[NiFe] Group 4g-hydrogenase Putative

This entry was last updated at: Feb. 9, 2018, 6:24 a.m.

Properties
Group [NiFe] Group 4: Respiratory H2-evolving [NiFe] hydrogenases
Subgroup [NiFe] Group 4g: Ferredoxin-coupled
Function Unconfirmed role. May form respiratory complex that couples ferredoxinred oxidation to proton reduction. The energy generated is likely to be used to translocate sodium or protons.
Activity H2-evolution?
Oxygen tolerance O2-sensitive
Localisation Membrane-bound
Distribution
Ecosystem distribution Hydrothermal systems, other high temperature ecosystems
Taxonomic distribution Sporadically distributed in anaerobic bacteria and archaea
Genetic Organisation

e.g. Desulfosporosinus orientis (WP_014183752.1):

Architecture
Structures
    None
Subunits 8?
Subunit description MahB (hydrogenase large subunit)
MahA (hydrogenase small subunit)
MahD (NuoH-like transmembrane adaptor)
MahG (NuoL-like transmembrane adaptor)
MahC (NuoC-like transmembrane adaptor)
MahH (antiporter-like subunit) MahEF (conserved hypothetical proteins)
Catalytic site [NiFe]-centre
FeS clusters Proximal: 4Cys[4Fe4S]
Medial: Absent
Distal: Absent
Important Notes
This hydrogenase class has only been identified based on genomic predictions. It is assumed to be ferredoxin-coupled given its sequence similarity with proteins from the 4d and 4i class. However, physiological, genetic, and biochemical studies are needed to describe its properties.
Sequences in this class
NCBI AccessionOrganismHydrogenase ClassPhylumOrderActivity (Predicted)Oxygen Tolerance (Predicted)Subunits (Predicted)Metal Centres (Predicted)Accessory Subunits (Predicted)
WP_005210163.1Clostridium celatum[NiFe] Group 4gFirmicutesClostridialesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_008528233.1Halorhabdus tiamatea[NiFe] Group 4gEuryarchaeotaHalobacterialesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_008908605.1Caloramator australicus[NiFe] Group 4gFirmicutesClostridialesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_011737331.1Pelobacter propionicus[NiFe] Group 4gDeltaproteobacteriaDesulfuromonadalesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_011752735.1Thermofilum pendens[NiFe] Group 4gCrenarchaeotaThermoprotealesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_012415297.1Elusimicrobium minutum[NiFe] Group 4gElusimicrobiaElusimicrobialesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_012543586.1Coprothermobacter proteolyticus[NiFe] Group 4gFirmicutesClostridialesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_012745069.1Kosmotoga olearia[NiFe] Group 4gThermotogaeThermotogalesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_013129492.1Thermosphaera aggregans[NiFe] Group 4gCrenarchaeotaDesulfurococcalesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_013413704.1Methanothermus fervidus[NiFe] Group 4gEuryarchaeotaMethanobacterialesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_013560598.1Anaerolinea thermophila[NiFe] Group 4gChloroflexiAnaerolinealesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_013756005.1Thermodesulfobium narugense[NiFe] Group 4gFirmicutesClostridialesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_014737566.1Thermogladius cellulolyticus[NiFe] Group 4gCrenarchaeotaThermococcalesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_015284192.1Methanoregula formicica[NiFe] Group 4gEuryarchaeotaMethanomicrobialesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_015789964.1Halorhabdus utahensis[NiFe] Group 4gEuryarchaeotaHalobacterialesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_018661973.1Thermobrachium celere[NiFe] Group 4gFirmicutesClostridialesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_018963227.1Coprothermobacter platensis[NiFe] Group 4gFirmicutesClostridialesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_020962956.1Thermofilum sp. 1910b[NiFe] Group 4gCrenarchaeotaThermoprotealesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_022856172.1Thermodesulfobacterium thermophilum[NiFe] Group 4gThermodesulfobacteriaThermodesulfobacterialesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_027099538.1Clostridium paraputrificum[NiFe] Group 4gFirmicutesClostridialesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_027308223.1Caloramator sp. ALD01[NiFe] Group 4gFirmicutesClostridialesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_027624751.1Clostridium lundense[NiFe] Group 4gFirmicutesClostridialesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_028841464.1Thermodesulfobacterium hveragerdense[NiFe] Group 4gThermodesulfobacteriaThermodesulfobacterialesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein
WP_029009221.1Azospirillum halopraeferens[NiFe] Group 4gAlphaproteobacteriaRhodospirillalesEvolvingSensitive6[NiFe]-centre, 1 x [4Fe4S] clustersNuoL, NuoH, NuoC, [FeS] protein

Literature

Genetics: