This entry was last updated at: June 13, 2016, 11:11 a.m.
Group | [NiFe] Group 4: Respiratory H2-evolving [NiFe] hydrogenases |
Subgroup | [NiFe] Group 4e: Ferredoxin-coupled, Ech-type |
Function | Forms respiratory complex that couples oxidation of ferredoxinred to reduction of protons to H2 in acetoclastic methanogenesis. This process appears to be coupled to sodium or proton translocation through the transmembrane modules. The complex can also act in the reverse direction during hydrogenotrophic methanogenesis. |
Activity | Bidirectional |
Oxygen tolerance | O2-sensitive |
Localisation | Membrane-bound |
Ecosystem distribution | Anoxic soils and sediments, animal gastrointestinal tracts |
Taxonomic distribution | Encoded in multiple methanogenic orders and anaerobic bacteria |
e.g. Methanosarcina barkeri (WP_011305188.1):
Structures |
|
Subunits | 6 |
Subunit description | EchA (hydrogenase large subunit) EchC (hydrogenase small subunit) EchF (iron-sulfur subunit) EchB (NuoH-like transmembrane adaptor) EchA (NuoL-like transmembrane adaptor) EchD (NuoC-like transmembrane adaptor) |
Catalytic site | [NiFe]-centre |
FeS clusters | Proximal: 4Cys[4Fe4S] Medial: Absent Distal: Absent |
NCBI Accession | Organism | Hydrogenase Class | Phylum | Order | Activity (Predicted) | Oxygen Tolerance (Predicted) | Subunits (Predicted) | Metal Centres (Predicted) | Accessory Subunits (Predicted) |
---|---|---|---|---|---|---|---|---|---|
WP_012619310.1 | Methanosphaerula palustris | [NiFe] Group 4e | Euryarchaeota | Methanomicrobiales | Evolving | Sensitive | 6 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, NuoC, [FeS] protein |
WP_011448732.1 | Methanospirillum hungatei | [NiFe] Group 4e | Euryarchaeota | Methanomicrobiales | Evolving | Sensitive | 6 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, NuoC, [FeS] protein |
WP_011305188.1 | Methanosarcina barkeri | [NiFe] Group 4e | Euryarchaeota | Methanosarcinales | Evolving | Sensitive | 6 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, NuoC, [FeS] protein |
WP_011034248.1 | Methanosarcina mazei | [NiFe] Group 4e | Euryarchaeota | Methanosarcinales | Evolving | Sensitive | 6 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, NuoC, [FeS] protein |
WP_007474009.1 | Caminibacter mediatlanticus | [NiFe] Group 4e | Epsilonproteobacteria | Nautiliales | Evolving | Sensitive | 6 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, NuoC, [FeS] protein |
WP_024789181.1 | Lebetimonas sp. JH292 | [NiFe] Group 4e | Epsilonproteobacteria | Nautiliales | Evolving | Sensitive | 6 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, NuoC, [FeS] protein |
WP_012663581.1 | Nautilia profundicola | [NiFe] Group 4e | Epsilonproteobacteria | Nautiliales | Evolving | Sensitive | 6 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, NuoC, [FeS] protein |
WP_012376920.1 | Opitutus terrae | [NiFe] Group 4e | Verrucomicrobia | Opitutales | Evolving | Sensitive | 6 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, NuoC, [FeS] protein |
WP_007070913.1 | Dialister invisus | [NiFe] Group 4e | Firmicutes | Selenomonadales | Evolving | Sensitive | 6 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, NuoC, [FeS] protein |
WP_022106764.1 | Dialister sp. CAG:486 | [NiFe] Group 4e | Firmicutes | Selenomonadales | Evolving | Sensitive | 6 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, NuoC, [FeS] protein |
WP_008860581.1 | Dialister succinatiphilus | [NiFe] Group 4e | Firmicutes | Selenomonadales | Evolving | Sensitive | 6 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, NuoC, [FeS] protein |
WP_011024634.1 | Caldanaerobacter subterraneus | [NiFe] Group 4e | Firmicutes | Thermoanaerobacterales | Evolving | Sensitive | 6 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, NuoC, [FeS] protein |
WP_026486855.1 | Caldanaerobius polysaccharolyticus | [NiFe] Group 4e | Firmicutes | Thermoanaerobacterales | Evolving | Sensitive | 6 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, NuoC, [FeS] protein |
WP_029228227.1 | Caldicellulosiruptor acetigenus | [NiFe] Group 4e | Firmicutes | Thermoanaerobacterales | Evolving | Sensitive | 6 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, NuoC, [FeS] protein |
WP_013403527.1 | Caldicellulosiruptor hydrothermalis | [NiFe] Group 4e | Firmicutes | Thermoanaerobacterales | Evolving | Sensitive | 6 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, NuoC, [FeS] protein |
WP_013432390.1 | Caldicellulosiruptor kristjanssonii | [NiFe] Group 4e | Firmicutes | Thermoanaerobacterales | Evolving | Sensitive | 6 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, NuoC, [FeS] protein |
WP_014042067.1 | Caldicellulosiruptor lactoaceticus | [NiFe] Group 4e | Firmicutes | Thermoanaerobacterales | Evolving | Sensitive | 6 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, NuoC, [FeS] protein |
WP_013290727.1 | Caldicellulosiruptor obsidiansis | [NiFe] Group 4e | Firmicutes | Thermoanaerobacterales | Evolving | Sensitive | 6 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, NuoC, [FeS] protein |
WP_013411862.1 | Caldicellulosiruptor owensensis | [NiFe] Group 4e | Firmicutes | Thermoanaerobacterales | Evolving | Sensitive | 6 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, NuoC, [FeS] protein |
WP_011917066.1 | Caldicellulosiruptor saccharolyticus | [NiFe] Group 4e | Firmicutes | Thermoanaerobacterales | Evolving | Sensitive | 6 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, NuoC, [FeS] protein |
WP_018962513.1 | Coprothermobacter platensis | [NiFe] Group 4e | Firmicutes | Thermoanaerobacterales | Evolving | Sensitive | 6 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, NuoC, [FeS] protein |
WP_015051201.1 | Thermacetogenium phaeum | [NiFe] Group 4e | Firmicutes | Thermoanaerobacterales | Evolving | Sensitive | 6 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, NuoC, [FeS] protein |
WP_003868941.1 | Thermoanaerobacter ethanolicus | [NiFe] Group 4e | Firmicutes | Thermoanaerobacterales | Evolving | Sensitive | 6 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, NuoC, [FeS] protein |
WP_019907186.1 | Thermoanaerobacter indiensis | [NiFe] Group 4e | Firmicutes | Thermoanaerobacterales | Evolving | Sensitive | 6 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, NuoC, [FeS] protein |
WP_012995592.1 | Thermoanaerobacter italicus | [NiFe] Group 4e | Firmicutes | Thermoanaerobacterales | Evolving | Sensitive | 6 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, NuoC, [FeS] protein |
WP_006569905.1 | Thermoanaerobacter siderophilus | [NiFe] Group 4e | Firmicutes | Thermoanaerobacterales | Evolving | Sensitive | 6 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, NuoC, [FeS] protein |
WP_029688128.1 | Thermoanaerobacter sp. A7A | [NiFe] Group 4e | Firmicutes | Thermoanaerobacterales | Evolving | Sensitive | 6 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, NuoC, [FeS] protein |
WP_004399987.1 | Thermoanaerobacter thermohydrosulfuricus | [NiFe] Group 4e | Firmicutes | Thermoanaerobacterales | Evolving | Sensitive | 6 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, NuoC, [FeS] protein |
WP_014062054.1 | Thermoanaerobacter wiegelii | [NiFe] Group 4e | Firmicutes | Thermoanaerobacterales | Evolving | Sensitive | 6 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, NuoC, [FeS] protein |
WP_014757614.1 | Thermoanaerobacterium saccharolyticum | [NiFe] Group 4e | Firmicutes | Thermoanaerobacterales | Evolving | Sensitive | 6 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, NuoC, [FeS] protein |
WP_013296689.1 | Thermoanaerobacterium thermosaccharolyticum | [NiFe] Group 4e | Firmicutes | Thermoanaerobacterales | Evolving | Sensitive | 6 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, NuoC, [FeS] protein |
WP_013786935.1 | Thermoanaerobacterium xylanolyticum | [NiFe] Group 4e | Firmicutes | Thermoanaerobacterales | Evolving | Sensitive | 6 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, NuoC, [FeS] protein |
Genetics:
Physiology:
Biochemistry: