[NiFe] Group 4d-hydrogenase

This entry was last updated at: May 22, 2018, 1:09 a.m.

Properties
Group [NiFe] Group 4: Respiratory H2-evolving [NiFe] hydrogenase
Subgroup [NiFe] Group 4d: Ferredoxin-coupled, Mrp-linked
Function Forms respiratory supercomplex that couples oxidation of ferredoxinred to reduction of protons to H2. The energy released is used to translocate Na+ ions through Mrp-like antiporter subunits to generate a sodium-motive force.
Activity H2-evolution
Oxygen tolerance O2-sensitive
Localisation Membrane-bound
Distribution
Ecosystem distribution Hydrothermal vents, other high temperature ecosystems
Taxonomic distribution Exclusive to Thermococci and Aciduliprofundi
Genetic Organisation

e.g. Pyrococcus furiosus (WP_011012581.1):

Architecture
Structures
  • 6CFW (Pyrococcus furiosus, 3.7 Å resolution)
Subunits 14
Subunit description MbhL (hydrogenase large subunit)
MbhJ (hydrogenase small subunit)
MbhM (NuoH-like transmembrane adaptor)
MbhH (NuoL-like transmembrane adaptor)
MchK (NuoC-like transmembrane adaptor)
MchABCDEFG (Mrp-like antiporter subunits)
Catalytic site [NiFe]-centre
FeS clusters Proximal: 4Cys[4Fe4S]
Medial: Absent
Distal: Absent
Sequences in this class
NCBI AccessionOrganismHydrogenase ClassPhylumOrderActivity (Predicted)Oxygen Tolerance (Predicted)Subunits (Predicted)Metal Centres (Predicted)Accessory Subunits (Predicted)
WP_011833332.1Methanocorpusculum labreanum[NiFe] Group 4dEuryarchaeotaMethanomicrobialesEvolvingSensitive13[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, two [FeS] proteins, seven MRP antiporter subunits, etc.
WP_004038443.1Methanofollis liminatans[NiFe] Group 4dEuryarchaeotaMethanomicrobialesEvolvingSensitive13[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, two [FeS] proteins, seven MRP antiporter subunits, etc.
WP_004077410.1Methanoplanus limicola[NiFe] Group 4dEuryarchaeotaMethanomicrobialesEvolvingSensitive13[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, two [FeS] proteins, seven MRP antiporter subunits, etc.
WP_013329507.1Methanoplanus petrolearius[NiFe] Group 4dEuryarchaeotaMethanomicrobialesEvolvingSensitive13[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, two [FeS] proteins, seven MRP antiporter subunits, etc.
WP_011449544.1Methanospirillum hungatei[NiFe] Group 4dEuryarchaeotaMethanomicrobialesEvolvingSensitive13[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, two [FeS] proteins, seven MRP antiporter subunits, etc.
WP_029166411.1Aminiphilus circumscriptus[NiFe] Group 4dSynergistetesSynergistalesEvolvingSensitive13[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, two [FeS] proteins, seven MRP antiporter subunits, etc.
WP_013048648.1Aminobacterium colombiense[NiFe] Group 4dSynergistetesSynergistalesEvolvingSensitive13[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, two [FeS] proteins, seven MRP antiporter subunits, etc.
WP_024821984.1Aminobacterium mobile[NiFe] Group 4dSynergistetesSynergistalesEvolvingSensitive13[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, two [FeS] proteins, seven MRP antiporter subunits, etc.
WP_006301161.1Aminomonas paucivorans[NiFe] Group 4dSynergistetesSynergistalesEvolvingSensitive13[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, two [FeS] proteins, seven MRP antiporter subunits, etc.
WP_009200527.1Anaerobaculum hydrogeniformans[NiFe] Group 4dSynergistetesSynergistalesEvolvingSensitive13[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, two [FeS] proteins, seven MRP antiporter subunits, etc.
WP_014807277.1Anaerobaculum mobile[NiFe] Group 4dSynergistetesSynergistalesEvolvingSensitive13[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, two [FeS] proteins, seven MRP antiporter subunits, etc.
WP_005662523.1Dethiosulfovibrio peptidovorans[NiFe] Group 4dSynergistetesSynergistalesEvolvingSensitive13[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, two [FeS] proteins, seven MRP antiporter subunits, etc.
WP_009164181.1Pyramidobacter piscolens[NiFe] Group 4dSynergistetesSynergistalesEvolvingSensitive13[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, two [FeS] proteins, seven MRP antiporter subunits, etc.
WP_014163350.1Thermovirga lienii[NiFe] Group 4dSynergistetesSynergistalesEvolvingSensitive13[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, two [FeS] proteins, seven MRP antiporter subunits, etc.
WP_014122750.1Thermococcus sp. AM4[NiFe] Group 4dEuryarchaeotaThermococcalesEvolvingSensitive13[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, two [FeS] proteins, seven MRP antiporter subunits, etc.
WP_010867842.1Pyrococcus abyssi[NiFe] Group 4dEuryarchaeotaThermococcalesEvolvingSensitive13[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, two [FeS] proteins, seven MRP antiporter subunits, etc.
WP_011012581.1Pyrococcus furiosus[NiFe] Group 4dEuryarchaeotaThermococcalesEvolvingSensitive13[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, two [FeS] proteins, seven MRP antiporter subunits, etc.
WP_010885519.1Pyrococcus horikoshii[NiFe] Group 4dEuryarchaeotaThermococcalesEvolvingSensitive13[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, two [FeS] proteins, seven MRP antiporter subunits, etc.
WP_013747503.1Pyrococcus sp. NA2[NiFe] Group 4dEuryarchaeotaThermococcalesEvolvingSensitive13[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, two [FeS] proteins, seven MRP antiporter subunits, etc.
WP_014734412.1Pyrococcus sp. ST04[NiFe] Group 4dEuryarchaeotaThermococcalesEvolvingSensitive13[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, two [FeS] proteins, seven MRP antiporter subunits, etc.
WP_013905871.1Pyrococcus yayanosii[NiFe] Group 4dEuryarchaeotaThermococcalesEvolvingSensitive13[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, two [FeS] proteins, seven MRP antiporter subunits, etc.
WP_013467760.1Thermococcus barophilus[NiFe] Group 4dEuryarchaeotaThermococcalesEvolvingSensitive13[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, two [FeS] proteins, seven MRP antiporter subunits, etc.
WP_012751197.1Thermococcus gammatolerans[NiFe] Group 4dEuryarchaeotaThermococcalesEvolvingSensitive13[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, two [FeS] proteins, seven MRP antiporter subunits, etc.
WP_011251041.1Thermococcus kodakarensis[NiFe] Group 4dEuryarchaeotaThermococcalesEvolvingSensitive13[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, two [FeS] proteins, seven MRP antiporter subunits, etc.
WP_004066437.1Thermococcus litoralis[NiFe] Group 4dEuryarchaeotaThermococcalesEvolvingSensitive13[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, two [FeS] proteins, seven MRP antiporter subunits, etc.
WP_004066458.1Thermococcus litoralis[NiFe] Group 4dEuryarchaeotaThermococcalesEvolvingSensitive13[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, two [FeS] proteins, seven MRP antiporter subunits, etc.
WP_012572555.1Thermococcus onnurineus[NiFe] Group 4dEuryarchaeotaThermococcalesEvolvingSensitive13[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, two [FeS] proteins, seven MRP antiporter subunits, etc.
WP_015849571.1Thermococcus sibiricus[NiFe] Group 4dEuryarchaeotaThermococcalesEvolvingSensitive13[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, two [FeS] proteins, seven MRP antiporter subunits, etc.
WP_014013072.1Thermococcus sp. 4557[NiFe] Group 4dEuryarchaeotaThermococcalesEvolvingSensitive13[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, two [FeS] proteins, seven MRP antiporter subunits, etc.
WP_014013086.1Thermococcus sp. 4557[NiFe] Group 4dEuryarchaeotaThermococcalesEvolvingSensitive13[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, two [FeS] proteins, seven MRP antiporter subunits, etc.
WP_010479563.1Thermococcus zilligii[NiFe] Group 4dEuryarchaeotaThermococcalesEvolvingSensitive13[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, two [FeS] proteins, seven MRP antiporter subunits, etc.
WP_008083483.1Aciduliprofundum boonei[NiFe] Group 4dEuryarchaeotaUnclassifiedEvolvingSensitive13[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, two [FeS] proteins, seven MRP antiporter subunits, etc.
WP_015283654.1Aciduliprofundum sp. MAR08-339[NiFe] Group 4dEuryarchaeotaUnclassifiedEvolvingSensitive13[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, two [FeS] proteins, seven MRP antiporter subunits, etc.

Literature

Genetics:

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Biochemistry: