This entry was last updated at: June 13, 2016, 11:23 a.m.
Group | [NiFe] Group 3: Cofactor-coupled bidirectional [NiFe] hydrogenases |
Subgroup | [NiFe] Group 3c: Heterodisulfide reductase-linked |
Function | Bifurcates electrons from H2 to heterodisulfide and ferredoxin. This is the final step in the Wolfe cycle of methanogenesis. |
Activity | Electron-bifurcation |
Oxygen tolerance | O2-tolerant? |
Localisation | Cytosolic |
Ecosystem distribution | Methanogenic environments such as anoxic soils, marine sediments, and gastrointestinal tracts |
Taxonomic distribution | Encoded in most methanogens and sulfate-reducing bacteria |
e.g. Methanothermobacter marburgensis (WP_013296307.1):
Structures |
|
Subunits | 4 |
Subunit description | MvhA (hydrogenase large subunit) MvhG (hydrogenase small subunit) MvhD (iron-sulfur subunit) MvhB (polyferredoxin subunit) Forms complex with heterodisulfide reductase (HdrABC) |
Catalytic site | [NiFe]-centre or [NiFeSe]-centre |
FeS clusters | Proximal: 4Cys[4Fe4S] Medial: 4Cys[4Fe4S] Distal: 4Cys[4Fe4S] |
NCBI Accession | Organism | Hydrogenase Class | Phylum | Order | Activity (Predicted) | Oxygen Tolerance (Predicted) | Subunits (Predicted) | Metal Centres (Predicted) | Accessory Subunits (Predicted) |
---|---|---|---|---|---|---|---|---|---|
WP_011018638.1 | Methanopyrus kandleri | [NiFe] Group 3c | Euryarchaeota | Methanopyrales | Bifurcating | Sensitive | 4 | [NiFe]-centre, 3 x [4Fe4S] clusters | Polyferredoxin, [FeS] protein |
WP_011018550.1 | Methanopyrus kandleri | [NiFe] Group 3c | Euryarchaeota | Methanopyrales | Bifurcating | Sensitive | 4 | [NiFe]-centre, 3 x [4Fe4S] clusters | Polyferredoxin, [FeS] protein |
WP_010936390.1 | Dehalococcoides mccartyi | [NiFe] Group 3c | Chloroflexi | Dehalococcoidales | Bifurcating | Sensitive | 4 | [NiFe]-centre, 3 x [4Fe4S] clusters | Polyferredoxin, [FeS] protein |
WP_010878869.1 | Archaeoglobus fulgidus | [NiFe] Group 3c | Euryarchaeota | Archaeoglobales | Bifurcating | Sensitive | 4 | [NiFe]-centre, 3 x [4Fe4S] clusters | Polyferredoxin, [FeS] protein |
WP_010876758.1 | Methanothermobacter thermoautotrophicus | [NiFe] Group 3c | Euryarchaeota | Methanobacteriales | Bifurcating | Sensitive | 4 | [NiFe]-centre, 3 x [4Fe4S] clusters | Polyferredoxin, [FeS] protein |
WP_010870705.1 | Methanocaldococcus jannaschii | [NiFe] Group 3c | Euryarchaeota | Methanococcales | Bifurcating | Sensitive | 4 | [NiFe]-centre, 3 x [4Fe4S] clusters | Polyferredoxin, [FeS] protein |
WP_008870928.1 | Desulfonatronospira thiodismutans | [NiFe] Group 3c | Deltaproteobacteria | Desulfovibrionales | Bifurcating | Sensitive | 4 | [NiFe]-centre, 3 x [4Fe4S] clusters | Polyferredoxin, [FeS] protein |
WP_008869690.1 | Desulfonatronospira thiodismutans | [NiFe] Group 3c | Deltaproteobacteria | Desulfovibrionales | Bifurcating | Sensitive | 4 | [NiFe]-centre, 3 x [4Fe4S] clusters | Polyferredoxin, [FeS] protein |
WP_007044701.1 | Methanotorris formicicus | [NiFe] Group 3c | Euryarchaeota | Methanococcales | Bifurcating | Sensitive | 4 | [NiFe]-centre, 3 x [4Fe4S] clusters | Polyferredoxin, [FeS] protein |
WP_007043614.1 | Methanotorris formicicus | [NiFe] Group 3c | Euryarchaeota | Methanococcales | Bifurcating | Sensitive | 4 | [NiFe]-centre, 3 x [4Fe4S] clusters | Polyferredoxin, [FeS] protein |
WP_006930005.1 | Caldithrix abyssi | [NiFe] Group 3c | Caldithrix | Caldithrixales | Bifurcating | Sensitive | 4 | [NiFe]-centre, 3 x [4Fe4S] clusters | Polyferredoxin, [FeS] protein |
WP_004589824.1 | Methanocaldococcus villosus | [NiFe] Group 3c | Euryarchaeota | Methanococcales | Bifurcating | Sensitive | 4 | [NiFe]-centre, 3 x [4Fe4S] clusters | Polyferredoxin, [FeS] protein |
WP_004512544.1 | Geobacter metallireducens | [NiFe] Group 3c | Deltaproteobacteria | Desulfuromonadales | Bidirectional | Tolerant | 4 | [NiFe]-centre, 1 x [4Fe4S] clusters | Diaphorase, [FeS] protein |
WP_004039570.1 | Methanofollis liminatans | [NiFe] Group 3c | Euryarchaeota | Methanomicrobiales | Bifurcating | Sensitive | 4 | [NiFe]-centre, 3 x [4Fe4S] clusters | Polyferredoxin, [FeS] protein |
WP_004032005.1 | Methanobacterium formicicum | [NiFe] Group 3c | Euryarchaeota | Methanobacteriales | Bifurcating | Sensitive | 4 | [NiFe]-centre, 3 x [4Fe4S] clusters | Polyferredoxin, [FeS] protein |
WP_004031311.1 | Methanobacterium formicicum | [NiFe] Group 3c | Euryarchaeota | Methanobacteriales | Bifurcating | Sensitive | 4 | [NiFe]-centre, 3 x [4Fe4S] clusters | Polyferredoxin, [FeS] protein |
KKK40681.1 | Lokiarchaeum sp. GC14_75 | [NiFe] Group 3c | Lokiarchaeota | N/A | Bifurcating | Sensitive | 4 | [NiFe]-centre, 3 x [4Fe4S] clusters | Polyferredoxin, [FeS] protein |
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