[FeFe] Group B-hydrogenase Putative
This entry was last updated at: Feb. 9, 2018, 6:17 a.m.
Properties
| Group | [FeFe]-hydrogenases |
| Subgroup | [FeFe] Group B: Colonic-type |
| Function | Unconfirmed role. Indirect evidence suggests the hydrogenase is involved in hydrogenogenic fermentation. It may couple reoxidation of ferredoxinred to the evolution of H2. |
| Activity | H2-evolution? |
| Oxygen tolerance | O2-labile |
| Localisation | Cytosolic |
Distribution
| Ecosystem distribution | Gastrointestinal tracts, anoxic soils and sediments |
| Taxonomic distribution | Widespread in colonic Bacteroides, Clostridia, and Actinobacteria |
Genetic Organisation
Organisation 1: Clostridium pasteurianum (WP_023976023.1):
Organisation 2: Clostridium carboxyidovorans (WP_007061531.1):
Architecture
| Structures |
|
| Subunits | 1 |
| Subunit description | HydM (catalytic subunit) |
| Catalytic site | [FeFe]-centre |
| FeS clusters | Organisation 1: 2 [4Fe4S] clusters Organisation 2: 3 [4Fe4S] clusters, 1 [2Fe2S] cluste |
Sequences in this class
| NCBI Accession | Organism | Hydrogenase Class | Phylum | Order | Activity (Predicted) | Oxygen Tolerance (Predicted) | Subunits (Predicted) | Metal Centres (Predicted) | Accessory Subunits (Predicted) |
|---|---|---|---|---|---|---|---|---|---|
| WP_020073353.1 | Ruminiclostridium sporosphaeroides | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_021170205.1 | Sporomusa ovata | [FeFe] Group B | Firmicutes | Selenomonadales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_021626398.1 | Atopobium sp. 810 | [FeFe] Group B | Actinobacteria | Coriobacteriales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_021724994.1 | Olsenella profusa | [FeFe] Group B | Actinobacteria | Coriobacteriales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_021803667.1 | Clostridium intestinale | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_022078722.1 | Acetobacter sp. CAG:267 | [FeFe] Group B | Alphaproteobacteria | Rhodospirillales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_022418160.1 | Acidiphilium sp. CAG:727 | [FeFe] Group B | Alphaproteobacteria | Rhodospirillales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_022743703.1 | Clostridium saccharobutylicum | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_022746733.1 | Clostridium saccharobutylicum | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_023386490.1 | Youngiibacter fragilis | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_023436998.1 | Clostridium tetani | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_023920581.1 | Ruminococcus lactaris | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_023975043.1 | Clostridium pasteurianum | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_023976023.1 | Clostridium pasteurianum | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_024621657.1 | Peptoclostridium mangenotii | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_024933083.1 | Clostridium botulinum | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_025640716.1 | Clostridium ultunense | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_026881775.1 | Clostridium akagii | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_026886567.1 | Clostridium beijerinckii | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_026887424.1 | Clostridium beijerinckii | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_026891848.1 | Lachnoclostridium aerotolerans | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_026894367.1 | Clostridiisalibacter paucivorans | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_026939128.1 | Holophaga foetida | [FeFe] Group B | Acidobacteria | Holophagales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_027090514.1 | Erysipelatoclostridium saccharogumia | [FeFe] Group B | Firmicutes | Erysipelotrichales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_027095964.1 | Ruminiclostridium viride | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_027098445.1 | Clostridium paraputrificum | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_027098927.1 | Clostridium paraputrificum | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_027207458.1 | Butyrivibrio fibrisolvens | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_027365621.1 | Desulfotomaculum alcoholivorax | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_027625395.1 | Clostridium lundense | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_027629995.1 | Ruminiclostridium cellobioparum | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_027633478.1 | Clostridium hydrogeniformans | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_027634587.1 | Clostridium hydrogeniformans | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_027637669.1 | Clostridium cadaveris | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_027642984.1 | Lachnoclostridium clostridioforme | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_028044437.1 | Stoquefichus massiliensis | [FeFe] Group B | Firmicutes | Erysipelotrichales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_028085756.1 | Dorea longicatena | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_028263961.1 | Atopobium fossor | [FeFe] Group B | Actinobacteria | Coriobacteriales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_028527755.1 | Ruminococcus gauvreauii | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_028830146.1 | Proteocatella sphenisci | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_029452114.1 | Clostridium algidicarnis | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| WP_032509674.1 | Peptoclostridium difficile | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| XP_008856018.1 | Entamoeba nuttalli | [FeFe] Group B | Amoebozoa | Amoebida | Evolving | Labile | 1 | [FeFe]-centre, 2 x [4Fe4S] clusters | None |
| AIO18483.1 | Mollicutes bacterium HR1 | [FeFe] Group B | Tenericutes | Mollicutes | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_002568888.1 | Lachnoclostridium bolteae | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_002848990.1 | Ruminococcus albus | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_003492333.1 | Clostridium sporogenes | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_003530568.1 | Ruminiclostridium leptum | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_004052440.1 | Eubacterium plexicaudatum | [FeFe] Group B | Firmicutes | Clostridiales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
| WP_004099977.1 | Acetonema longum | [FeFe] Group B | Firmicutes | Selenomonadales | Evolving | Labile | 1 | [FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] cluster | None |
Literature
Genetics:
- Calusinska, M., Happe, T., Joris, B., and Wilmotte, A. (2010) The surprising diversity of clostridial hydrogenases: a comparative genomic perspective. Microbiol. 156: 1575-1588.
- Greening, C., Biswas, A., Carere, C.R., Jackson, C.J., Taylor, M.C., Stott, M.B., Cook, G.M., and Morales, S.E. (2016) Genomic and metagenomic surveys of hydrogenase distribution indicate H2 is a widely utilised energy source for microbial growth and survival. ISME J. 10: 761-777.
- Wolf, P.G., Biswas, A., Morales, S.E., Greening, C., and Gaskins, H.R. (2016) H2 metabolism is widespread and diverse among human colonic microbes. Gut Microb. 7: 235-245.
Physiology:
- Betian, H.G., Linehan, B.A., Bryant, M.P., and Holdeman, L.V. (1977) Isolation of a cellulolytic Bacteroides sp. from human feces. Appl. Environ. Microbiol. 33: 1009-1010.
- Fischbach, M.A. and Sonnenburg, J.L. (2011) Eating for two: how metabolism establishes interspecies interactions in the gut. Cell Host Microb. 10: 336-347.