[FeFe] Group A3-hydrogenase

This entry was last updated at: Feb. 9, 2018, 6:46 a.m.

Properties
Group [FeFe]-hydrogenases
Subgroup [FeFe] Group A3: Bifurcating
Function Bifurcates electrons from H2 to NAD and ferredoxin. Reverse reaction can also occur resulting in reoxidation of redox carriers concomitant with fermentative H2 evolution.
Activity Electron-bifurcation
Oxygen tolerance O2-labile
Localisation Cytosolic
Distribution
Ecosystem distribution Gastrointestinal tracts, anoxic soils and sediments
Taxonomic distribution Widespread in anaerobic bacteria, including Firmicutes, Bacteroidetes, Deltaproteobacteria, and Spirochaetes
Genetic Organisation

Organisation 1: Thermotoga maritima (WP_004081677.1):


Organisation 2: Desulfovibrio fructosovorans (WP_005990603.1):

Architecture
Structures
    None
Subunits 3 or 4
Subunit description HydA (catalytic subunit)
HydB (diaphorase subunit)
HydC (thioredoxin)
In organisation 2, an additional thioredoxin (HydD) is present
Catalytic site [FeFe]-centre
FeS clusters Organisation 1: 3 [4Fe4S] clusters, 2 [2Fe2S] clusters
Organisation 2: 3 [4Fe4S] clusters, 1 [2Fe2S] cluster
Sequences in this class
NCBI AccessionOrganismHydrogenase ClassPhylumOrderActivity (Predicted)Oxygen Tolerance (Predicted)Subunits (Predicted)Metal Centres (Predicted)Accessory Subunits (Predicted)
WP_011640436.1Syntrophomonas wolfei[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_011721785.1Clostridium novyi[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_011876846.1Desulfotomaculum reducens[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile3[FeFe]-centre, 2 x [4Fe4S] clustersNADH dehydrogenase, thioredoxin
WP_011877994.1Desulfotomaculum reducens[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_011877997.1Desulfotomaculum reducens[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 2 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_011879575.1Desulfotomaculum reducens[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_011971774.1Alkaliphilus metalliredigens[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_012032547.1Pelotomaculum thermopropionicum[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_012033166.1Pelotomaculum thermopropionicum[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_012065136.1Alkaliphilus metalliredigens[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile4[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin, thioredoxin
WP_012158789.1Alkaliphilus oremlandii[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile4[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin, thioredoxin
WP_012158849.1Alkaliphilus oremlandii[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_012282074.1Heliobacterium modesticaldum[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_012301314.1Desulforudis audaxviator[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_012301316.1Desulforudis audaxviator[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile3[FeFe]-centre, 2 x [4Fe4S] clustersNADH dehydrogenase, thioredoxin
WP_012302431.1Desulforudis audaxviator[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_012302631.1Desulforudis audaxviator[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_012543947.1Coprothermobacter proteolyticus[FeFe] Group A3FirmicutesThermoanaerobacteralesBifurcatingLabile4[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin, thioredoxin
WP_012635141.1Halothermothrix orenii[FeFe] Group A3FirmicutesHalanaerobialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 2 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_012635578.1Halothermothrix orenii[FeFe] Group A3FirmicutesHalanaerobialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_012994913.1Thermoanaerobacter italicus[FeFe] Group A3FirmicutesThermoanaerobacteralesBifurcatingLabile4[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin, thioredoxin
WP_013120942.1Thermincola potens[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_013174729.1Syntrophothermus lipocalidus[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_013238135.1Clostridium ljungdahlii[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_013273987.1Lachnoclostridium saccharolyticum[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_013275360.1Thermosediminibacter oceani[FeFe] Group A3FirmicutesThermoanaerobacteralesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_013275513.1Thermosediminibacter oceani[FeFe] Group A3FirmicutesThermoanaerobacteralesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_013277223.1Acetohalobium arabaticum[FeFe] Group A3FirmicutesHalanaerobialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_013277266.1Acetohalobium arabaticum[FeFe] Group A3FirmicutesHalanaerobialesBifurcatingLabile4[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin, thioredoxin
WP_013278831.1Acetohalobium arabaticum[FeFe] Group A3FirmicutesHalanaerobialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_013290549.1Caldicellulosiruptor obsidiansis[FeFe] Group A3FirmicutesThermoanaerobacteralesBifurcatingLabile4[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin, thioredoxin
WP_013297608.1Thermoanaerobacterium thermosaccharolyticum[FeFe] Group A3FirmicutesThermoanaerobacteralesBifurcatingLabile4[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin, thioredoxin
WP_013379183.1Eubacterium limosum[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_013403316.1Caldicellulosiruptor hydrothermalis[FeFe] Group A3FirmicutesThermoanaerobacteralesBifurcatingLabile4[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin, thioredoxin
WP_013406264.1Halanaerobium hydrogeniformans[FeFe] Group A3FirmicutesHalanaerobialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_013406351.1Halanaerobium hydrogeniformans[FeFe] Group A3FirmicutesHalanaerobialesBifurcatingLabile4[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin, thioredoxin
WP_013412032.1Caldicellulosiruptor owensensis[FeFe] Group A3FirmicutesThermoanaerobacteralesBifurcatingLabile4[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin, thioredoxin
WP_013430372.1Caldicellulosiruptor kronotskyensis[FeFe] Group A3FirmicutesThermoanaerobacteralesBifurcatingLabile4[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin, thioredoxin
WP_013486450.1Ethanoligenens harbinense[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_013625341.1Syntrophobotulus glycolicus[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile3[FeFe]-centre, 2 x [4Fe4S] clustersNADH dehydrogenase, thioredoxin
WP_013625342.1Syntrophobotulus glycolicus[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile3[FeFe]-centre, 2 x [4Fe4S] clustersNADH dehydrogenase, thioredoxin
WP_013626003.1Syntrophobotulus glycolicus[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile4[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin, thioredoxin
WP_013656601.1Cellulosilyticum lentocellum[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_013657394.1Cellulosilyticum lentocellum[FeFe] Group A3FirmicutesClostridialesBifurcatingLabile4[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin, thioredoxin
WP_013756843.1Thermodesulfobium narugense[FeFe] Group A3FirmicutesThermoanaerobacteralesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_013778939.1Tepidanaerobacter acetatoxydans[FeFe] Group A3FirmicutesThermoanaerobacteralesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_013779523.1Tepidanaerobacter acetatoxydans[FeFe] Group A3FirmicutesThermoanaerobacteralesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 2 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_013780878.1Mahella australiensis[FeFe] Group A3FirmicutesThermoanaerobacteralesBifurcatingLabile4[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin, thioredoxin
WP_013781004.1Mahella australiensis[FeFe] Group A3FirmicutesThermoanaerobacteralesBifurcatingLabile3[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin
WP_013787823.1Thermoanaerobacterium xylanolyticum[FeFe] Group A3FirmicutesThermoanaerobacteralesBifurcatingLabile4[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADH dehydrogenase, thioredoxin, thioredoxin

Literature

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