[NiFe] Group 1c-hydrogenase

This entry was last updated at: March 2, 2017, 1:49 a.m.

Properties
Group [NiFe] Group 1: Respiratory H2-uptake [NiFe] hydrogenases
Subgroup [NiFe] Group 1c: Hyb-type
Function Hydrogenotrophic respiration using fumarate, sulfate, or metals as terminal electron acceptors. Route of electron transfer unresolved. Can also fermentatively evolve H2 under hypoxia.
Activity H2-uptake (unidirectional)
Oxygen tolerance O2-sensitive
Localisation Membrane-bound
Distribution
Ecosystem distribution Animal gastrointestinal tracts, anoxic soils and sediments
Taxonomic distribution Widespread in Enterobacteria and various sulfate-reducing bacteria
Genetic Organisation

e.g. Escherichia coli (WP_021568771.1):

Architecture
Structures
    None
Subunits 4
Subunit description HybC (hydrogenase large subunit)
HybO (hydrogenase small subunit)
HybA (iron-sulfur protein and proposed physiological electron acceptor)
HybB (transmembrane protein of unresolved function)
Catalytic site [NiFe]-centre
FeS clusters Proximal: 4Cys[4Fe4S]
Medial: 3Cys[3Fe4S]
Distal: 3Cys1His[4Fe4S]
Sequences in this class
NCBI AccessionOrganismHydrogenase ClassPhylumOrderActivity (Predicted)Oxygen Tolerance (Predicted)Subunits (Predicted)Metal Centres (Predicted)Accessory Subunits (Predicted)
WP_011688559.1Solibacter usitatus[NiFe] Group 1cAcidobacteriaSolibacteralesAnaerobic UptakeSensitive4[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster[FeS] protein, TM subunit
WP_011714122.1Magnetococcus marinus[NiFe] Group 1cAlphaproteobacteriaMagnetococcalesAnaerobic UptakeSensitive4[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster[FeS] protein, TM subunit
WP_011938839.1Geobacter uraniireducens[NiFe] Group 1cDeltaproteobacteriaDesulfuromonadalesAnaerobic UptakeSensitive4[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster[FeS] protein, TM subunit
WP_012073017.1Actinobacillus succinogenes[NiFe] Group 1cGammaproteobacteriaEnterobacterialesAnaerobic UptakeSensitive4[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster[FeS] protein, TM subunit
WP_012098324.1Anaeromyxobacter sp. Fw109-5[NiFe] Group 1cDeltaproteobacteriaMyxococcalesAnaerobic UptakeSensitive4[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster[FeS] protein, TM subunit
WP_012135127.1Citrobacter koseri[NiFe] Group 1cGammaproteobacteriaEnterobacterialesAnaerobic UptakeSensitive4[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster[FeS] protein, TM subunit
WP_012468242.1Geobacter lovleyi[NiFe] Group 1cDeltaproteobacteriaDesulfuromonadalesAnaerobic UptakeSensitive4[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster[FeS] protein, TM subunit
WP_012524582.1Anaeromyxobacter sp. K[NiFe] Group 1cDeltaproteobacteriaMyxococcalesAnaerobic UptakeSensitive4[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster[FeS] protein, TM subunit
WP_012771488.1Aggregatibacter aphrophilus[NiFe] Group 1cGammaproteobacteriaPasteurellalesAnaerobic UptakeSensitive4[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster[FeS] protein, TM subunit
WP_012774796.1Geobacter sp. M21[NiFe] Group 1cDeltaproteobacteriaDesulfuromonadalesAnaerobic UptakeSensitive4[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster[FeS] protein, TM subunit
WP_012907588.1Citrobacter rodentium[NiFe] Group 1cGammaproteobacteriaEnterobacterialesAnaerobic UptakeSensitive4[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster[FeS] protein, TM subunit
WP_013010514.1Denitrovibrio acetiphilus[NiFe] Group 1cDeferribacteresDeferribacteralesAnaerobic UptakeSensitive4[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster[FeS] protein, TM subunit
WP_013318689.1Dickeya dadantii[NiFe] Group 1cGammaproteobacteriaEnterobacterialesAnaerobic UptakeSensitive4[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster[FeS] protein, TM subunit
WP_013366452.1Enterobacter lignolyticus[NiFe] Group 1cGammaproteobacteriaEnterobacterialesAnaerobic UptakeSensitive4[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster[FeS] protein, TM subunit
WP_013537874.1Thermovibrio ammonificans[NiFe] Group 1cAquificaeDesulfurobacterialesAnaerobic UptakeSensitive4[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster[FeS] protein, TM subunit
WP_013638066.1Desulfurobacterium thermolithotrophum[NiFe] Group 1cAquificaeDesulfurobacterialesAnaerobic UptakeSensitive4[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster[FeS] protein, TM subunit
WP_013650389.1Yersinia enterocolitica[NiFe] Group 1cGammaproteobacteriaEnterobacterialesAnaerobic UptakeSensitive4[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster[FeS] protein, TM subunit
WP_013886108.1Flexistipes sinusarabici[NiFe] Group 1cDeferribacteresDeferribacteralesAnaerobic UptakeSensitive4[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster[FeS] protein, TM subunit
WP_013906919.1Thermodesulfatator indicus[NiFe] Group 1cThermodesulfobacteriaThermodesulfobacterialesAnaerobic UptakeSensitive4[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster[FeS] protein, TM subunit
WP_013909681.1Thermodesulfobacterium geofontis[NiFe] Group 1cThermodesulfobacteriaThermodesulfobacterialesAnaerobic UptakeSensitive4[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster[FeS] protein, TM subunit
WP_014064532.1Haemophilus parainfluenzae[NiFe] Group 1cGammaproteobacteriaPasteurellalesAnaerobic UptakeSensitive4[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster[FeS] protein, TM subunit
WP_014198378.1Azospirillum brasilense[NiFe] Group 1cAlphaproteobacteriaRhodospirillalesAnaerobic UptakeSensitive4[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster[FeS] protein, TM subunit
WP_014238024.1Azospira oryzae[NiFe] Group 1cBetaproteobacteriaRhodocyclalesAnaerobic UptakeSensitive4[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster[FeS] protein, TM subunit
WP_014780162.1Thiocystis violascens[NiFe] Group 1cGammaproteobacteriaChromatialesAnaerobic UptakeSensitive4[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster[FeS] protein, TM subunit
WP_015403419.1Desulfocapsa sulfexigens[NiFe] Group 1cDeltaproteobacteriaDesulfobacteralesAnaerobic UptakeSensitive4[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster[FeS] protein, TM subunit
WP_015437386.1Azoarcus sp. KH32C[NiFe] Group 1cBetaproteobacteriaRhodocyclalesAnaerobic UptakeSensitive4[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster[FeS] protein, TM subunit
WP_015721386.1Geobacter sp. M18[NiFe] Group 1cDeltaproteobacteriaDesulfuromonadalesAnaerobic UptakeSensitive4[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster[FeS] protein, TM subunit
WP_015725977.1Desulfobulbus propionicus[NiFe] Group 1cDeltaproteobacteriaDesulfobacteralesAnaerobic UptakeSensitive4[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster[FeS] protein, TM subunit
WP_015766767.1Accumulibacter phosphatis[NiFe] Group 1cBetaproteobacteriaUnclassifiedAnaerobic UptakeSensitive4[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster[FeS] protein, TM subunit
WP_015871932.1Edwardsiella ictaluri[NiFe] Group 1cGammaproteobacteriaEnterobacterialesAnaerobic UptakeSensitive4[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster[FeS] protein, TM subunit
WP_016941064.1Dickeya zeae[NiFe] Group 1cGammaproteobacteriaEnterobacterialesAnaerobic UptakeSensitive4[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster[FeS] protein, TM subunit
WP_017778886.1Aeromonas hydrophila[NiFe] Group 1cGammaproteobacteriaAeromonadalesAnaerobic UptakeSensitive4[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster[FeS] protein, TM subunit
WP_021230237.1Aeromonas veronii[NiFe] Group 1cGammaproteobacteriaAeromonadalesAnaerobic UptakeSensitive4[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster[FeS] protein, TM subunit
WP_021568771.1Escherichia coli[NiFe] Group 1cGammaproteobacteriaEnterobacterialesAnaerobic UptakeSensitive4[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster[FeS] protein, TM subunit
WP_022634172.1Dickeya solani[NiFe] Group 1cGammaproteobacteriaEnterobacterialesAnaerobic UptakeSensitive4[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster[FeS] protein, TM subunit
WP_022846122.1Desulfurobacterium sp. TC5-1[NiFe] Group 1cAquificaeDesulfurobacterialesAnaerobic UptakeSensitive4[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster[FeS] protein, TM subunit
WP_022851983.1Geovibrio sp. L21-Ace-BES[NiFe] Group 1cDeferribacteresDeferribacteralesAnaerobic UptakeSensitive4[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster[FeS] protein, TM subunit
WP_022854121.1Thermodesulfatator atlanticus[NiFe] Group 1cThermodesulfobacteriaThermodesulfobacterialesAnaerobic UptakeSensitive4[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster[FeS] protein, TM subunit
WP_023228387.1Salmonella enterica[NiFe] Group 1cGammaproteobacteriaEnterobacterialesAnaerobic UptakeSensitive4[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster[FeS] protein, TM subunit
WP_023408348.1Desulfofustis sp. PB-SRB1[NiFe] Group 1cDeltaproteobacteriaDesulfobacteralesAnaerobic UptakeSensitive4[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster[FeS] protein, TM subunit
WP_023580917.1Proteus hauseri[NiFe] Group 1cGammaproteobacteriaEnterobacterialesAnaerobic UptakeSensitive4[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster[FeS] protein, TM subunit
WP_024080419.1Magnetospirillum gryphiswaldense[NiFe] Group 1cAlphaproteobacteriaRhodospirillalesAnaerobic UptakeSensitive4[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster[FeS] protein, TM subunit
WP_024106579.1Dickeya dianthicola[NiFe] Group 1cGammaproteobacteriaEnterobacterialesAnaerobic UptakeSensitive4[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster[FeS] protein, TM subunit
WP_024522506.1Edwardsiella hoshinae[NiFe] Group 1cGammaproteobacteriaEnterobacterialesAnaerobic UptakeSensitive4[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster[FeS] protein, TM subunit
WP_024528473.1Serratia fonticola[NiFe] Group 1cGammaproteobacteriaAeromonadalesAnaerobic UptakeSensitive4[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster[FeS] protein, TM subunit
WP_026199142.1Lamprocystis purpurea[NiFe] Group 1cGammaproteobacteriaChromatialesAnaerobic UptakeSensitive4[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster[FeS] protein, TM subunit
WP_027275754.1Leminorella grimontii[NiFe] Group 1cGammaproteobacteriaEnterobacterialesAnaerobic UptakeSensitive4[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster[FeS] protein, TM subunit
WP_027371077.1Desulfovermiculus halophilus[NiFe] Group 1cDeltaproteobacteriaDesulfovibrionalesAnaerobic UptakeSensitive4[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster[FeS] protein, TM subunit
WP_027457220.1Dechloromonas agitata[NiFe] Group 1cBetaproteobacteriaRhodocyclalesAnaerobic UptakeSensitive4[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster[FeS] protein, TM subunit
WP_027711563.1Erwinia chrysanthemi[NiFe] Group 1cGammaproteobacteriaEnterobacterialesAnaerobic UptakeSensitive4[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] cluster[FeS] protein, TM subunit

Literature

Genetics:

Physiology:

Biochemistry: