This entry was last updated at: Feb. 9, 2018, 7:09 a.m.
Group | [NiFe] Group 4: Respiratory H2-evolving [NiFe] hydrogenases |
Subgroup | [NiFe] Group 4a: Formate hydrogenlyases |
Function | Directly couples formate oxidation to H2 evolution. It remains unclear whether this process can be coupled to proton-translocation through the transmembrane modules. Reverse reaction occurs in vitro but unresolved whether it occurs in vivo. |
Activity | H2-evolution |
Oxygen tolerance | O2-sensitive |
Localisation | Membrane-bound |
Ecosystem distribution | Animal gastrointestinal tracts |
Taxonomic distribution | Encoded in Enterobacteria and several other Proteobacteria |
Organisation 1: Escherichia coli hyc operon (WP_014639275.1):
Organisation 2: Escherichia coli hyf operon (WP_014641051.1):
Structures |
|
Subunits | 6 or 9 |
Subunit description | HycE / HyfG (hydrogenase large subunit) HycG / HyfI (hydrogenase small subunit) HycF / HyfH (iron-sulfur protein) HycB / HyfA (iron-sulfur protein) HycC / HyfB (NuoL-like transmembrane adaptors) HycD / HyfC (NuoH-like transmembrane adaptors) Hyf-type enzymes additionally associated with three antiporter-like subunits (HyfD, HyfE, HyfF) Loosely associated with formate dehydrogenase module (FdhF) |
Catalytic site | [NiFe]-centre |
FeS clusters | Proximal: 4Cys[4Fe4S] Medial: Absent Distal: Absent |
NCBI Accession | Organism | Hydrogenase Class | Phylum | Order | Activity (Predicted) | Oxygen Tolerance (Predicted) | Subunits (Predicted) | Metal Centres (Predicted) | Accessory Subunits (Predicted) |
---|---|---|---|---|---|---|---|---|---|
WP_002445316.1 | Shimwellia blattae | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_002440707.1 | Shimwellia blattae | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_004699424.1 | Yersinia aldovae | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_005273806.1 | Yersinia bercovieri | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_005162305.1 | Yersinia enterocolitica | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_004709176.1 | Yersinia frederiksenii | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_005187619.1 | Yersinia intermedia | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_004388743.1 | Yersinia kristensenii | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_004877798.1 | Yersinia mollaretii | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_004713500.1 | Yersinia rohdei | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_004720656.1 | Yersinia ruckeri | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_006818557.1 | Yokenella regensburgei | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_014558423.1 | Fervidicoccus fontis | [NiFe] Group 4a | Crenarchaeota | Fervidicoccales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_007473606.1 | Caminibacter mediatlanticus | [NiFe] Group 4a | Epsilonproteobacteria | Nautiliales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_028536385.1 | Paludibacterium yongneupense | [NiFe] Group 4a | Betaproteobacteria | Neisseriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_005542188.1 | Aggregatibacter actinomycetemcomitans | [NiFe] Group 4a | Gammaproteobacteria | Pasteurellales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_005703984.1 | Aggregatibacter aphrophilus | [NiFe] Group 4a | Gammaproteobacteria | Pasteurellales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_005645455.1 | Haemophilus haemolyticus | [NiFe] Group 4a | Gammaproteobacteria | Pasteurellales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_014065500.1 | Haemophilus parainfluenzae | [NiFe] Group 4a | Gammaproteobacteria | Pasteurellales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_007242420.1 | Haemophilus pittmaniae | [NiFe] Group 4a | Gammaproteobacteria | Pasteurellales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_005760524.1 | Pasteurella bettyae | [NiFe] Group 4a | Gammaproteobacteria | Pasteurellales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_005764546.1 | Pasteurella dagmatis | [NiFe] Group 4a | Gammaproteobacteria | Pasteurellales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_011474970.1 | Rhodopseudomonas palustris | [NiFe] Group 4a | Alphaproteobacteria | Rhizobiales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_008384313.1 | Rhodovulum sp. PH10 | [NiFe] Group 4a | Alphaproteobacteria | Rhodobacterales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_018609272.1 | Uliginosibacterium gangwonense | [NiFe] Group 4a | Betaproteobacteria | Rhodocyclales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_029010114.1 | Azospirillum halopraeferens | [NiFe] Group 4a | Alphaproteobacteria | Rhodospirillales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_014249328.1 | Azospirillum lipoferum | [NiFe] Group 4a | Alphaproteobacteria | Rhodospirillales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_022587809.1 | Caldanaerobacter subterraneus | [NiFe] Group 4a | Firmicutes | Thermoanaerobacterales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_025773400.1 | Moorella thermoacetica | [NiFe] Group 4a | Firmicutes | Thermoanaerobacterales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_015051661.1 | Thermacetogenium phaeum | [NiFe] Group 4a | Firmicutes | Thermoanaerobacterales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_011751858.1 | Thermofilum pendens | [NiFe] Group 4a | Crenarchaeota | Thermoproteales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_005366168.1 | Photobacterium angustum | [NiFe] Group 4a | Gammaproteobacteria | Vibrionales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_008989674.1 | Photobacterium leiognathi | [NiFe] Group 4a | Gammaproteobacteria | Vibrionales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_006232936.1 | Photobacterium profundum | [NiFe] Group 4a | Gammaproteobacteria | Vibrionales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_007469705.1 | Photobacterium sp. AK15 | [NiFe] Group 4a | Gammaproteobacteria | Vibrionales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_006644813.1 | Photobacterium sp. SKA34 | [NiFe] Group 4a | Gammaproteobacteria | Vibrionales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_004726128.1 | Vibrio furnissii | [NiFe] Group 4a | Gammaproteobacteria | Vibrionales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_021019734.1 | Vibrio gazogenes | [NiFe] Group 4a | Gammaproteobacteria | Vibrionales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
Genetics:
Physiology:
Biochemistry: