[NiFe] Group 4e-hydrogenase

This entry was last updated at: June 13, 2016, 11:11 a.m.

Properties
Group [NiFe] Group 4: Respiratory H2-evolving [NiFe] hydrogenases
Subgroup [NiFe] Group 4e: Ferredoxin-coupled, Ech-type
Function Forms respiratory complex that couples oxidation of ferredoxinred to reduction of protons to H2 in acetoclastic methanogenesis. This process appears to be coupled to sodium or proton translocation through the transmembrane modules. The complex can also act in the reverse direction during hydrogenotrophic methanogenesis.
Activity Bidirectional
Oxygen tolerance O2-sensitive
Localisation Membrane-bound
Distribution
Ecosystem distribution Anoxic soils and sediments, animal gastrointestinal tracts
Taxonomic distribution Encoded in multiple methanogenic orders and anaerobic bacteria
Genetic Organisation

e.g. Methanosarcina barkeri (WP_011305188.1):

Architecture
Structures
    None
Subunits 6
Subunit description EchA (hydrogenase large subunit)
EchC (hydrogenase small subunit)
EchF (iron-sulfur subunit)
EchB (NuoH-like transmembrane adaptor)
EchA (NuoL-like transmembrane adaptor)
EchD (NuoC-like transmembrane adaptor)
Catalytic site [NiFe]-centre
FeS clusters Proximal: 4Cys[4Fe4S]
Medial: Absent
Distal: Absent
Sequences in this class
NCBI AccessionOrganismHydrogenase ClassPhylumOrderActivity (Predicted)Oxygen Tolerance (Predicted)Subunits (Predicted)Metal Centres (Predicted)Accessory Subunits (Predicted)
WP_011448732.1Methanospirillum hungatei[NiFe] Group 4eEuryarchaeotaMethanomicrobialesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_012663581.1Nautilia profundicola[NiFe] Group 4eEpsilonproteobacteriaNautilialesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_012376920.1Opitutus terrae[NiFe] Group 4eVerrucomicrobiaOpitutalesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_028241974.1Pseudobutyrivibrio ruminis[NiFe] Group 4eFirmicutesClostridialesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_028246016.1Pseudobutyrivibrio ruminis[NiFe] Group 4eFirmicutesClostridialesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_028234750.1Pseudobutyrivibrio sp. MD2005[NiFe] Group 4eFirmicutesClostridialesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_003514863.1Ruminiclostridium thermocellum[NiFe] Group 4eFirmicutesClostridialesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_006361427.1Slackia exigua[NiFe] Group 4eActinobacteriaCoriobacterialesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_012798463.1Slackia heliotrinireducens[NiFe] Group 4eActinobacteriaCoriobacterialesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_009138996.1Slackia piriformis[NiFe] Group 4eActinobacteriaCoriobacterialesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_009078628.1Slackia sp. CM382[NiFe] Group 4eActinobacteriaCoriobacterialesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_022790212.1Streptococcus pleomorphus[NiFe] Group 4eFirmicutesLactobacillialesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_007047619.1Subdoligranulum variabile[NiFe] Group 4eFirmicutesClostridialesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_013460916.1Sulfuricurvum kujiense[NiFe] Group 4eEpsilonproteobacteriaCampylobacteralesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_015654365.1Sulfuricurvum sp. RIFRC-1[NiFe] Group 4eEpsilonproteobacteriaCampylobacteralesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_008340210.1Sulfurimonas gotlandica[NiFe] Group 4eEpsilonproteobacteriaCampylobacteralesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_013625216.1Syntrophobotulus glycolicus[NiFe] Group 4eFirmicutesClostridialesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_013174844.1Syntrophothermus lipocalidus[NiFe] Group 4eFirmicutesClostridialesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_015051201.1Thermacetogenium phaeum[NiFe] Group 4eFirmicutesThermoanaerobacteralesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_003868941.1Thermoanaerobacter ethanolicus[NiFe] Group 4eFirmicutesThermoanaerobacteralesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_019907186.1Thermoanaerobacter indiensis[NiFe] Group 4eFirmicutesThermoanaerobacteralesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_012995592.1Thermoanaerobacter italicus[NiFe] Group 4eFirmicutesThermoanaerobacteralesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_006569905.1Thermoanaerobacter siderophilus[NiFe] Group 4eFirmicutesThermoanaerobacteralesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_029688128.1Thermoanaerobacter sp. A7A[NiFe] Group 4eFirmicutesThermoanaerobacteralesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_004399987.1Thermoanaerobacter thermohydrosulfuricus[NiFe] Group 4eFirmicutesThermoanaerobacteralesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_014062054.1Thermoanaerobacter wiegelii[NiFe] Group 4eFirmicutesThermoanaerobacteralesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_014757614.1Thermoanaerobacterium saccharolyticum[NiFe] Group 4eFirmicutesThermoanaerobacteralesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_013296689.1Thermoanaerobacterium thermosaccharolyticum[NiFe] Group 4eFirmicutesThermoanaerobacteralesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_013786935.1Thermoanaerobacterium xylanolyticum[NiFe] Group 4eFirmicutesThermoanaerobacteralesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_013537236.1Thermovibrio ammonificans[NiFe] Group 4eAquificaeDesulfobacteralesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_014777057.1Thiocystis violascens[NiFe] Group 4eGammaproteobacteriaChromatialesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_015282154.1Thioflavicoccus mobilis[NiFe] Group 4eGammaproteobacteriaChromatialesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein

Literature

Genetics:

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Biochemistry: