This entry was last updated at: Feb. 9, 2018, 7:09 a.m.
Group | [NiFe] Group 4: Respiratory H2-evolving [NiFe] hydrogenases |
Subgroup | [NiFe] Group 4a: Formate hydrogenlyases |
Function | Directly couples formate oxidation to H2 evolution. It remains unclear whether this process can be coupled to proton-translocation through the transmembrane modules. Reverse reaction occurs in vitro but unresolved whether it occurs in vivo. |
Activity | H2-evolution |
Oxygen tolerance | O2-sensitive |
Localisation | Membrane-bound |
Ecosystem distribution | Animal gastrointestinal tracts |
Taxonomic distribution | Encoded in Enterobacteria and several other Proteobacteria |
Organisation 1: Escherichia coli hyc operon (WP_014639275.1):
Organisation 2: Escherichia coli hyf operon (WP_014641051.1):
Structures |
|
Subunits | 6 or 9 |
Subunit description | HycE / HyfG (hydrogenase large subunit) HycG / HyfI (hydrogenase small subunit) HycF / HyfH (iron-sulfur protein) HycB / HyfA (iron-sulfur protein) HycC / HyfB (NuoL-like transmembrane adaptors) HycD / HyfC (NuoH-like transmembrane adaptors) Hyf-type enzymes additionally associated with three antiporter-like subunits (HyfD, HyfE, HyfF) Loosely associated with formate dehydrogenase module (FdhF) |
Catalytic site | [NiFe]-centre |
FeS clusters | Proximal: 4Cys[4Fe4S] Medial: Absent Distal: Absent |
NCBI Accession | Organism | Hydrogenase Class | Phylum | Order | Activity (Predicted) | Oxygen Tolerance (Predicted) | Subunits (Predicted) | Metal Centres (Predicted) | Accessory Subunits (Predicted) |
---|---|---|---|---|---|---|---|---|---|
WP_017781337.1 | Aeromonas hydrophila | [NiFe] Group 4a | Gammaproteobacteria | Aeromonadales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_005315165.1 | Aeromonas salmonicida | [NiFe] Group 4a | Gammaproteobacteria | Aeromonadales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_019839258.1 | Aeromonas sp. MDS8 | [NiFe] Group 4a | Gammaproteobacteria | Aeromonadales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_005333319.1 | Aeromonas veronii | [NiFe] Group 4a | Gammaproteobacteria | Aeromonadales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_015466266.1 | Psychromonas sp. CNPT3 | [NiFe] Group 4a | Gammaproteobacteria | Alteromonadales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_025820124.1 | Shewanella marina | [NiFe] Group 4a | Gammaproteobacteria | Alteromonadales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_026823431.1 | Arsenophonus nasoniae | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_009113583.1 | Brenneria sp. EniD312 | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_029095340.1 | Budvicia aquatica | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_016538796.1 | Cedecea davisae | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_012134832.1 | Citrobacter koseri | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_012907207.1 | Citrobacter rodentium | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_006687169.1 | Citrobacter youngae | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_007725151.1 | Cronobacter dublinensis | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_007792874.1 | Cronobacter malonaticus | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_024559362.1 | Cronobacter pulveris | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_012124960.1 | Cronobacter sakazakii | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_015741042.1 | Cronobacter turicensis | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_013317233.1 | Dickeya dadantii | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_024105256.1 | Dickeya dianthicola | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_022632933.1 | Dickeya solani | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_019844844.1 | Dickeya zeae | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_024524460.1 | Edwardsiella hoshinae | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_015872494.1 | Edwardsiella ictaluri | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_005281999.1 | Edwardsiella tarda | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_015370028.1 | Enterobacter aerogenes | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_029739528.1 | Enterobacter asburiae | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_006178188.1 | Enterobacter cancerogenus | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_020883834.1 | Enterobacter cloacae | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_006811737.1 | Enterobacter hormaechei | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_013365010.1 | Enterobacter lignolyticus | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_010434474.1 | Enterobacter mori | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_025912384.1 | Enterobacter sp. 5-4 | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_008457682.1 | Enterobacter sp. Ag1 | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_023331166.1 | Enterobacter sp. MGH 25 | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_020454964.1 | Enterobacter sp. R4-368 | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_008499575.1 | Enterobacter sp. SST3 | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_027712521.1 | Erwinia chrysanthemi | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_017802632.1 | Erwinia toletana | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_002461475.1 | Escherichia albertii | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_014639275.1 | Escherichia coli | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_014641051.1 | Escherichia coli | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_001102339.1 | Escherichia fergusonii | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_002436255.1 | Escherichia hermannii | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_025797024.1 | Hafnia alvei | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_025801577.1 | Hafnia alvei | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_004114746.1 | Klebsiella oxytoca | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_023342092.1 | Klebsiella pneumoniae | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_022065323.1 | Klebsiella variicola | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
WP_007370867.1 | Kosakonia radicincitans | [NiFe] Group 4a | Gammaproteobacteria | Enterobacteriales | Evolving | Sensitive | 6 or 9 | [NiFe]-centre, 1 x [4Fe4S] clusters | NuoL, NuoH, two [FeS] proteins, (three antiporter subunits) |
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Physiology:
Biochemistry: