This entry was last updated at: June 13, 2016, 11:23 a.m.
Group | [NiFe] Group 3: Cofactor-coupled bidirectional [NiFe] hydrogenases |
Subgroup | [NiFe] Group 3c: Heterodisulfide reductase-linked |
Function | Bifurcates electrons from H2 to heterodisulfide and ferredoxin. This is the final step in the Wolfe cycle of methanogenesis. |
Activity | Electron-bifurcation |
Oxygen tolerance | O2-tolerant? |
Localisation | Cytosolic |
Ecosystem distribution | Methanogenic environments such as anoxic soils, marine sediments, and gastrointestinal tracts |
Taxonomic distribution | Encoded in most methanogens and sulfate-reducing bacteria |
e.g. Methanothermobacter marburgensis (WP_013296307.1):
Structures |
|
Subunits | 4 |
Subunit description | MvhA (hydrogenase large subunit) MvhG (hydrogenase small subunit) MvhD (iron-sulfur subunit) MvhB (polyferredoxin subunit) Forms complex with heterodisulfide reductase (HdrABC) |
Catalytic site | [NiFe]-centre or [NiFeSe]-centre |
FeS clusters | Proximal: 4Cys[4Fe4S] Medial: 4Cys[4Fe4S] Distal: 4Cys[4Fe4S] |
NCBI Accession | Organism | Hydrogenase Class | Phylum | Order | Activity (Predicted) | Oxygen Tolerance (Predicted) | Subunits (Predicted) | Metal Centres (Predicted) | Accessory Subunits (Predicted) |
---|---|---|---|---|---|---|---|---|---|
WP_012528247.1 | Anaeromyxobacter sp. K | [NiFe] Group 3c | Deltaproteobacteria | Myxococcales | Bifurcating | Sensitive | 4 | [NiFe]-centre, 3 x [4Fe4S] clusters | Polyferredoxin, [FeS] protein |
WP_011700738.1 | Syntrophobacter fumaroxidans | [NiFe] Group 3c | Deltaproteobacteria | Syntrophobacterales | Bifurcating | Sensitive | 4 | [NiFe]-centre, 3 x [4Fe4S] clusters | Polyferredoxin, [FeS] protein |
WP_011698648.1 | Syntrophobacter fumaroxidans | [NiFe] Group 3c | Deltaproteobacteria | Syntrophobacterales | Bifurcating | Sensitive | 4 | [NiFe]-centre, 3 x [4Fe4S] clusters | Polyferredoxin, [FeS] protein |
WP_011699072.1 | Syntrophobacter fumaroxidans | [NiFe] Group 3c | Deltaproteobacteria | Syntrophobacterales | Bifurcating | Sensitive | 4 | [NiFe]-centre, 3 x [4Fe4S] clusters | Polyferredoxin, [FeS] protein |
WP_011700332.1 | Syntrophobacter fumaroxidans | [NiFe] Group 3c | Deltaproteobacteria | Syntrophobacterales | Bifurcating | Sensitive | 4 | [NiFe]-centre, 3 x [4Fe4S] clusters | Polyferredoxin, [FeS] protein |
WP_028894885.1 | Syntrophorhabdus aromaticivorans | [NiFe] Group 3c | Deltaproteobacteria | Syntrophobacterales | Bifurcating | Sensitive | 4 | [NiFe]-centre, 3 x [4Fe4S] clusters | Polyferredoxin, [FeS] protein |
WP_027358538.1 | Desulforegula conservatrix | [NiFe] Group 3c | Deltaproteobacteria | Desulfuromonadales | Bidirectional | Tolerant | 4 | [NiFe]-centre, 1 x [4Fe4S] clusters | Diaphorase, [FeS] protein |
WP_012532312.1 | Geobacter bemidjiensis | [NiFe] Group 3c | Deltaproteobacteria | Desulfuromonadales | Bidirectional | Tolerant | 4 | [NiFe]-centre, 1 x [4Fe4S] clusters | Diaphorase, [FeS] protein |
WP_012469611.1 | Geobacter lovleyi | [NiFe] Group 3c | Deltaproteobacteria | Desulfuromonadales | Bidirectional | Tolerant | 4 | [NiFe]-centre, 1 x [4Fe4S] clusters | Diaphorase, [FeS] protein |
WP_004512544.1 | Geobacter metallireducens | [NiFe] Group 3c | Deltaproteobacteria | Desulfuromonadales | Bidirectional | Tolerant | 4 | [NiFe]-centre, 1 x [4Fe4S] clusters | Diaphorase, [FeS] protein |
WP_015839165.1 | Geobacter sp. M21 | [NiFe] Group 3c | Deltaproteobacteria | Desulfuromonadales | Bidirectional | Tolerant | 4 | [NiFe]-centre, 1 x [4Fe4S] clusters | Diaphorase, [FeS] protein |
WP_014551809.1 | Geobacter sulfurreducens | [NiFe] Group 3c | Deltaproteobacteria | Desulfuromonadales | Bidirectional | Tolerant | 4 | [NiFe]-centre, 1 x [4Fe4S] clusters | Diaphorase, [FeS] protein |
WP_010936390.1 | Dehalococcoides mccartyi | [NiFe] Group 3c | Chloroflexi | Dehalococcoidales | Bifurcating | Sensitive | 4 | [NiFe]-centre, 3 x [4Fe4S] clusters | Polyferredoxin, [FeS] protein |
WP_013218178.1 | Dehalogenimonas lykanthroporepellens | [NiFe] Group 3c | Chloroflexi | Dehalogenimonas | Bifurcating | Sensitive | 4 | [NiFe]-centre, 3 x [4Fe4S] clusters | Polyferredoxin, [FeS] protein |
WP_006930005.1 | Caldithrix abyssi | [NiFe] Group 3c | Caldithrix | Caldithrixales | Bifurcating | Sensitive | 4 | [NiFe]-centre, 3 x [4Fe4S] clusters | Polyferredoxin, [FeS] protein |
WP_022775381.1 | Symbiobacter mobilis | [NiFe] Group 3c | Betaproteobacteria | Burkholderiales | Bifurcating | Sensitive | 4 | [NiFe]-centre, 3 x [4Fe4S] clusters | Polyferredoxin, [FeS] protein |
WP_013637988.1 | Desulfurobacterium thermolithotrophum | [NiFe] Group 3c | Aquificae | Desulfurobacteriales | Bifurcating | Sensitive | 4 | [NiFe]-centre, 3 x [4Fe4S] clusters | Polyferredoxin, [FeS] protein |
Genetics:
Physiology:
Biochemistry:
Chemistry: