[NiFe] Group 4e-hydrogenase

This entry was last updated at: June 13, 2016, 11:11 a.m.

Properties
Group [NiFe] Group 4: Respiratory H2-evolving [NiFe] hydrogenases
Subgroup [NiFe] Group 4e: Ferredoxin-coupled, Ech-type
Function Forms respiratory complex that couples oxidation of ferredoxinred to reduction of protons to H2 in acetoclastic methanogenesis. This process appears to be coupled to sodium or proton translocation through the transmembrane modules. The complex can also act in the reverse direction during hydrogenotrophic methanogenesis.
Activity Bidirectional
Oxygen tolerance O2-sensitive
Localisation Membrane-bound
Distribution
Ecosystem distribution Anoxic soils and sediments, animal gastrointestinal tracts
Taxonomic distribution Encoded in multiple methanogenic orders and anaerobic bacteria
Genetic Organisation

e.g. Methanosarcina barkeri (WP_011305188.1):

Architecture
Structures
    None
Subunits 6
Subunit description EchA (hydrogenase large subunit)
EchC (hydrogenase small subunit)
EchF (iron-sulfur subunit)
EchB (NuoH-like transmembrane adaptor)
EchA (NuoL-like transmembrane adaptor)
EchD (NuoC-like transmembrane adaptor)
Catalytic site [NiFe]-centre
FeS clusters Proximal: 4Cys[4Fe4S]
Medial: Absent
Distal: Absent
Sequences in this class
NCBI AccessionOrganismHydrogenase ClassPhylumOrderActivity (Predicted)Oxygen Tolerance (Predicted)Subunits (Predicted)Metal Centres (Predicted)Accessory Subunits (Predicted)
WP_012619310.1Methanosphaerula palustris[NiFe] Group 4eEuryarchaeotaMethanomicrobialesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_011448732.1Methanospirillum hungatei[NiFe] Group 4eEuryarchaeotaMethanomicrobialesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_011305188.1Methanosarcina barkeri[NiFe] Group 4eEuryarchaeotaMethanosarcinalesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_011034248.1Methanosarcina mazei[NiFe] Group 4eEuryarchaeotaMethanosarcinalesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_007474009.1Caminibacter mediatlanticus[NiFe] Group 4eEpsilonproteobacteriaNautilialesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_024789181.1Lebetimonas sp. JH292[NiFe] Group 4eEpsilonproteobacteriaNautilialesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_012663581.1Nautilia profundicola[NiFe] Group 4eEpsilonproteobacteriaNautilialesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_012376920.1Opitutus terrae[NiFe] Group 4eVerrucomicrobiaOpitutalesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_007070913.1Dialister invisus[NiFe] Group 4eFirmicutesSelenomonadalesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_022106764.1Dialister sp. CAG:486[NiFe] Group 4eFirmicutesSelenomonadalesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_008860581.1Dialister succinatiphilus[NiFe] Group 4eFirmicutesSelenomonadalesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_011024634.1Caldanaerobacter subterraneus[NiFe] Group 4eFirmicutesThermoanaerobacteralesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_026486855.1Caldanaerobius polysaccharolyticus[NiFe] Group 4eFirmicutesThermoanaerobacteralesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_029228227.1Caldicellulosiruptor acetigenus[NiFe] Group 4eFirmicutesThermoanaerobacteralesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_013403527.1Caldicellulosiruptor hydrothermalis[NiFe] Group 4eFirmicutesThermoanaerobacteralesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_013432390.1Caldicellulosiruptor kristjanssonii[NiFe] Group 4eFirmicutesThermoanaerobacteralesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_014042067.1Caldicellulosiruptor lactoaceticus[NiFe] Group 4eFirmicutesThermoanaerobacteralesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_013290727.1Caldicellulosiruptor obsidiansis[NiFe] Group 4eFirmicutesThermoanaerobacteralesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_013411862.1Caldicellulosiruptor owensensis[NiFe] Group 4eFirmicutesThermoanaerobacteralesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_011917066.1Caldicellulosiruptor saccharolyticus[NiFe] Group 4eFirmicutesThermoanaerobacteralesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_018962513.1Coprothermobacter platensis[NiFe] Group 4eFirmicutesThermoanaerobacteralesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_015051201.1Thermacetogenium phaeum[NiFe] Group 4eFirmicutesThermoanaerobacteralesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_003868941.1Thermoanaerobacter ethanolicus[NiFe] Group 4eFirmicutesThermoanaerobacteralesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_019907186.1Thermoanaerobacter indiensis[NiFe] Group 4eFirmicutesThermoanaerobacteralesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_012995592.1Thermoanaerobacter italicus[NiFe] Group 4eFirmicutesThermoanaerobacteralesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_006569905.1Thermoanaerobacter siderophilus[NiFe] Group 4eFirmicutesThermoanaerobacteralesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_029688128.1Thermoanaerobacter sp. A7A[NiFe] Group 4eFirmicutesThermoanaerobacteralesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_004399987.1Thermoanaerobacter thermohydrosulfuricus[NiFe] Group 4eFirmicutesThermoanaerobacteralesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_014062054.1Thermoanaerobacter wiegelii[NiFe] Group 4eFirmicutesThermoanaerobacteralesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_014757614.1Thermoanaerobacterium saccharolyticum[NiFe] Group 4eFirmicutesThermoanaerobacteralesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_013296689.1Thermoanaerobacterium thermosaccharolyticum[NiFe] Group 4eFirmicutesThermoanaerobacteralesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_013786935.1Thermoanaerobacterium xylanolyticum[NiFe] Group 4eFirmicutesThermoanaerobacteralesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein

Literature

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