methanopyrales | Evolving | Sensitive | 20 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoH, NuoL, Two polyferredoxins, eleven TM proteins, three hypotheticals |
| WP_011449071.1 | Methanospirillum hungatei | [NiFe] Group 4h | Euryarchaeota | Methanomicrobiales | Evolving | Sensitive | 20 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoH, NuoL, Two polyferredoxins, eleven TM proteins, three hypotheticals |
| WP_011832947.1 | Methanocorpusculum labreanum | [NiFe] Group 4h | Euryarchaeota | Methanomicrobiales | Evolving | Sensitive | 20 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoH, NuoL, Two polyferredoxins, eleven TM proteins, three hypotheticals |
| WP_011844026.1 | Methanoculleus marisnigri | [NiFe] Group 4h | Euryarchaeota | Methanomicrobiales | Evolving | Sensitive | 20 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoH, NuoL, Two polyferredoxins, eleven TM proteins, three hypotheticals |
| WP_011972581.1 | Methanococcus vannielii | [NiFe] Group 4h | Euryarchaeota | Methanococcales | Evolving | Sensitive | 20 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoH, NuoL, Two polyferredoxins, eleven TM proteins, three hypotheticals |
| WP_011973090.1 | Methanococcus aeolicus | [NiFe] Group 4h | Euryarchaeota | Methanococcales | Evolving | Sensitive | 20 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoH, NuoL, Two polyferredoxins, eleven TM proteins, three hypotheticals |
| WP_012193964.1 | Methanococcus maripaludis | [NiFe] Group 4h | Euryarchaeota | Methanococcales | Evolving | Sensitive | 20 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoH, NuoL, Two polyferredoxins, eleven TM proteins, three hypotheticals |
| WP_012956197.1 | Methanobrevibacter ruminantium | [NiFe] Group 4h | Euryarchaeota | Methanobacteriales | Evolving | Sensitive | 20 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoH, NuoL, Two polyferredoxins, eleven TM proteins, three hypotheticals |
| WP_013100690.1 | Methanocaldococcus infernus | [NiFe] Group 4h | Euryarchaeota | Methanococcales | Evolving | Sensitive | 20 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoH, NuoL, Two polyferredoxins, eleven TM proteins, three hypotheticals |
| WP_013180984.1 | Methanococcus voltae | [NiFe] Group 4h | Euryarchaeota | Methanococcales | Evolving | Sensitive | 20 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoH, NuoL, Two polyferredoxins, eleven TM proteins, three hypotheticals |
| WP_013295617.1 | Methanothermobacter marburgensis | [NiFe] Group 4h | Euryarchaeota | Methanobacteriales | Evolving | Sensitive | 20 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoH, NuoL, Two polyferredoxins, eleven TM proteins, three hypotheticals |
| WP_013329140.1 | Methanoplanus petrolearius | [NiFe] Group 4h | Euryarchaeota | Methanomicrobiales | Evolving | Sensitive | 20 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoH, NuoL, Two polyferredoxins, eleven TM proteins, three hypotheticals |
| WP_013644869.1 | Methanobacterium lacus | [NiFe] Group 4h | Euryarchaeota | Methanobacteriales | Evolving | Sensitive | 20 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoH, NuoL, Two polyferredoxins, eleven TM proteins, three hypotheticals |
| WP_013799750.1 | Methanotorris igneus | [NiFe] Group 4h | Euryarchaeota | Methanococcales | Evolving | Sensitive | 20 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoH, NuoL, Two polyferredoxins, eleven TM proteins, three hypotheticals |
| WP_013825480.1 | Methanobacterium paludis | [NiFe] Group 4h | Euryarchaeota | Methanobacteriales | Evolving | Sensitive | 20 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoH, NuoL, Two polyferredoxins, eleven TM proteins, three hypotheticals |
| WP_013867209.1 | Methanothermococcus okinawensis | [NiFe] Group 4h | Euryarchaeota | Methanococcales | Evolving | Sensitive | 20 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoH, NuoL, Two polyferredoxins, eleven TM proteins, three hypotheticals |
| WP_014867151.1 | Methanoculleus bourgensis | [NiFe] Group 4h | Euryarchaeota | Methanomicrobiales | Evolving | Sensitive | 20 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoH, NuoL, Two polyferredoxins, eleven TM proteins, three hypotheticals |
| WP_015732568.1 | Methanocaldococcus vulcanius | [NiFe] Group 4h | Euryarchaeota | Methanococcales | Evolving | Sensitive | 20 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoH, NuoL, Two polyferredoxins, eleven TM proteins, three hypotheticals |
| WP_015790909.1 | Methanocaldococcus fervens | [NiFe] Group 4h | Euryarchaeota | Methanococcales | Evolving | Sensitive | 20 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoH, NuoL, Two polyferredoxins, eleven TM proteins, three hypotheticals |
| WP_016358530.1 | Methanobrevibacter sp. AbM4 | [NiFe] Group 4h | Euryarchaeota | Methanobacteriales | Evolving | Sensitive | 20 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoH, NuoL, Two polyferredoxins, eleven TM proteins, three hypotheticals |
| WP_018154556.1 | Methanothermococcus thermolithotrophicus | [NiFe] Group 4h | Euryarchaeota | Methanococcales | Evolving | Sensitive | 20 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoH, NuoL, Two polyferredoxins, eleven TM proteins, three hypotheticals |
| WP_019266264.1 | Methanobrevibacter smithii | [NiFe] Group 4h | Euryarchaeota | Methanobacteriales | Evolving | Sensitive | 20 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoH, NuoL, Two polyferredoxins, eleven TM proteins, three hypotheticals |
| WP_023992259.1 | Methanobacterium sp. MB1 | [NiFe] Group 4h | Euryarchaeota | Methanobacteriales | Evolving | Sensitive | 20 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoH, NuoL, Two polyferredoxins, eleven TM proteins, three hypotheticals |
Literature
Genetics:
- Søndergaard, D., Petersen, C.N.S., and Greening, C. (2017) HydDB: a web tool for hydrogenase classification and analysis. Sci. Rep. 6: 34212.
- Tersteegen, A. and Hedderich, R. (1999) Methanobacterium thermoautotrophicum encodes two multisubunit membrane-bound [NiFe] hydrogenases. Eur. J. Biochem. 264: 930-943.
Physiology:
- Lie, T.J., Costa, K.C., Lupa, B., Korpole, S., Whitman, W.B. and Leigh, J.A. (2012) Essential anaplerotic role for the energy-converting hydrogenase Eha in hydrogenotrophic methanogenesis. Proc. Natl. Acad. Sci. USA 109: 15473-15478.
- Thauer, R.K., 2012. The Wolfe cycle comes full circle. Proc. Acad. Natl. Sci. USA 109: 15084-15085.
- Thauer, R.K., Kaster, A.K., Seedorf, H., Buckel, W., and Hedderich, R. (2008) Methanogenic archaea: ecologically relevant differences in energy conservation. Nat. Rev. Microbiol. 6: 579-591.