[FeFe] Group B-hydrogenase Putative
This entry was last updated at: Feb. 9, 2018, 6:17 a.m.
Properties
| Group | [FeFe]-hydrogenases |
| Subgroup | [FeFe] Group B: Colonic-type |
| Function | Unconfirmed role. Indirect evidence suggests the hydrogenase is involved in hydrogenogenic fermentation. It may couple reoxidation of ferredoxinred to the evolution of H2. |
| Activity | H2-evolution? |
| Oxygen tolerance | O2-labile |
| Localisation | Cytosolic |
Distribution
| Ecosystem distribution | Gastrointestinal tracts, anoxic soils and sediments |
| Taxonomic distribution | Widespread in colonic Bacteroides, Clostridia, and Actinobacteria |
Genetic Organisation
Organisation 1: Clostridium pasteurianum (WP_023976023.1):
Organisation 2: Clostridium carboxyidovorans (WP_007061531.1):
Architecture
| Structures |
|
| Subunits | 1 |
| Subunit description | HydM (catalytic subunit) |
| Catalytic site | [FeFe]-centre |
| FeS clusters | Organisation 1: 2 [4Fe4S] clusters Organisation 2: 3 [4Fe4S] clusters, 1 [2Fe2S] cluste |
Sequences in this class
| NCBI Accession | Organism | Hydrogenase Class | Phylum | Order | Activity (Predicted) | Oxygen Tolerance (Predicted) | Subunits (Predicted) | Metal Centres (Predicted) | Accessory Subunits (Predicted) |
|---|---|---|---|---|---|---|---|---|---|
| WP_005487149.1 | Halanaerobium saccharolyticum | [FeFe] Group B | Firmicutes | Halanaerobiales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_012635168.1 | Halothermothrix orenii | [FeFe] Group B | Firmicutes | Halanaerobiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_012635236.1 | Halothermothrix orenii | [FeFe] Group B | Firmicutes | Halanaerobiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_014553742.1 | Halanaerobium praevalens | [FeFe] Group B | Firmicutes | Halanaerobiales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_014553922.1 | Halanaerobium praevalens | [FeFe] Group B | Firmicutes | Halanaerobiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_015325789.1 | Halobacteroides halobius | [FeFe] Group B | Firmicutes | Halanaerobiales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_015326565.1 | Halobacteroides halobius | [FeFe] Group B | Firmicutes | Halanaerobiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_018247956.1 | Orenia marismortui | [FeFe] Group B | Firmicutes | Halanaerobiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_018248337.1 | Orenia marismortui | [FeFe] Group B | Firmicutes | Halanaerobiales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_012862886.1 | Sebaldella termitidis | [FeFe] Group B | Fusobacteria | Fusobacteriales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_013387868.1 | Ilyobacter polytrophus | [FeFe] Group B | Fusobacteria | Fusobacteriales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_004610270.1 | Erysipelatoclostridium spiroforme | [FeFe] Group B | Firmicutes | Erysipelotrichales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_004800637.1 | Erysipelatoclostridium dolichum | [FeFe] Group B | Firmicutes | Erysipelotrichales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_019893070.1 | Allobaculum stercoricanis | [FeFe] Group B | Firmicutes | Erysipelotrichales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_027090514.1 | Erysipelatoclostridium saccharogumia | [FeFe] Group B | Firmicutes | Erysipelotrichales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_028044437.1 | Stoquefichus massiliensis | [FeFe] Group B | Firmicutes | Erysipelotrichales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_012414920.1 | Elusimicrobium minutum | [FeFe] Group B | Elusimicrobia | Elusimicrobiales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_006009278.1 | Desulfovibrio piger | [FeFe] Group B | Deltaproteobacteria | Desulfovibrionales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_009380768.1 | Bilophila sp. 4_1_30 | [FeFe] Group B | Deltaproteobacteria | Desulfovibrionales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_011366806.1 | Desulfovibrio alaskensis | [FeFe] Group B | Deltaproteobacteria | Desulfovibrionales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_011189620.1 | Desulfotalea psychrophila | [FeFe] Group B | Deltaproteobacteria | Desulfobacterales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_003148188.1 | Atopobium rimae | [FeFe] Group B | Actinobacteria | Coriobacteriales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_009141594.1 | Collinsella tanakaei | [FeFe] Group B | Actinobacteria | Coriobacteriales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_009277946.1 | Olsenella sp. 809 | [FeFe] Group B | Actinobacteria | Coriobacteriales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_012808500.1 | Atopobium parvulum | [FeFe] Group B | Actinobacteria | Coriobacteriales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_013252233.1 | Olsenella uli | [FeFe] Group B | Actinobacteria | Coriobacteriales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_013708979.1 | Coriobacterium glomerans | [FeFe] Group B | Actinobacteria | Coriobacteriales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_021626398.1 | Atopobium sp. 810 | [FeFe] Group B | Actinobacteria | Coriobacteriales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_021724994.1 | Olsenella profusa | [FeFe] Group B | Actinobacteria | Coriobacteriales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_028263961.1 | Atopobium fossor | [FeFe] Group B | Actinobacteria | Coriobacteriales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_002568888.1 | Lachnoclostridium bolteae | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_002570587.1 | Lachnoclostridium bolteae | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_002580023.1 | Clostridium butyricum | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_002582769.1 | Clostridium butyricum | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_002843191.1 | Peptostreptococcus anaerobius | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_002845087.1 | Peptostreptococcus anaerobius | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_002848990.1 | Ruminococcus albus | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_003492333.1 | Clostridium sporogenes | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_003495774.1 | Clostridium sporogenes | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_003530568.1 | Ruminiclostridium leptum | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_003532170.1 | Ruminiclostridium leptum | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_003540297.1 | Desulfotomaculum nigrificans | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_004052440.1 | Eubacterium plexicaudatum | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_004054251.1 | Eubacterium plexicaudatum | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_004602330.1 | Eubacterium cellulosolvens | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_004605002.1 | Lachnoclostridium scindens | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_004611414.1 | Tyzzerella nexilis | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_004613839.1 | Tyzzerella nexilis | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_004627876.1 | Ruminiclostridium termitidis | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_004817153.1 | Anaerococcus hydrogenalis | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
Literature
Genetics:
- Calusinska, M., Happe, T., Joris, B., and Wilmotte, A. (2010) The surprising diversity of clostridial hydrogenases: a comparative genomic perspective. Microbiol. 156: 1575-1588.
- Greening, C., Biswas, A., Carere, C.R., Jackson, C.J., Taylor, M.C., Stott, M.B., Cook, G.M., and Morales, S.E. (2016) Genomic and metagenomic surveys of hydrogenase distribution indicate H2 is a widely utilised energy source for microbial growth and survival. ISME J. 10: 761-777.
- Wolf, P.G., Biswas, A., Morales, S.E., Greening, C., and Gaskins, H.R. (2016) H2 metabolism is widespread and diverse among human colonic microbes. Gut Microb. 7: 235-245.
Physiology:
- Betian, H.G., Linehan, B.A., Bryant, M.P., and Holdeman, L.V. (1977) Isolation of a cellulolytic Bacteroides sp. from human feces. Appl. Environ. Microbiol. 33: 1009-1010.
- Fischbach, M.A. and Sonnenburg, J.L. (2011) Eating for two: how metabolism establishes interspecies interactions in the gut. Cell Host Microb. 10: 336-347.