[NiFe] Group 1j-hydrogenase
This entry was last updated at: Feb. 9, 2018, 6:22 a.m.
Properties
| Group | [NiFe] Group 1: Respiratory H2-uptake [NiFe] hydrogenases |
| Subgroup | [NiFe] Group 1j: Archaeoglobi-type |
| Function | Hydrogenotrophic respiration using sulfate, iron, or nitrate as terminal electron acceptors. Enzyme transfers H2-liberated electrons through cyt b and quinone to terminal reductase. |
| Activity | H2-uptake (unidirectional) |
| Oxygen tolerance | O2-sensitive |
| Localisation | Membrane-bound |
Distribution
| Ecosystem distribution | Hydrothermal systems, other high temperature environments |
| Taxonomic distribution | Exclusive to Euryarchaeota of the order Archaeoglobales |
Genetic Organisation
e.g. Archaeoglobus fulgidus (WP_010878877.1):
Architecture
| Structures |
|
| Subunits | 3? |
| Subunit description | VhzA (hydrogenase large subunit) VhzG (hydrogenase small subunit) VhzC (cytochrome b subunit) |
| Catalytic site | NiFe]-centre |
| FeS clusters | Proximal: 4Cys[4Fe4S] Medial: 4Cys[4Fe4S] Distal: 3Cys1Asp[4Fe4S] or 3Cys1Asn[4Fe4S] |
Important Notes
This hydrogenase class has yet to be studied genetically or biochemically. Its properties are inferred from genomic predictions and systematic bacteriology studies.
Sequences in this class
| NCBI Accession | Organism | Hydrogenase Class | Phylum | Order | Activity (Predicted) | Oxygen Tolerance (Predicted) | Subunits (Predicted) | Metal Centres (Predicted) | Accessory Subunits (Predicted) |
|---|---|---|---|---|---|---|---|---|---|
| WP_010878877.1 | Archaeoglobus fulgidus | [NiFe] Group 1j | Euryarchaeota | Archaeoglobales | Anaerobic Uptake | Sensitive | 3 | [NiFe]-centre, 3 x [4Fe4S] clusters | Cytochrome b |
| WP_012940402.1 | Archaeoglobus profundus | [NiFe] Group 1j | Euryarchaeota | Archaeoglobales | Anaerobic Uptake | Sensitive | 3 | [NiFe]-centre, 3 x [4Fe4S] clusters | Cytochrome b |
| WP_015591799.1 | Archaeoglobus sulfaticallidus | [NiFe] Group 1j | Euryarchaeota | Archaeoglobales | Anaerobic Uptake | Sensitive | 3 | [NiFe]-centre, 3 x [4Fe4S] clusters | Cytochrome b |
| WP_013682857.1 | Archaeoglobus veneficus | [NiFe] Group 1j | Euryarchaeota | Archaeoglobales | Anaerobic Uptake | Sensitive | 3 | [NiFe]-centre, 3 x [4Fe4S] clusters | Cytochrome b |
| WP_012965344.1 | Ferroglobus placidus | [NiFe] Group 1j | Euryarchaeota | Archaeoglobales | Anaerobic Uptake | Sensitive | 3 | [NiFe]-centre, 3 x [4Fe4S] clusters | Cytochrome b |
Literature
Genetics:
- Hocking, W.P., Stokke, R., Roalkvam, I., and Steen, I.H. (2014) Identification of key components in the energy metabolism of the hyperthermophilic sulfate-reducing archaeon Archaeoglobus fulgidus by transcriptome analyses. Front. Microbiol. 5: 95.
- Søndergaard, D., Petersen, C.N.S., and Greening, C. (2017) HydDB: a web tool for hydrogenase classification and analysis. Sci. Rep. 6: 34212.
Physiology:
- Kashefi, K., Tor, J.M., Holmes, D.E., Gaw Van Praagh, C.V., Reysenbach, A.-L., and Lovley, D.R. (2002) Geoglobus ahangari gen. nov., sp. nov., a novel hyperthermophilic archaeon capable of oxidizing organic acids and growing autotrophically on hydrogen with Fe(III) serving as the sole electron acceptor. Int. J. Syst. Evol. Microbiol. 52:719-728.
- Stetter, K.O. (1988) Archaeoglobus fulgidus gen. nov., sp. nov.: a new taxon of extremely thermophilic archaebacteria. Syst. Appl. Microbiol. 10: 172-173.
- Vorholt, J.A., Hafenbradl, D., Stetter, K.O., and Thauer, R.K. (1997) Pathways of autotrophic CO2 fixation and of dissimilatory nitrate reduction to N2O in Ferroglobus placidus. Arch. Microbiol. 167: 19-23.