[NiFe] Group 2c-hydrogenase Putative

This entry was last updated at: June 13, 2016, 11:11 a.m.

Properties
Group [NiFe] Group 2: Alternative and sensory H2-uptake [NiFe] hydrogenases
Subgroup [NiFe] Group 2c: Diguanylate cyclase-linked
Function Undetermined role. May serve as H2-sensors in regulatory cascades. Enzyme predicted to transfer H2-liberated electrons to PAS domain. Associated redox change may modulate cyclic di-GMP production and in turn various cellular processes.
Activity H2-sensing?
Oxygen tolerance O2-tolerant?
Localisation Cytosolic?
Distribution
Ecosystem distribution In low abundance in various soil and aquatic samples
Taxonomic distribution Exclusive to Proteobacteria, including methanotrophic Gammaproteobacteria and sulfate-reducing Deltaproteobacteria
Genetic Organisation

e.g. Magnetospirillum magneticum (WP_011383527.1):

Architecture
Structures
    None
Subunits 3?
Subunit description HurL (hydrogenase large subunit)
HurS (hydrogenase small subunit)
HurR (putative diguanylate cyclase and phosphodiesterase)
Catalytic site [NiFe]-centre
FeS clusters Proximal: 3Cys1Asp[4Fe4S] or 3Cys1Glu[4Fe4S]
Medial: 3Cys[3Fe4S]
Distal: 3Cys1His[4Fe4S]
Important Notes
This hydrogenase class has only been identified based on genomic predictions. The genetic organisation of the operon encoding this enzyme strongly suggests a role in cyclic di-GMP production, but this needs to be validated through physiological, biochemical, and genetic studies.
Sequences in this class
NCBI AccessionOrganismHydrogenase ClassPhylumOrderActivity (Predicted)Oxygen Tolerance (Predicted)Subunits (Predicted)Metal Centres (Predicted)Accessory Subunits (Predicted)
WP_011288617.1Dechloromonas aromatica[NiFe] Group 2cBetaproteobacteriaRhodocyclalesSensoryTolerant3[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] clusterDiguanylate cyclase / phosphodiesterase
WP_028315676.1Desulfatibacillum aliphaticivorans[NiFe] Group 2cDeltaproteobacteriaDesulfobacteralesSensoryTolerant3[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] clusterDiguanylate cyclase / phosphodiesterase
WP_015947049.1Desulfatibacillum alkenivorans[NiFe] Group 2cDeltaproteobacteriaDesulfobacteralesSensoryTolerant3[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] clusterDiguanylate cyclase / phosphodiesterase
WP_028584585.1Desulfobulbus mediterraneus[NiFe] Group 2cDeltaproteobacteriaDesulfobacteralesSensoryTolerant3[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] clusterDiguanylate cyclase / phosphodiesterase
WP_015724478.1Desulfobulbus propionicus[NiFe] Group 2cDeltaproteobacteriaDesulfobacteralesSensoryTolerant3[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] clusterDiguanylate cyclase / phosphodiesterase
WP_008870999.1Desulfonatronospira thiodismutans[NiFe] Group 2cDeltaproteobacteriaDesulfovibrionalesSensoryTolerant3[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] clusterDiguanylate cyclase / phosphodiesterase
WP_024334199.1Desulfotignum balticum[NiFe] Group 2cDeltaproteobacteriaDesulfobacteralesSensoryTolerant3[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] clusterDiguanylate cyclase / phosphodiesterase
WP_006965253.1Desulfotignum phosphitoxidans[NiFe] Group 2cDeltaproteobacteriaDesulfobacteralesSensoryTolerant3[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] clusterDiguanylate cyclase / phosphodiesterase
WP_005505415.1Grimontia hollisae[NiFe] Group 2cGammaproteobacteriaVibrionalesSensoryTolerant3[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] clusterDiguanylate cyclase / phosphodiesterase
WP_022980127.1Ideonella sp. B508-1[NiFe] Group 2cBetaproteobacteriaBurkholderialesSensoryTolerant3[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] clusterDiguanylate cyclase / phosphodiesterase
WP_012346378.1Leptothrix cholodnii[NiFe] Group 2cBetaproteobacteriaBurkholderialesSensoryTolerant3[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] clusterDiguanylate cyclase / phosphodiesterase
WP_011383527.1Magnetospirillum magneticum[NiFe] Group 2cAlphaproteobacteriaRhodospirallesSensoryTolerant3[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] clusterDiguanylate cyclase / phosphodiesterase
WP_027150633.1Methylobacter tundripaludum[NiFe] Group 2cGammaproteobacteriaMethylococcalesSensoryTolerant3[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] clusterDiguanylate cyclase / phosphodiesterase
WP_005373434.1Methylomicrobium album[NiFe] Group 2cGammaproteobacteriaMethylococcalesSensoryTolerant3[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] clusterDiguanylate cyclase / phosphodiesterase
WP_014150136.1Methylomicrobium alcaliphilum[NiFe] Group 2cGammaproteobacteriaMethylococcalesSensoryTolerant3[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] clusterDiguanylate cyclase / phosphodiesterase
WP_017841795.1Methylomicrobium buryatense[NiFe] Group 2cGammaproteobacteriaMethylococcalesSensoryTolerant3[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] clusterDiguanylate cyclase / phosphodiesterase
WP_013818241.1Methylomonas methanica[NiFe] Group 2cGammaproteobacteriaMethylococcalesSensoryTolerant3[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] clusterDiguanylate cyclase / phosphodiesterase
WP_019867142.1Methylovulum miyakonense[NiFe] Group 2cGammaproteobacteriaMethylococcalesSensoryTolerant3[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] clusterDiguanylate cyclase / phosphodiesterase
WP_007019558.1Neptuniibacter caesariensis[NiFe] Group 2cGammaproteobacteriaOceanospirillalesSensoryTolerant3[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] clusterDiguanylate cyclase / phosphodiesterase
WP_007041459.1Thiorhodococcus drewsii[NiFe] Group 2cGammaproteobacteriaChromatialesSensoryTolerant3[NiFe]-centre, 2 x [4Fe4S] clusters, 1 x [3Fe4S] clusterDiguanylate cyclase / phosphodiesterase

Literature

Genetics: