This entry was last updated at: Feb. 9, 2018, 6:37 a.m.
Group | [NiFe] Group 3: Cofactor-coupled bidirectional [NiFe] hydrogenases |
Subgroup | [NiFe] Group 3d: NAD-coupled |
Function | Generates reductant for hydrogenotrophic carbon-fixation by directly coupling oxidation of H2 to reduction of NAD. Also acts in reverse direction to catalyse fermentative NADH-dependent production of H2. Electrons flow between H2-activating hydrogenase subunits to NAD-activating diaphorase subunits. |
Activity | Bidirectional |
Oxygen tolerance | O2-tolerant |
Localisation | Cytosolic |
Ecosystem distribution | Diverse soil and aquatic environments |
Taxonomic distribution | Encoded in diverse Proteobacteria and Cyanobacteria |
e.g. Ralstonia eutropha (WP_011154013.1):
Structures | |
Subunits | 4 or 5 |
Subunit description | HoxH (hydrogenase large subunit) HoxY (hydrogenase small subunit) HoxF (diaphorase catalytic subunit) HoxU (diaphorase electron transfer subunit) Photosynthetic bacteria also contain HoxE (iron-sulfur subunit) |
Catalytic site | [NiFe]-centre |
FeS clusters | Proximal: 4Cys[4Fe4S] Medial: Absent Distal: Absent |
NCBI Accession | Organism | Hydrogenase Class | Phylum | Order | Activity (Predicted) | Oxygen Tolerance (Predicted) | Subunits (Predicted) | Metal Centres (Predicted) | Accessory Subunits (Predicted) |
---|---|---|---|---|---|---|---|---|---|
WP_028085013.1 | Dolichospermum circinale | [NiFe] Group 3d | Cyanobacteria | Nostocales | Bidirectional | Tolerant | 4 | [NiFe]-centre, 1 x [4Fe4S] clusters | Diaphorase, [FeS] protein |
WP_028088931.1 | Dolichospermum circinale | [NiFe] Group 3d | Cyanobacteria | Nostocales | Bidirectional | Tolerant | 4 | [NiFe]-centre, 1 x [4Fe4S] clusters | Diaphorase, [FeS] protein |
WP_006195766.1 | Nodularia spumigena | [NiFe] Group 3d | Cyanobacteria | Nostocales | Bidirectional | Tolerant | 4 | [NiFe]-centre, 1 x [4Fe4S] clusters | Diaphorase, [FeS] protein |
WP_015113185.1 | Nostoc sp. PCC 7107 | [NiFe] Group 3d | Cyanobacteria | Nostocales | Bidirectional | Tolerant | 4 | [NiFe]-centre, 1 x [4Fe4S] clusters | Diaphorase, [FeS] protein |
WP_010994940.1 | Nostoc sp. PCC 7120 | [NiFe] Group 3d | Cyanobacteria | Nostocales | Bidirectional | Tolerant | 4 | [NiFe]-centre, 1 x [4Fe4S] clusters | Diaphorase, [FeS] protein |
WP_015138057.1 | Nostoc sp. PCC 7524 | [NiFe] Group 3d | Cyanobacteria | Nostocales | Bidirectional | Tolerant | 4 | [NiFe]-centre, 1 x [4Fe4S] clusters | Diaphorase, [FeS] protein |
WP_009341606.1 | Raphidiopsis brookii | [NiFe] Group 3d | Cyanobacteria | Nostocales | Bidirectional | Tolerant | 4 | [NiFe]-centre, 1 x [4Fe4S] clusters | Diaphorase, [FeS] protein |
WP_011394103.1 | Hahella chejuensis | [NiFe] Group 3d | Gammaproteobacteria | Oceanospirillales | Bidirectional | Tolerant | 4 | [NiFe]-centre, 1 x [4Fe4S] clusters | Diaphorase, [FeS] protein |
WP_020410523.1 | Hahella ganghwensis | [NiFe] Group 3d | Gammaproteobacteria | Oceanospirillales | Bidirectional | Tolerant | 4 | [NiFe]-centre, 1 x [4Fe4S] clusters | Diaphorase, [FeS] protein |
WP_006669190.1 | Arthrospira maxima | [NiFe] Group 3d | Cyanobacteria | Oscillatoriales | Bidirectional | Tolerant | 4 | [NiFe]-centre, 1 x [4Fe4S] clusters | Diaphorase, [FeS] protein |
WP_006624081.1 | Arthrospira platensis | [NiFe] Group 3d | Cyanobacteria | Oscillatoriales | Bidirectional | Tolerant | 4 | [NiFe]-centre, 1 x [4Fe4S] clusters | Diaphorase, [FeS] protein |
WP_008053057.1 | Arthrospira sp. PCC 8005 | [NiFe] Group 3d | Cyanobacteria | Oscillatoriales | Bidirectional | Tolerant | 4 | [NiFe]-centre, 1 x [4Fe4S] clusters | Diaphorase, [FeS] protein |
WP_006101686.1 | Coleofasciculus chthonoplastes | [NiFe] Group 3d | Cyanobacteria | Oscillatoriales | Bidirectional | Tolerant | 4 | [NiFe]-centre, 1 x [4Fe4S] clusters | Diaphorase, [FeS] protein |
WP_013323481.1 | Cyanothece sp. PCC 7822 | [NiFe] Group 3d | Cyanobacteria | Oscillatoriales | Bidirectional | Tolerant | 4 | [NiFe]-centre, 1 x [4Fe4S] clusters | Diaphorase, [FeS] protein |
WP_017659307.1 | Geitlerinema sp. PCC 7105 | [NiFe] Group 3d | Cyanobacteria | Oscillatoriales | Bidirectional | Tolerant | 4 | [NiFe]-centre, 1 x [4Fe4S] clusters | Diaphorase, [FeS] protein |
WP_015171978.1 | Geitlerinema sp. PCC 7407 | [NiFe] Group 3d | Cyanobacteria | Oscillatoriales | Bidirectional | Tolerant | 4 | [NiFe]-centre, 1 x [4Fe4S] clusters | Diaphorase, [FeS] protein |
WP_006512920.1 | Leptolyngbya sp. PCC 7375 | [NiFe] Group 3d | Cyanobacteria | Oscillatoriales | Bidirectional | Tolerant | 4 | [NiFe]-centre, 1 x [4Fe4S] clusters | Diaphorase, [FeS] protein |
WP_023067990.1 | Lyngbya aestuarii | [NiFe] Group 3d | Cyanobacteria | Oscillatoriales | Bidirectional | Tolerant | 4 | [NiFe]-centre, 1 x [4Fe4S] clusters | Diaphorase, [FeS] protein |
WP_009785505.1 | Lyngbya sp. PCC 8106 | [NiFe] Group 3d | Cyanobacteria | Oscillatoriales | Bidirectional | Tolerant | 4 | [NiFe]-centre, 1 x [4Fe4S] clusters | Diaphorase, [FeS] protein |
WP_015182972.1 | Microcoleus sp. PCC 7113 | [NiFe] Group 3d | Cyanobacteria | Oscillatoriales | Bidirectional | Tolerant | 4 | [NiFe]-centre, 1 x [4Fe4S] clusters | Diaphorase, [FeS] protein |
WP_015151979.1 | Oscillatoria acuminata | [NiFe] Group 3d | Cyanobacteria | Oscillatoriales | Bidirectional | Tolerant | 4 | [NiFe]-centre, 1 x [4Fe4S] clusters | Diaphorase, [FeS] protein |
WP_015175286.1 | Oscillatoria nigro-viridis | [NiFe] Group 3d | Cyanobacteria | Oscillatoriales | Bidirectional | Tolerant | 4 | [NiFe]-centre, 1 x [4Fe4S] clusters | Diaphorase, [FeS] protein |
WP_017715405.1 | Oscillatoria sp. PCC 10802 | [NiFe] Group 3d | Cyanobacteria | Oscillatoriales | Bidirectional | Tolerant | 4 | [NiFe]-centre, 1 x [4Fe4S] clusters | Diaphorase, [FeS] protein |
WP_019498425.1 | Pseudanabaena sp. PCC 6802 | [NiFe] Group 3d | Cyanobacteria | Oscillatoriales | Bidirectional | Tolerant | 4 | [NiFe]-centre, 1 x [4Fe4S] clusters | Diaphorase, [FeS] protein |
WP_017305034.1 | Spirulina subsalsa | [NiFe] Group 3d | Cyanobacteria | Oscillatoriales | Bidirectional | Tolerant | 4 | [NiFe]-centre, 1 x [4Fe4S] clusters | Diaphorase, [FeS] protein |
WP_015153897.1 | Chroococcidiopsis thermalis | [NiFe] Group 3d | Cyanobacteria | Pleurocapsales | Bidirectional | Tolerant | 4 | [NiFe]-centre, 1 x [4Fe4S] clusters | Diaphorase, [FeS] protein |
WP_015144267.1 | Pleurocapsa minor | [NiFe] Group 3d | Cyanobacteria | Pleurocapsales | Bidirectional | Tolerant | 4 | [NiFe]-centre, 1 x [4Fe4S] clusters | Diaphorase, [FeS] protein |
WP_019509061.1 | Pleurocapsa sp. PCC 7319 | [NiFe] Group 3d | Cyanobacteria | Pleurocapsales | Bidirectional | Tolerant | 4 | [NiFe]-centre, 1 x [4Fe4S] clusters | Diaphorase, [FeS] protein |
WP_006512117.1 | Xenococcus sp. PCC 7305 | [NiFe] Group 3d | Cyanobacteria | Pleurocapsales | Bidirectional | Tolerant | 4 | [NiFe]-centre, 1 x [4Fe4S] clusters | Diaphorase, [FeS] protein |
WP_012591738.1 | Methylocella silvestris | [NiFe] Group 3d | Alphaproteobacteria | Rhizobiales | Bidirectional | Tolerant | 4 | [NiFe]-centre, 1 x [4Fe4S] clusters | Diaphorase, [FeS] protein |
WP_020174963.1 | Methyloferula stellata | [NiFe] Group 3d | Alphaproteobacteria | Rhizobiales | Bidirectional | Tolerant | 4 | [NiFe]-centre, 1 x [4Fe4S] clusters | Diaphorase, [FeS] protein |
WP_006931831.1 | Labrenzia aggregata | [NiFe] Group 3d | Alphaproteobacteria | Rhodobacterales | Bidirectional | Tolerant | 4 | [NiFe]-centre, 1 x [4Fe4S] clusters | Diaphorase, [FeS] protein |
WP_008193041.1 | Labrenzia alexandrii | [NiFe] Group 3d | Alphaproteobacteria | Rhodobacterales | Bidirectional | Tolerant | 4 | [NiFe]-centre, 1 x [4Fe4S] clusters | Diaphorase, [FeS] protein |
WP_008384205.1 | Rhodovulum sp. PH10 | [NiFe] Group 3d | Alphaproteobacteria | Rhodobacterales | Bidirectional | Tolerant | 4 | [NiFe]-centre, 1 x [4Fe4S] clusters | Diaphorase, [FeS] protein |
WP_011765148.1 | Azoarcus sp. BH72 | [NiFe] Group 3d | Betaproteobacteria | Rhodocyclales | Bidirectional | Tolerant | 4 | [NiFe]-centre, 1 x [4Fe4S] clusters | Diaphorase, [FeS] protein |
WP_015436644.1 | Azoarcus sp. KH32C | [NiFe] Group 3d | Betaproteobacteria | Rhodocyclales | Bidirectional | Tolerant | 4 | [NiFe]-centre, 1 x [4Fe4S] clusters | Diaphorase, [FeS] protein |
WP_018992236.1 | Azoarcus toluclasticus | [NiFe] Group 3d | Betaproteobacteria | Rhodocyclales | Bidirectional | Tolerant | 4 | [NiFe]-centre, 1 x [4Fe4S] clusters | Diaphorase, [FeS] protein |
WP_028994701.1 | Azonexus hydrophilus | [NiFe] Group 3d | Betaproteobacteria | Rhodocyclales | Bidirectional | Tolerant | 4 | [NiFe]-centre, 1 x [4Fe4S] clusters | Diaphorase, [FeS] protein |
WP_014238042.1 | Azospira oryzae | [NiFe] Group 3d | Betaproteobacteria | Rhodocyclales | Bidirectional | Tolerant | 4 | [NiFe]-centre, 1 x [4Fe4S] clusters | Diaphorase, [FeS] protein |
WP_026687097.1 | Azovibrio restrictus | [NiFe] Group 3d | Betaproteobacteria | Rhodocyclales | Bidirectional | Tolerant | 4 | [NiFe]-centre, 1 x [4Fe4S] clusters | Diaphorase, [FeS] protein |
WP_027457859.1 | Dechloromonas agitata | [NiFe] Group 3d | Betaproteobacteria | Rhodocyclales | Bidirectional | Tolerant | 4 | [NiFe]-centre, 1 x [4Fe4S] clusters | Diaphorase, [FeS] protein |
WP_018412521.1 | Methyloversatilis sp. NVD | [NiFe] Group 3d | Betaproteobacteria | Rhodocyclales | Bidirectional | Tolerant | 4 | [NiFe]-centre, 1 x [4Fe4S] clusters | Diaphorase, [FeS] protein |
WP_020162845.1 | Methyloversatilis universalis | [NiFe] Group 3d | Betaproteobacteria | Rhodocyclales | Bidirectional | Tolerant | 4 | [NiFe]-centre, 1 x [4Fe4S] clusters | Diaphorase, [FeS] protein |
WP_004322153.1 | Thauera aminoaromatica | [NiFe] Group 3d | Betaproteobacteria | Rhodocyclales | Bidirectional | Tolerant | 4 | [NiFe]-centre, 1 x [4Fe4S] clusters | Diaphorase, [FeS] protein |
WP_004363405.1 | Thauera phenylacetica | [NiFe] Group 3d | Betaproteobacteria | Rhodocyclales | Bidirectional | Tolerant | 4 | [NiFe]-centre, 1 x [4Fe4S] clusters | Diaphorase, [FeS] protein |
WP_002937457.1 | Thauera sp. 27 | [NiFe] Group 3d | Betaproteobacteria | Rhodocyclales | Bidirectional | Tolerant | 4 | [NiFe]-centre, 1 x [4Fe4S] clusters | Diaphorase, [FeS] protein |
WP_002929027.1 | Thauera sp. 28 | [NiFe] Group 3d | Betaproteobacteria | Rhodocyclales | Bidirectional | Tolerant | 4 | [NiFe]-centre, 1 x [4Fe4S] clusters | Diaphorase, [FeS] protein |
WP_004252763.1 | Thauera sp. 63 | [NiFe] Group 3d | Betaproteobacteria | Rhodocyclales | Bidirectional | Tolerant | 4 | [NiFe]-centre, 1 x [4Fe4S] clusters | Diaphorase, [FeS] protein |
WP_012585448.1 | Thauera sp. MZ1T | [NiFe] Group 3d | Betaproteobacteria | Rhodocyclales | Bidirectional | Tolerant | 4 | [NiFe]-centre, 1 x [4Fe4S] clusters | Diaphorase, [FeS] protein |
WP_011385756.1 | Magnetospirillum magneticum | [NiFe] Group 3d | Alphaproteobacteria | Rhodospirillales | Bidirectional | Tolerant | 4 | [NiFe]-centre, 1 x [4Fe4S] clusters | Diaphorase, [FeS] protein |
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