This entry was last updated at: Feb. 9, 2018, 6:24 a.m.
Group | [NiFe] Group 4: Respiratory H2-evolving [NiFe] hydrogenases |
Subgroup | [NiFe] Group 4b: Formate-respiring |
Function | Forms respiratory supercomplex that couples oxidation of low-potential carbon compounds to reduction of protons concomitant with Na+ translocation. Electron-input modules include formate dehydrogenases, carbon monoxide dehydrogenases, and potentially glutamate synthases. Electrons flow through hydrogenase resulting in proton reduction to H2. The energy released is used to translocate Na+ ions through Mrp-like antiporter subunits to generate a sodium-motive force. |
Activity | H2-evolution |
Oxygen tolerance | O2-sensitive |
Localisation | Membrane-bound |
Ecosystem distribution | Hydrothermal systems, other high temperature environments |
Taxonomic distribution | Encoded in Thermococci, Thermoprotei, and Thermodesulfovibrio |
e.g. Pyrococcus abyssi (WP_010868591.1):
Structures |
|
Subunits | 13+ |
Subunit description | MchD (hydrogenase large subunit) MchF (hydrogenase small subunit) MchC (NuoH-like transmembrane adaptor) MchAB (NuoL-like transmembrane adaptors) MchE (NuoC-like transmembrane adaptor) MchHIJKLMN (Mrp-like antiporter subunits) Form functional complexes with formate dehydrogenases, carbon monoxide dehydrogenases, and glutamate synthases |
Catalytic site | [NiFe]-centre |
FeS clusters | Proximal: 4Cys[4Fe4S] Medial: Absent Distal: Absent |
NCBI Accession | Organism | Hydrogenase Class | Phylum | Order | Activity (Predicted) | Oxygen Tolerance (Predicted) | Subunits (Predicted) | Metal Centres (Predicted) | Accessory Subunits (Predicted) |
---|---|---|---|---|---|---|---|---|---|
WP_028450152.1 | Chitinibacter tainanensis | [NiFe] Group 4b | Betaproteobacteria | Neisseriales | Evolving | Sensitive | 13 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, NuoC, two [FeS] proteins, seven MRP antiporter subunits, etc. |
WP_028451288.1 | Chitinilyticum aquatile | [NiFe] Group 4b | Betaproteobacteria | Neisseriales | Evolving | Sensitive | 13 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, NuoC, two [FeS] proteins, seven MRP antiporter subunits, etc. |
WP_026263170.1 | Chitiniphilus shinanonensis | [NiFe] Group 4b | Betaproteobacteria | Neisseriales | Evolving | Sensitive | 13 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, NuoC, two [FeS] proteins, seven MRP antiporter subunits, etc. |
WP_014767907.1 | Desulfurococcus fermentans | [NiFe] Group 4b | Crenarchaeota | Desulfurococcales | Evolving | Sensitive | 13 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, NuoC, two [FeS] proteins, seven MRP antiporter subunits, etc. |
WP_012608688.1 | Desulfurococcus kamchatkensis | [NiFe] Group 4b | Crenarchaeota | Desulfurococcales | Evolving | Sensitive | 13 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, NuoC, two [FeS] proteins, seven MRP antiporter subunits, etc. |
WP_013561596.1 | Desulfurococcus mucosus | [NiFe] Group 4b | Crenarchaeota | Desulfurococcales | Evolving | Sensitive | 13 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, NuoC, two [FeS] proteins, seven MRP antiporter subunits, etc. |
WP_020505780.1 | Lamprocystis purpurea | [NiFe] Group 4b | Gammaproteobacteria | Chromatiales | Evolving | Sensitive | 13 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, NuoC, two [FeS] proteins, seven MRP antiporter subunits, etc. |
WP_010868591.1 | Pyrococcus abyssi | [NiFe] Group 4b | Euryarchaeota | Thermococcales | Evolving | Sensitive | 13 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, NuoC, two [FeS] proteins, seven MRP antiporter subunits, etc. |
WP_013905846.1 | Pyrococcus yayanosii | [NiFe] Group 4b | Euryarchaeota | Thermococcales | Evolving | Sensitive | 13 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, NuoC, two [FeS] proteins, seven MRP antiporter subunits, etc. |
WP_011388069.1 | Rhodospirillum rubrum | [NiFe] Group 4b | Alphaproteobacteria | Rhodospirillales | Evolving | Sensitive | 13 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, NuoC, two [FeS] proteins, seven MRP antiporter subunits, etc. |
WP_011839350.1 | Staphylothermus marinus | [NiFe] Group 4b | Crenarchaeota | Desulfurococcales | Evolving | Sensitive | 13 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, NuoC, two [FeS] proteins, seven MRP antiporter subunits, etc. |
WP_013467422.1 | Thermococcus barophilus | [NiFe] Group 4b | Euryarchaeota | Thermococcales | Evolving | Sensitive | 13 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, NuoC, two [FeS] proteins, seven MRP antiporter subunits, etc. |
WP_015857865.1 | Thermococcus gammatolerans | [NiFe] Group 4b | Euryarchaeota | Thermococcales | Evolving | Sensitive | 13 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, NuoC, two [FeS] proteins, seven MRP antiporter subunits, etc. |
WP_015857684.1 | Thermococcus gammatolerans | [NiFe] Group 4b | Euryarchaeota | Thermococcales | Evolving | Sensitive | 13 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, NuoC, two [FeS] proteins, seven MRP antiporter subunits, etc. |
WP_004068373.1 | Thermococcus litoralis | [NiFe] Group 4b | Euryarchaeota | Thermococcales | Evolving | Sensitive | 13 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, NuoC, two [FeS] proteins, seven MRP antiporter subunits, etc. |
WP_012571234.1 | Thermococcus onnurineus | [NiFe] Group 4b | Euryarchaeota | Thermococcales | Evolving | Sensitive | 13 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, NuoC, two [FeS] proteins, seven MRP antiporter subunits, etc. |
WP_012571983.1 | Thermococcus onnurineus | [NiFe] Group 4b | Euryarchaeota | Thermococcales | Evolving | Sensitive | 13 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, NuoC, two [FeS] proteins, seven MRP antiporter subunits, etc. |
WP_012572531.1 | Thermococcus onnurineus | [NiFe] Group 4b | Euryarchaeota | Thermococcales | Evolving | Sensitive | 13 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, NuoC, two [FeS] proteins, seven MRP antiporter subunits, etc. |
WP_014012072.1 | Thermococcus sp. 4557 | [NiFe] Group 4b | Euryarchaeota | Thermococcales | Evolving | Sensitive | 13 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, NuoC, two [FeS] proteins, seven MRP antiporter subunits, etc. |
WP_014123101.1 | Thermococcus sp. AM4 | [NiFe] Group 4b | Euryarchaeota | Thermococcales | Evolving | Sensitive | 13 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, NuoC, two [FeS] proteins, seven MRP antiporter subunits, etc. |
WP_014122941.1 | Thermococcus sp. AM4 | [NiFe] Group 4b | Euryarchaeota | Thermococcales | Evolving | Sensitive | 13 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, NuoC, two [FeS] proteins, seven MRP antiporter subunits, etc. |
WP_028842781.1 | Thermodesulfovibrio islandicus | [NiFe] Group 4b | Nitrospirae | Nitrospirales | Evolving | Sensitive | 13 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, NuoC, two [FeS] proteins, seven MRP antiporter subunits, etc. |
WP_028845048.1 | Thermodesulfovibrio thiophilus | [NiFe] Group 4b | Nitrospirae | Nitrospirales | Evolving | Sensitive | 13 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, NuoC, two [FeS] proteins, seven MRP antiporter subunits, etc. |
WP_012545774.1 | Thermodesulfovibrio yellowstonii | [NiFe] Group 4b | Nitrospirae | Nitrospirales | Evolving | Sensitive | 13 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, NuoC, two [FeS] proteins, seven MRP antiporter subunits, etc. |
WP_014737671.1 | Thermogladius cellulolyticus | [NiFe] Group 4b | Crenarchaeota | Desulfurococcales | Evolving | Sensitive | 13 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, NuoC, two [FeS] proteins, seven MRP antiporter subunits, etc. |
WP_013128928.1 | Thermosphaera aggregans | [NiFe] Group 4b | Crenarchaeota | Desulfurococcales | Evolving | Sensitive | 13 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, NuoC, two [FeS] proteins, seven MRP antiporter subunits, etc. |
Genetics:
Physiology: