[FeFe] Group B-hydrogenase Putative
This entry was last updated at: Feb. 9, 2018, 6:17 a.m.
Properties
| Group | [FeFe]-hydrogenases |
| Subgroup | [FeFe] Group B: Colonic-type |
| Function | Unconfirmed role. Indirect evidence suggests the hydrogenase is involved in hydrogenogenic fermentation. It may couple reoxidation of ferredoxinred to the evolution of H2. |
| Activity | H2-evolution? |
| Oxygen tolerance | O2-labile |
| Localisation | Cytosolic |
Distribution
| Ecosystem distribution | Gastrointestinal tracts, anoxic soils and sediments |
| Taxonomic distribution | Widespread in colonic Bacteroides, Clostridia, and Actinobacteria |
Genetic Organisation
Organisation 1: Clostridium pasteurianum (WP_023976023.1):
Organisation 2: Clostridium carboxyidovorans (WP_007061531.1):
Architecture
| Structures |
|
| Subunits | 1 |
| Subunit description | HydM (catalytic subunit) |
| Catalytic site | [FeFe]-centre |
| FeS clusters | Organisation 1: 2 [4Fe4S] clusters Organisation 2: 3 [4Fe4S] clusters, 1 [2Fe2S] cluste |
Sequences in this class
| NCBI Accession | Organism | Hydrogenase Class | Phylum | Order | Activity (Predicted) | Oxygen Tolerance (Predicted) | Subunits (Predicted) | Metal Centres (Predicted) | Accessory Subunits (Predicted) |
|---|---|---|---|---|---|---|---|---|---|
| WP_026939128.1 | Holophaga foetida | [FeFe] Group B | Acidobacteria | Holophagales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_003148188.1 | Atopobium rimae | [FeFe] Group B | Actinobacteria | Coriobacteriales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_009141594.1 | Collinsella tanakaei | [FeFe] Group B | Actinobacteria | Coriobacteriales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_009277946.1 | Olsenella sp. 809 | [FeFe] Group B | Actinobacteria | Coriobacteriales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_012808500.1 | Atopobium parvulum | [FeFe] Group B | Actinobacteria | Coriobacteriales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_013252233.1 | Olsenella uli | [FeFe] Group B | Actinobacteria | Coriobacteriales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_013708979.1 | Coriobacterium glomerans | [FeFe] Group B | Actinobacteria | Coriobacteriales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_021626398.1 | Atopobium sp. 810 | [FeFe] Group B | Actinobacteria | Coriobacteriales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_021724994.1 | Olsenella profusa | [FeFe] Group B | Actinobacteria | Coriobacteriales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_028263961.1 | Atopobium fossor | [FeFe] Group B | Actinobacteria | Coriobacteriales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_022078722.1 | Acetobacter sp. CAG:267 | [FeFe] Group B | Alphaproteobacteria | Rhodospirillales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_022187469.1 | Azospirillum sp. CAG:260 | [FeFe] Group B | Alphaproteobacteria | Rhodospirillales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_022195425.1 | Azospirillum sp. CAG:239 | [FeFe] Group B | Alphaproteobacteria | Rhodospirillales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_022418160.1 | Acidiphilium sp. CAG:727 | [FeFe] Group B | Alphaproteobacteria | Rhodospirillales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| XP_008856018.1 | Entamoeba nuttalli | [FeFe] Group B | Amoebozoa | Amoebida | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_004307174.1 | Bacteroides ovatus | [FeFe] Group B | Bacteroidetes | Bacteroidales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_004330064.1 | Alistipes putredinis | [FeFe] Group B | Bacteroidetes | Bacteroidales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_005679640.1 | Bacteroides caccae | [FeFe] Group B | Bacteroidetes | Bacteroidales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_005836589.1 | Bacteroides uniformis | [FeFe] Group B | Bacteroidetes | Bacteroidales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_005929426.1 | Bacteroides salyersiae | [FeFe] Group B | Bacteroidetes | Bacteroidales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_005941153.1 | Bacteroides massiliensis | [FeFe] Group B | Bacteroidetes | Bacteroidales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_007209903.1 | Bacteroides cellulosilyticus | [FeFe] Group B | Bacteroidetes | Bacteroidales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_007560478.1 | Bacteroides plebeius | [FeFe] Group B | Bacteroidetes | Bacteroidales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_007569731.1 | Bacteroides coprocola | [FeFe] Group B | Bacteroidetes | Bacteroidales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_007665069.1 | Bacteroides intestinalis | [FeFe] Group B | Bacteroidetes | Bacteroidales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_007748547.1 | Bacteroides finegoldii | [FeFe] Group B | Bacteroidetes | Bacteroidales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_008025884.1 | Bacteroides xylanisolvens | [FeFe] Group B | Bacteroidetes | Bacteroidales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_008152383.1 | Parabacteroides johnsonii | [FeFe] Group B | Bacteroidetes | Bacteroidales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_009126776.1 | Bacteroides fluxus | [FeFe] Group B | Bacteroidetes | Bacteroidales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_009132353.1 | Bacteroides oleiciplenus | [FeFe] Group B | Bacteroidetes | Bacteroidales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_010665374.1 | Marinilabilia salmonicolor | [FeFe] Group B | Bacteroidetes | Bacteroidales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_011964716.1 | Bacteroides vulgatus | [FeFe] Group B | Bacteroidetes | Bacteroidales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_013547705.1 | Bacteroides helcogenes | [FeFe] Group B | Bacteroidetes | Bacteroidales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_013613424.1 | Odoribacter splanchnicus | [FeFe] Group B | Bacteroidetes | Bacteroidales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_013760215.1 | Porphyromonas asaccharolytica | [FeFe] Group B | Bacteroidetes | Bacteroidales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_014299406.1 | Bacteroides fragilis | [FeFe] Group B | Bacteroidetes | Bacteroidales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_016266912.1 | Bacteroides thetaiotaomicron | [FeFe] Group B | Bacteroidetes | Bacteroidales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_018338302.1 | Butyricimonas synergistica | [FeFe] Group B | Bacteroidetes | Bacteroidales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_018357720.1 | Porphyromonas levii | [FeFe] Group B | Bacteroidetes | Bacteroidales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_021644837.1 | Bacteroides pyogenes | [FeFe] Group B | Bacteroidetes | Bacteroidales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_022278255.1 | Bacteroides coprophilus | [FeFe] Group B | Bacteroidetes | Bacteroidales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_024988140.1 | Bacteroides acidifaciens | [FeFe] Group B | Bacteroidetes | Bacteroidales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_025075971.1 | Bacteroides faecichinchillae | [FeFe] Group B | Bacteroidetes | Bacteroidales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_025864379.1 | Prolixibacter bellariivorans | [FeFe] Group B | Bacteroidetes | Bacteroidales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_025868115.1 | Bacteroides nordii | [FeFe] Group B | Bacteroidetes | Bacteroidales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_027202830.1 | Butyricimonas virosa | [FeFe] Group B | Bacteroidetes | Bacteroidales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_028726954.1 | Parabacteroides gordonii | [FeFe] Group B | Bacteroidetes | Bacteroidales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_006929576.1 | Caldithrix abyssi | [FeFe] Group B | Caldithrix | Caldithrixales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_006009278.1 | Desulfovibrio piger | [FeFe] Group B | Deltaproteobacteria | Desulfovibrionales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_009380768.1 | Bilophila sp. 4_1_30 | [FeFe] Group B | Deltaproteobacteria | Desulfovibrionales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
Literature
Genetics:
- Calusinska, M., Happe, T., Joris, B., and Wilmotte, A. (2010) The surprising diversity of clostridial hydrogenases: a comparative genomic perspective. Microbiol. 156: 1575-1588.
- Greening, C., Biswas, A., Carere, C.R., Jackson, C.J., Taylor, M.C., Stott, M.B., Cook, G.M., and Morales, S.E. (2016) Genomic and metagenomic surveys of hydrogenase distribution indicate H2 is a widely utilised energy source for microbial growth and survival. ISME J. 10: 761-777.
- Wolf, P.G., Biswas, A., Morales, S.E., Greening, C., and Gaskins, H.R. (2016) H2 metabolism is widespread and diverse among human colonic microbes. Gut Microb. 7: 235-245.
Physiology:
- Betian, H.G., Linehan, B.A., Bryant, M.P., and Holdeman, L.V. (1977) Isolation of a cellulolytic Bacteroides sp. from human feces. Appl. Environ. Microbiol. 33: 1009-1010.
- Fischbach, M.A. and Sonnenburg, J.L. (2011) Eating for two: how metabolism establishes interspecies interactions in the gut. Cell Host Microb. 10: 336-347.