[FeFe] Group A3-hydrogenase

This entry was last updated at: Feb. 9, 2018, 6:46 a.m.

Properties
Group [FeFe]-hydrogenases
Subgroup [FeFe] Group A3: Bifurcating
Function Bifurcates electrons from H2 to NAD and ferredoxin. Reverse reaction can also occur resulting in reoxidation of redox carriers concomitant with fermentative H2 evolution.
Activity Electron-bifurcation
Oxygen tolerance O2-labile
Localisation Cytosolic
Distribution
Ecosystem distribution Gastrointestinal tracts, anoxic soils and sediments
Taxonomic distribution Widespread in anaerobic bacteria, including Firmicutes, Bacteroidetes, Deltaproteobacteria, and Spirochaetes
Genetic Organisation

Organisation 1: Thermotoga maritima (WP_004081677.1):


Organisation 2: Desulfovibrio fructosovorans (WP_005990603.1):

Architecture
Structures
    None
Subunits 3 or 4
Subunit description HydA (catalytic subunit)
HydB (diaphorase subunit)
HydC (thioredoxin)
In organisation 2, an additional thioredoxin (HydD) is present
Catalytic site [FeFe]-centre
FeS clusters Organisation 1: 3 [4Fe4S] clusters, 2 [2Fe2S] clusters
Organisation 2: 3 [4Fe4S] clusters, 1 [2Fe2S] cluster
Sequences in this class
NCBI AccessionOrganismHydrogenase ClassPhylumOrderActivity (Predicted)Oxygen Tolerance (Predicted)Subunits (Predicted)Metal Centres (Predicted)Accessory Subunits (Predicted)
WP_013657394.1Cellulosilyticum lentocellum[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile4[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin, thioredoxin
WP_013756843.1Thermodesulfobium narugense[FeFe] Group A3FirmicutesThermoanaerobacteralesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_013757500.1Treponema brennaborense[FeFe] Group A3SpirochaetesSpirochaetalesBifurcatingLabile4[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin, thioredoxin
WP_013778939.1Tepidanaerobacter acetatoxydans[FeFe] Group A3FirmicutesThermoanaerobacteralesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_013779523.1Tepidanaerobacter acetatoxydans[FeFe] Group A3FirmicutesThermoanaerobacteralesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 2 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_013780878.1Mahella australiensis[FeFe] Group A3FirmicutesThermoanaerobacteralesBifurcatingLabile4[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin, thioredoxin
WP_013781004.1Mahella australiensis[FeFe] Group A3FirmicutesThermoanaerobacteralesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_013787823.1Thermoanaerobacterium xylanolyticum[FeFe] Group A3FirmicutesThermoanaerobacteralesBifurcatingLabile4[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin, thioredoxin
WP_013809932.1Desulfotomaculum carboxydivorans[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile3[FeFe]-centre, 2 x [4Fe4S] clustersNADH dehydrogenase, thioredoxin
WP_013810075.1Desulfotomaculum carboxydivorans[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 2 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_013810492.1Desulfotomaculum carboxydivorans[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile2[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase
WP_013823357.1Desulfotomaculum kuznetsovii[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_013824004.1Desulfotomaculum kuznetsovii[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 2 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_013840577.1Desulfotomaculum ruminis[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_013842391.1Desulfotomaculum ruminis[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 2 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_013842395.1Desulfotomaculum ruminis[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_013842583.1Desulfotomaculum ruminis[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile3[FeFe]-centre, 2 x [4Fe4S] clustersNADH dehydrogenase, thioredoxin
WP_013843380.1Desulfotomaculum ruminis[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile3[FeFe]-centre, 2 x [4Fe4S] clustersNADH dehydrogenase, thioredoxin
WP_013931427.1Thermotoga thermarum[FeFe] Group A3ThermotogaeThermotogalesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 2 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_013970147.1Treponema caldaria[FeFe] Group A3SpirochaetesSpirochaetalesBifurcatingLabile4[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin, thioredoxin
WP_014062655.1Thermoanaerobacter wiegelii[FeFe] Group A3FirmicutesThermoanaerobacteralesBifurcatingLabile4[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin, thioredoxin
WP_014117537.1Oscillibacter valericigenes[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_014184036.1Desulfosporosinus orientis[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_014187152.1Desulfosporosinus orientis[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile3[FeFe]-centre, 2 x [4Fe4S] clustersNADH dehydrogenase, thioredoxin
WP_014187542.1Desulfosporosinus orientis[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_014254579.1Ruminiclostridium clariflavum[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile4[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin, thioredoxin
WP_014271080.1Sphaerochaeta pleomorpha[FeFe] Group A3SpirochaetesSpirochaetalesBifurcatingLabile4[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin, thioredoxin
WP_014296497.1Marinitoga piezophila[FeFe] Group A3ThermotogaeThermotogalesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_014357048.1Acetobacterium woodii[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile4[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin, thioredoxin
WP_014413848.1Rhodospirillum photometricum[FeFe] Group A3AlphaproteobacteriaRhodospirillalesBifurcatingLabile2[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase
WP_014425762.1Selenomonas ruminantium[FeFe] Group A3FirmicutesSelenomonadalesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_014451418.1Fervidobacterium pennivorans[FeFe] Group A3ThermotogaeThermotogalesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 2 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_014455790.1Spirochaeta africana[FeFe] Group A3SpirochaetesSpirochaetalesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_014456422.1Spirochaeta africana[FeFe] Group A3SpirochaetesSpirochaetalesBifurcatingLabile4[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin, thioredoxin
WP_014487195.1Brachyspira intermedia[FeFe] Group A3SpirochaetesSpirochaetalesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_014553773.1Halanaerobium praevalens[FeFe] Group A3FirmicutesHalanaerobialesBifurcatingLabile4[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin, thioredoxin
WP_014559401.1Ignavibacterium album[FeFe] Group A3IgnavibacteriaeIgnavibacterialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_014758969.1Thermoanaerobacterium saccharolyticum[FeFe] Group A3FirmicutesThermoanaerobacteralesBifurcatingLabile4[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin, thioredoxin
WP_014792559.1Desulfitobacterium dehalogenans[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile4[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin, thioredoxin
WP_014795695.1Desulfitobacterium dehalogenans[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile3[FeFe]-centre, 2 x [4Fe4S] clustersNADH dehydrogenase, thioredoxin
WP_014803888.1Turneriella parva[FeFe] Group A3SpirochaetesSpirochaetalesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_014857142.1Melioribacter roseus[FeFe] Group A3IgnavibacteriaeIgnavibacterialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 2 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_014901644.1Desulfosporosinus meridiei[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_014902186.1Desulfosporosinus meridiei[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile3[FeFe]-centre, 2 x [4Fe4S] clustersNADH dehydrogenase, thioredoxin
WP_014905079.1Desulfosporosinus meridiei[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_015326098.1Halobacteroides halobius[FeFe] Group A3FirmicutesHalanaerobialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_015327873.1Halobacteroides halobius[FeFe] Group A3FirmicutesHalanaerobialesBifurcatingLabile4[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin, thioredoxin
WP_015358633.1Ruminiclostridium stercorarium[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile4[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin, thioredoxin
WP_015394438.1Clostridium saccharoperbutylacetonicum[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_015424900.1Cloacimonas acidaminovorans[FeFe] Group A3CloacimonetesUnclassifiedBifurcatingLabile1[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin

Literature

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