[FeFe] Group A3-hydrogenase

This entry was last updated at: Feb. 9, 2018, 6:46 a.m.

Properties
Group [FeFe]-hydrogenases
Subgroup [FeFe] Group A3: Bifurcating
Function Bifurcates electrons from H2 to NAD and ferredoxin. Reverse reaction can also occur resulting in reoxidation of redox carriers concomitant with fermentative H2 evolution.
Activity Electron-bifurcation
Oxygen tolerance O2-labile
Localisation Cytosolic
Distribution
Ecosystem distribution Gastrointestinal tracts, anoxic soils and sediments
Taxonomic distribution Widespread in anaerobic bacteria, including Firmicutes, Bacteroidetes, Deltaproteobacteria, and Spirochaetes
Genetic Organisation

Organisation 1: Thermotoga maritima (WP_004081677.1):


Organisation 2: Desulfovibrio fructosovorans (WP_005990603.1):

Architecture
Structures
    None
Subunits 3 or 4
Subunit description HydA (catalytic subunit)
HydB (diaphorase subunit)
HydC (thioredoxin)
In organisation 2, an additional thioredoxin (HydD) is present
Catalytic site [FeFe]-centre
FeS clusters Organisation 1: 3 [4Fe4S] clusters, 2 [2Fe2S] clusters
Organisation 2: 3 [4Fe4S] clusters, 1 [2Fe2S] cluster
Sequences in this class
NCBI AccessionOrganismHydrogenase ClassPhylumOrderActivity (Predicted)Oxygen Tolerance (Predicted)Subunits (Predicted)Metal Centres (Predicted)Accessory Subunits (Predicted)
WP_015720177.1Geobacter sp. M18[FeFe] Group A3DeltaproteobacteriaDesulfuromonadalesBifurcatingLabile4[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin, thioredoxin
WP_013406264.1Halanaerobium hydrogeniformans[FeFe] Group A3FirmicutesHalanaerobialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_013406351.1Halanaerobium hydrogeniformans[FeFe] Group A3FirmicutesHalanaerobialesBifurcatingLabile4[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin, thioredoxin
WP_014553773.1Halanaerobium praevalens[FeFe] Group A3FirmicutesHalanaerobialesBifurcatingLabile4[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin, thioredoxin
WP_005487095.1Halanaerobium saccharolyticum[FeFe] Group A3FirmicutesHalanaerobialesBifurcatingLabile4[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin, thioredoxin
WP_005487325.1Halanaerobium saccharolyticum[FeFe] Group A3FirmicutesHalanaerobialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_015326098.1Halobacteroides halobius[FeFe] Group A3FirmicutesHalanaerobialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_015327873.1Halobacteroides halobius[FeFe] Group A3FirmicutesHalanaerobialesBifurcatingLabile4[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin, thioredoxin
WP_027338924.1Halonatronum saccharophilum[FeFe] Group A3FirmicutesHalanaerobialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_027338952.1Halonatronum saccharophilum[FeFe] Group A3FirmicutesHalanaerobialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_008824689.1Haloplasma contractile[FeFe] Group A3TenericutesHaloplasmatalesBifurcatingLabile4[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin, thioredoxin
WP_012635141.1Halothermothrix orenii[FeFe] Group A3FirmicutesHalanaerobialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 2 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_012635578.1Halothermothrix orenii[FeFe] Group A3FirmicutesHalanaerobialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_012282074.1Heliobacterium modesticaldum[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_006059213.1Holdemania filiformis[FeFe] Group A3FirmicutesErysipelotrichalesBifurcatingLabile4[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin, thioredoxin
WP_005033810.1Holophaga foetida[FeFe] Group A3AcidobacteriaHolophagalesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_005034114.1Holophaga foetida[FeFe] Group A3AcidobacteriaHolophagalesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_005038259.1Holophaga foetida[FeFe] Group A3AcidobacteriaHolophagalesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 2 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_014559401.1Ignavibacterium album[FeFe] Group A3IgnavibacteriaeIgnavibacterialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_013387085.1Ilyobacter polytrophus[FeFe] Group A3FusobacteriaFusobacterialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_013387146.1Ilyobacter polytrophus[FeFe] Group A3FusobacteriaFusobacterialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_013387669.1Ilyobacter polytrophus[FeFe] Group A3FusobacteriaFusobacterialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_007286821.1Intestinibacter bartlettii[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_005541293.1Johnsonella ignava[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_008521264.1Jonquetella anthropi[FeFe] Group A3SynergistetesSynergistalesBifurcatingLabile1[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_026892188.1Lachnoclostridium aerotolerans[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile4[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin, thioredoxin
WP_007715797.1Lachnoclostridium asparagiforme[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_002568591.1Lachnoclostridium bolteae[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_002570913.1Lachnoclostridium bolteae[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_007862869.1Lachnoclostridium citroniae[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile4[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin, thioredoxin
WP_007862872.1Lachnoclostridium citroniae[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_027642167.1Lachnoclostridium clostridioforme[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile4[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin, thioredoxin
WP_004844038.1Lachnoclostridium gnavus[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_006778965.1Lachnoclostridium hathewayi[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile4[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin, thioredoxin
WP_006442445.1Lachnoclostridium hylemonae[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile4[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin, thioredoxin
WP_006444067.1Lachnoclostridium hylemonae[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_029502586.1Lachnoclostridium phytofermentans[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_029502830.1Lachnoclostridium phytofermentans[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile4[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin, thioredoxin
WP_013273987.1Lachnoclostridium saccharolyticum[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_004608286.1Lachnoclostridium scindens[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile4[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin, thioredoxin
WP_003507872.1Lachnoclostridium symbiosum[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile4[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin, thioredoxin
WP_015528256.1Lachnoclostridium torques[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_031366038.1Lactococcus chungangensis[FeFe] Group A3FirmicutesLactobacillalesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_003138680.1Lactococcus raffinolactis[FeFe] Group A3FirmicutesLactobacillalesBifurcatingLabile3[FeFe]-centre, 2 x [4Fe4S] clustersNADH dehydrogenase, thioredoxin
WP_021767225.1Leptotrichia sp. 215[FeFe] Group A3FusobacteriaFusobacterialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_013780878.1Mahella australiensis[FeFe] Group A3FirmicutesThermoanaerobacteralesBifurcatingLabile4[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin, thioredoxin
WP_013781004.1Mahella australiensis[FeFe] Group A3FirmicutesThermoanaerobacteralesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_010663850.1Marinilabilia salmonicolor[FeFe] Group A3BacteroidetesBacteroidalesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_010665320.1Marinilabilia salmonicolor[FeFe] Group A3BacteroidetesBacteroidalesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_014296497.1Marinitoga piezophila[FeFe] Group A3ThermotogaeThermotogalesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin

Literature

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