This entry was last updated at: Feb. 9, 2018, 3:51 a.m.
Group | [NiFe] Group 1: Respiratory H2-uptake [NiFe] hydrogenases |
Subgroup | [NiFe] Group 1h: Actinobacteria-type |
Function | Hydrogenotrophic respiration using O2 as terminal electron acceptor. Enzyme scavenges electrons from atmospheric H2 to fuel respiratory chain during carbon-starvation. Route of electron transfer unresolved. |
Activity | H2-uptake (unidirectional, high-affinity) |
Oxygen tolerance | O2-tolerant or O2-insensitive |
Localisation | Membrane-associated? |
Ecosystem distribution | Upland soils, plant tissues, possibly surface waters |
Taxonomic distribution | Widespread among obligately aerobic soil bacteria, especially Actinobacteria, Acidobacteria, and Chloroflexi |
e.g. Mycobacterium smegmatis (WP_003893634.1):
Structures |
|
Subunits | 3? |
Subunit description | HyhL (hydrogenase large subunit) HyhS (hydrogenase small subunit) HyhE (putative iron-sulfur protein and proposed physiological electron acceptor) |
Catalytic site | [NiFe]-centre |
FeS clusters | Proximal: 3Cys1Asp[4Fe4S] Medial: 4Cys[4Fe4S] Distal: 3Cys1His[4Fe4S] |
NCBI Accession | Organism | Hydrogenase Class | Phylum | Order | Activity (Predicted) | Oxygen Tolerance (Predicted) | Subunits (Predicted) | Metal Centres (Predicted) | Accessory Subunits (Predicted) |
---|---|---|---|---|---|---|---|---|---|
WP_012890477.1 | Streptosporangium roseum | [NiFe] Group 1h | Actinobacteria | Actinomycetales | Aerobic Uptake | Tolerant | 3 | [NiFe]-centre, 3 x [4Fe4S] clusters | [FeS] protein |
WP_013132859.1 | Thermobispora bispora | [NiFe] Group 1h | Actinobacteria | Actinomycetales | Aerobic Uptake | Tolerant | 3 | [NiFe]-centre, 3 x [4Fe4S] clusters | [FeS] protein |
WP_012854897.1 | Thermomonospora curvata | [NiFe] Group 1h | Actinobacteria | Actinomycetales | Aerobic Uptake | Tolerant | 3 | [NiFe]-centre, 3 x [4Fe4S] clusters | [FeS] protein |
WP_014267363.1 | Granulicella mallensis | [NiFe] Group 1h | Acidobacteria | Acidobacteriales | Aerobic Uptake | Tolerant | 3 | [NiFe]-centre, 3 x [4Fe4S] clusters | [FeS] protein |
Genetics:
Physiology:
Biochemistry: