[NiFe] Group 3b-hydrogenase

This entry was last updated at: Feb. 9, 2018, 7:34 a.m.

Properties
Group [NiFe] Group 3: Cofactor-coupled bidirectional [NiFe] hydrogenases
Subgroup [NiFe] Group 3b: NADP-coupled
Function Directly couples oxidation of NADPH to fermentative evolution of H2. Reverse reaction may also occur. Some complexes controversially proposed to harbour sulfhydrogenase activity.
Activity Bidirectional
Oxygen tolerance O2-tolerant
Localisation Cytosolic
Distribution
Ecosystem distribution Diverse soil and aquatic environments
Taxonomic distribution Encoded in multiple diverse bacterial and archaeal phyla
Genetic Organisation

e.g. Pyrococcus furiosus (WP_011012029.1):

Architecture
Structures
  • 5ODC (Methanothermococcus thermolithotrophicus, 2.3 Å resolution)
  • 5ODH (Methanothermococcus thermolithotrophicus, 2.2 Å resolution, bound with heterodisulfide)
  • 5ODI (Methanothermococcus thermolithotrophicus, 2.4 Å resolution, bound with coenzyme M)
Subunits 4
Subunit description HyhL (hydrogenase large subunit)
HyhS (hydrogenase small subunit)
HyhG (diaphorase catalytic subunit)
HyhB (diaphorase electron transfer subunit)
Catalytic site [NiFe]-centre
FeS clusters Proximal: 4Cys[4Fe4S]
Medial: 4Cys[4Fe4S]
Distal: 4Cys[4Fe4S]
Important Notes
Despite the ubiquitous nature of this hydrogenase, the enzyme has only been purified from Pyrococcus furiosus. It remains unclear whether bacterial enzymes are also NADP+-specific.
Sequences in this class
NCBI AccessionOrganismHydrogenase ClassPhylumOrderActivity (Predicted)Oxygen Tolerance (Predicted)Subunits (Predicted)Metal Centres (Predicted)Accessory Subunits (Predicted)
WP_018290138.1Verrucomicrobium sp. 3C[NiFe] Group 3bVerrucomicrobiaVerrucomicrobialesBidirectionalTolerant4[NiFe]-centre, 3 x [4Fe4S] clustersDiaphorase, [FeS] protein
WP_048090768.1Methanoperedens nitroreducens[NiFe] Group 3bEuryarchaeotaMethanosarcinalesBidirectionalTolerant4[NiFe]-centre, 3 x [4Fe4S] clustersDiaphorase, [FeS] protein
WP_048088262.1Methanoperedens nitroreducens[NiFe] Group 3bEuryarchaeotaMethanosarcinalesBidirectionalTolerant4[NiFe]-centre, 3 x [4Fe4S] clustersDiaphorase, [FeS] protein
CUU06124.1Kryptonium thompsoni[NiFe] Group 3bKryptoniaN/ABidirectionalTolerant4[NiFe]-centre, 3 x [4Fe4S] clustersDiaphorase, [FeS] protein

Literature

Genetics:

Physiology:

Biochemistry:

Chemistry: