This entry was last updated at: Feb. 9, 2018, 7:09 a.m.
Group | [NiFe] Group 4: Respiratory H2-evolving [NiFe] hydrogenases |
Subgroup | [NiFe] Group 4a: Formate hydrogenlyases |
Function | Directly couples formate oxidation to H2 evolution. It remains unclear whether this process can be coupled to proton-translocation through the transmembrane modules. Reverse reaction occurs in vitro but unresolved whether it occurs in vivo. |
Activity | H2-evolution |
Oxygen tolerance | O2-sensitive |
Localisation | Membrane-bound |
Ecosystem distribution | Animal gastrointestinal tracts |
Taxonomic distribution | Encoded in Enterobacteria and several other Proteobacteria |
Organisation 1: Escherichia coli hyc operon (WP_014639275.1):
Organisation 2: Escherichia coli hyf operon (WP_014641051.1):
Structures |
|
Subunits | 6 or 9 |
Subunit description | HycE / HyfG (hydrogenase large subunit) HycG / HyfI (hydrogenase small subunit) HycF / HyfH (iron-sulfur protein) HycB / HyfA (iron-sulfur protein) HycC / HyfB (NuoL-like transmembrane adaptors) HycD / HyfC (NuoH-like transmembrane adaptors) Hyf-type enzymes additionally associated with three antiporter-like subunits (HyfD, HyfE, HyfF) Loosely associated with formate dehydrogenase module (FdhF) |
Catalytic site | [NiFe]-centre |
FeS clusters | Proximal: 4Cys[4Fe4S] Medial: Absent Distal: Absent |
NCBI Accession | Organism | Hydrogenase Class | Phylum | Order | Activity (Predicted) | Oxygen Tolerance (Predicted) | Subunits (Predicted) | Metal Centres (Predicted) | Accessory Subunits (Predicted) |
---|---|---|---|---|---|---|---|---|---|
WP_020454964.1 | Enterobacter sp. R4-368 | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_008499575.1 | Enterobacter sp. SST3 | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_016310471.1 | Enterorhabdus caecimuris | [NiFe] Group 4a | Actinobacteria | Coriobacterales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_028027490.1 | Enterorhabdus mucosicola | [NiFe] Group 4a | Actinobacteria | Coriobacterales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_027712521.1 | Erwinia chrysanthemi | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_017802632.1 | Erwinia toletana | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_002461475.1 | Escherichia albertii | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_014639275.1 | Escherichia coli | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_014641051.1 | Escherichia coli | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_001102339.1 | Escherichia fergusonii | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_002436255.1 | Escherichia hermannii | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_014558423.1 | Fervidicoccus fontis | [NiFe] Group 4a | Crenarchaeota | Fervidicoccales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_013400781.1 | Geobacillus sp. Y4.1MC1 | [NiFe] Group 4a | Firmicutes | Bacillales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_013876883.1 | Geobacillus thermoglucosidasius | [NiFe] Group 4a | Firmicutes | Bacillales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_005645455.1 | Haemophilus haemolyticus | [NiFe] Group 4a | Gammaproteobacteria | Pasteurellales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_014065500.1 | Haemophilus parainfluenzae | [NiFe] Group 4a | Gammaproteobacteria | Pasteurellales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_007242420.1 | Haemophilus pittmaniae | [NiFe] Group 4a | Gammaproteobacteria | Pasteurellales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_025797024.1 | Hafnia alvei | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_025801577.1 | Hafnia alvei | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_004114746.1 | Klebsiella oxytoca | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_023342092.1 | Klebsiella pneumoniae | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_022065323.1 | Klebsiella variicola | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_007370867.1 | Kosakonia radicincitans | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_007370513.1 | Kosakonia radicincitans | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_027275601.1 | Leminorella grimontii | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_025773400.1 | Moorella thermoacetica | [NiFe] Group 4a | Firmicutes | Thermoanaerobacterales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_025151932.1 | Morganella morganii | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_028536385.1 | Paludibacterium yongneupense | [NiFe] Group 4a | Betaproteobacteria | Neisseriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_013511773.1 | Pantoea sp. At-9b | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_005760524.1 | Pasteurella bettyae | [NiFe] Group 4a | Gammaproteobacteria | Pasteurellales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_005764546.1 | Pasteurella dagmatis | [NiFe] Group 4a | Gammaproteobacteria | Pasteurellales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_011092831.1 | Pectobacterium atrosepticum | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_005366168.1 | Photobacterium angustum | [NiFe] Group 4a | Gammaproteobacteria | Vibrionales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_008989674.1 | Photobacterium leiognathi | [NiFe] Group 4a | Gammaproteobacteria | Vibrionales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_006232936.1 | Photobacterium profundum | [NiFe] Group 4a | Gammaproteobacteria | Vibrionales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_007469705.1 | Photobacterium sp. AK15 | [NiFe] Group 4a | Gammaproteobacteria | Vibrionales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_006644813.1 | Photobacterium sp. SKA34 | [NiFe] Group 4a | Gammaproteobacteria | Vibrionales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_004245689.1 | Proteus mirabilis | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_006536160.1 | Proteus penneri | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_006658446.1 | Providencia alcalifaciens | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_008912486.1 | Providencia burhodogranariea | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_006814426.1 | Providencia rustigianii | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_008916657.1 | Providencia sneebia | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_015466266.1 | Psychromonas sp. CNPT3 | [NiFe] Group 4a | Gammaproteobacteria | Alteromonadales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_015696362.1 | Rahnella aquatilis | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_013574483.1 | Rahnella sp. Y9602 | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_011474970.1 | Rhodopseudomonas palustris | [NiFe] Group 4a | Alphaproteobacteria | Rhizobiales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_008384313.1 | Rhodovulum sp. PH10 | [NiFe] Group 4a | Alphaproteobacteria | Rhodobacterales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_020845056.1 | Salmonella bongori | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_023227550.1 | Salmonella enterica | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
Genetics:
Physiology:
Biochemistry: