[FeFe] Group A2-hydrogenase Putative
This entry was last updated at: Sept. 12, 2016, 6:43 a.m.
Properties
| Group | [FeFe]-hydrogenases |
| Subgroup | [FeFe] Group A2: Glutamate synthase-linked (putative) |
| Function | Unconfirmed role. It may couple H2 oxidation to NAD reduction, generating reductant for glutamate synthase. |
| Activity | H2-uptake? |
| Oxygen tolerance | O2-labile |
| Localisation | Cytosolic |
Distribution
| Ecosystem distribution | Gastrointestinal tracts, anoxic soils and sediments |
| Taxonomic distribution | Encoded in anaerobic bacteria, including Firmicutes, Actinobacteria, and Proteobacteria |
Genetic Organisation
e.g. Desulfovibrio vulgaris (WP_010939059.1):
Architecture
| Structures |
|
| Subunits | 2 |
| Subunit description | HydA (catalytic subunit) GltA (NADH-dependent glutamate synthase) |
| Catalytic site | [FeFe]-centre |
| FeS clusters | 3 [4Fe4S] clusters, 1 [2Fe2S] cluster |
Sequences in this class
| NCBI Accession | Organism | Hydrogenase Class | Phylum | Order | Activity (Predicted) | Oxygen Tolerance (Predicted) | Subunits (Predicted) | Metal Centres (Predicted) | Accessory Subunits (Predicted) |
|---|---|---|---|---|---|---|---|---|---|
| WP_012939287.1 | Acidaminococcus fermentans | [FeFe] Group A2 | Firmicutes | Selenomonadales | Evolving | Labile | 2 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | NADH-dependent glutamate synthase |
| WP_022741745.1 | Adlercreutzia equolifaciens | [FeFe] Group A2 | Actinobacteria | Coriobacteriales | Evolving | Labile | 2 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | NADH-dependent glutamate synthase |
| WP_006565663.1 | Anaerostipes caccae | [FeFe] Group A2 | Firmicutes | Clostridiales | Evolving | Labile | 2 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | NADH-dependent glutamate synthase |
| WP_009203940.1 | Anaerostipes hadrus | [FeFe] Group A2 | Firmicutes | Clostridiales | Evolving | Labile | 2 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | NADH-dependent glutamate synthase |
| WP_028264340.1 | Atopobium fossor | [FeFe] Group A2 | Actinobacteria | Coriobacteriales | Evolving | Labile | 2 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | NADH-dependent glutamate synthase |
| WP_021625383.1 | Atopobium sp. 810 | [FeFe] Group A2 | Actinobacteria | Coriobacteriales | Evolving | Labile | 2 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | NADH-dependent glutamate synthase |
| WP_022362192.1 | Bacteroides pectinophilus | [FeFe] Group A2 | Bacteroidetes | Bacteroidales | Evolving | Labile | 2 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | NADH-dependent glutamate synthase |
| WP_016147589.1 | Butyricicoccus pullicaecorum | [FeFe] Group A2 | Firmicutes | Clostridiales | Evolving | Labile | 2 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | NADH-dependent glutamate synthase |
| WP_015393386.1 | Clostridium saccharoperbutylacetonicum | [FeFe] Group A2 | Firmicutes | Clostridiales | Evolving | Labile | 2 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | NADH-dependent glutamate synthase |
| WP_005583237.1 | Clostridium ultunense | [FeFe] Group A2 | Firmicutes | Clostridiales | Evolving | Labile | 2 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | NADH-dependent glutamate synthase |
| WP_006235348.1 | Collinsella aerofaciens | [FeFe] Group A2 | Actinobacteria | Coriobacteriales | Evolving | Labile | 2 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | NADH-dependent glutamate synthase |
| WP_022094311.1 | Collinsella sp. CAG:166 | [FeFe] Group A2 | Actinobacteria | Coriobacteriales | Evolving | Labile | 2 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | NADH-dependent glutamate synthase |
| WP_022387742.1 | Collinsella sp. CAG:289 | [FeFe] Group A2 | Actinobacteria | Coriobacteriales | Evolving | Labile | 2 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | NADH-dependent glutamate synthase |
| WP_022349260.1 | Collinsella sp. CAG:398 | [FeFe] Group A2 | Actinobacteria | Coriobacteriales | Evolving | Labile | 2 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | NADH-dependent glutamate synthase |
| WP_013708694.1 | Coriobacterium glomerans | [FeFe] Group A2 | Actinobacteria | Coriobacteriales | Evolving | Labile | 2 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | NADH-dependent glutamate synthase |
| WP_022378933.1 | Cryptobacterium sp. CAG:338 | [FeFe] Group A2 | Actinobacteria | Coriobacteriales | Evolving | Labile | 2 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | NADH-dependent glutamate synthase |
| WP_015904515.1 | Desulfobacterium autotrophicum | [FeFe] Group A2 | Deltaproteobacteria | Desulfobacterales | Evolving | Labile | 2 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | NADH-dependent glutamate synthase |
| WP_027174791.1 | Desulfovibrio aminophilus | [FeFe] Group A2 | Deltaproteobacteria | Desulfovibrionales | Evolving | Labile | 2 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | NADH-dependent glutamate synthase |
| WP_010939059.1 | Desulfovibrio vulgaris | [FeFe] Group A2 | Deltaproteobacteria | Desulfovibrionales | Evolving | Labile | 2 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | NADH-dependent glutamate synthase |
| WP_005338995.1 | Dorea formicigenerans | [FeFe] Group A2 | Firmicutes | Clostridiales | Evolving | Labile | 2 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | NADH-dependent glutamate synthase |
| WP_028088444.1 | Dorea longicatena | [FeFe] Group A2 | Firmicutes | Clostridiales | Evolving | Labile | 2 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | NADH-dependent glutamate synthase |
| WP_021900643.1 | Eggerthella sp. CAG:1427 | [FeFe] Group A2 | Actinobacteria | Coriobacteriales | Evolving | Labile | 2 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | NADH-dependent glutamate synthase |
| WP_022363186.1 | Eggerthella sp. CAG:298 | [FeFe] Group A2 | Actinobacteria | Coriobacteriales | Evolving | Labile | 2 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | NADH-dependent glutamate synthase |
| WP_022369673.1 | Eggerthella sp. CAG:368 | [FeFe] Group A2 | Actinobacteria | Coriobacteriales | Evolving | Labile | 2 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | NADH-dependent glutamate synthase |
| WP_013978878.1 | Eggerthella sp. YY7918 | [FeFe] Group A2 | Actinobacteria | Coriobacteriales | Evolving | Labile | 2 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | NADH-dependent glutamate synthase |
| WP_013979953.1 | Eggerthella sp. YY7918 | [FeFe] Group A2 | Actinobacteria | Coriobacteriales | Evolving | Labile | 2 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | NADH-dependent glutamate synthase |
| WP_028027642.1 | Enterorhabdus mucosicola | [FeFe] Group A2 | Actinobacteria | Coriobacteriales | Evolving | Labile | 2 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | NADH-dependent glutamate synthase |
| WP_013486154.1 | Ethanoligenens harbinense | [FeFe] Group A2 | Firmicutes | Clostridiales | Evolving | Labile | 2 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | NADH-dependent glutamate synthase |
| WP_012738474.1 | Eubacterium eligens | [FeFe] Group A2 | Firmicutes | Clostridiales | Evolving | Labile | 2 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | NADH-dependent glutamate synthase |
| WP_005348317.1 | Eubacterium hallii | [FeFe] Group A2 | Firmicutes | Clostridiales | Evolving | Labile | 2 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | NADH-dependent glutamate synthase |
| WP_004065781.1 | Eubacterium plexicaudatum | [FeFe] Group A2 | Firmicutes | Clostridiales | Evolving | Labile | 2 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | NADH-dependent glutamate synthase |
| WP_021739983.1 | Eubacterium ramulus | [FeFe] Group A2 | Firmicutes | Clostridiales | Evolving | Labile | 2 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | NADH-dependent glutamate synthase |
| WP_008749893.1 | Lachnoanaerobaculum saburreum | [FeFe] Group A2 | Firmicutes | Clostridiales | Evolving | Labile | 2 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | NADH-dependent glutamate synthase |
| WP_015572916.1 | Lachnoclostridium saccharolyticum | [FeFe] Group A2 | Firmicutes | Clostridiales | Evolving | Labile | 2 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | NADH-dependent glutamate synthase |
| WP_004607263.1 | Lachnoclostridium scindens | [FeFe] Group A2 | Firmicutes | Clostridiales | Evolving | Labile | 2 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | NADH-dependent glutamate synthase |
| WP_015528722.1 | Lachnoclostridium torques | [FeFe] Group A2 | Firmicutes | Clostridiales | Evolving | Labile | 2 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | NADH-dependent glutamate synthase |
| WP_027824154.1 | Laribacter hongkongensis | [FeFe] Group A2 | Betaproteobacteria | Neisseriales | Evolving | Labile | 2 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | NADH-dependent glutamate synthase |
| WP_021726228.1 | Olsenella profusa | [FeFe] Group A2 | Actinobacteria | Coriobacteriales | Evolving | Labile | 2 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | NADH-dependent glutamate synthase |
| WP_009279121.1 | Olsenella sp. 809 | [FeFe] Group A2 | Actinobacteria | Coriobacteriales | Evolving | Labile | 2 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | NADH-dependent glutamate synthase |
| WP_013251691.1 | Olsenella uli | [FeFe] Group A2 | Actinobacteria | Coriobacteriales | Evolving | Labile | 2 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | NADH-dependent glutamate synthase |
| WP_009536327.1 | Oribacterium asaccharolyticum | [FeFe] Group A2 | Firmicutes | Clostridiales | Evolving | Labile | 2 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | NADH-dependent glutamate synthase |
| WP_007156182.1 | Oribacterium sinus | [FeFe] Group A2 | Firmicutes | Clostridiales | Evolving | Labile | 2 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | NADH-dependent glutamate synthase |
| WP_014118796.1 | Oscillibacter valericigenes | [FeFe] Group A2 | Firmicutes | Clostridiales | Evolving | Labile | 2 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | NADH-dependent glutamate synthase |
| WP_028533896.1 | Paludibacterium yongneupense | [FeFe] Group A2 | Betaproteobacteria | Neisseriales | Evolving | Labile | 2 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | NADH-dependent glutamate synthase |
| WP_011342272.1 | Pelobacter carbinolicus | [FeFe] Group A2 | Deltaproteobacteria | Desulfuromonadales | Evolving | Labile | 2 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | NADH-dependent glutamate synthase |
| WP_024622238.1 | Peptoclostridium mangenotii | [FeFe] Group A2 | Firmicutes | Clostridiales | Evolving | Labile | 2 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | NADH-dependent glutamate synthase |
| WP_008902548.1 | Peptoniphilus duerdenii | [FeFe] Group A2 | Firmicutes | Clostridiales | Evolving | Labile | 2 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | NADH-dependent glutamate synthase |
| WP_004821125.1 | Peptoniphilus indolicus | [FeFe] Group A2 | Firmicutes | Clostridiales | Evolving | Labile | 2 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | NADH-dependent glutamate synthase |
| WP_002846126.1 | Peptostreptococcus anaerobius | [FeFe] Group A2 | Firmicutes | Clostridiales | Evolving | Labile | 2 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | NADH-dependent glutamate synthase |
| WP_021130842.1 | Phaeospirillum fulvum | [FeFe] Group A2 | Alphaproteobacteria | Rhodospirillales | Evolving | Labile | 2 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | NADH-dependent glutamate synthase |
Literature
Genetics:
- Greening, C., Biswas, A., Carere, C.R., Jackson, C.J., Taylor, M.C., Stott, M.B., Cook, G.M., and Morales, S.E. (2016) Genomic and metagenomic surveys of hydrogenase distribution indicate H2 is a widely utilised energy source for microbial growth and survival. ISME J. 10: 761-777.