[FeFe] Group A4-hydrogenase

This entry was last updated at: June 13, 2016, 11:11 a.m.

Properties
Group FeFe]-hydrogenases
Subgroup [FeFe] Group A4: Formate dehydrogenase-linked
Function Most complexes appear to couple oxidation of formate to fermentative evolution of H2. Several complexes are multifunctional bifurcating complexes that can use the electrons from H2 or formate to simultaneously reduce NADP and ferredoxin.
Activity H2-evolution or electron-bifurcation
Oxygen tolerance O2-labile
Localisation Cytosolic
Distribution
Ecosystem distribution Gastrointestinal tracts, anoxic soils and sediments
Taxonomic distribution Present in some Firmicutes
Genetic Organisation

Organisation 1: Clostridium pasteurianum (WP_023975234.1):


Organisation 2: Clostridium autoethanogenum (WP_023162857.1):

Architecture
Structures
    None
Subunits 3 or 4
Subunit description HytA (catalytic subunit)
HytE1 (iron-sulfur subunit)
HytE2 (iron-sulfur subunit)
In organisation 2, a diaphorase (HytB), thioredoxin (HytC), and iron-sulfur protein (HytD) are also present
Catalytic site [FeFe]-centre
FeS clusters 2 [4Fe4S] clusters
Sequences in this class
NCBI AccessionOrganismHydrogenase ClassPhylumOrderActivity (Predicted)Oxygen Tolerance (Predicted)Subunits (Predicted)Metal Centres (Predicted)Accessory Subunits (Predicted)
WP_007063270.1Clostridium carboxidivorans[FeFe] Group A4FirmicutesClostridialesBifurcatingLabile6[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADP dehydrogenase, four FeS proteins
WP_011393383.1Moorella thermoacetica[FeFe] Group A4FirmicutesThermoanaerobacteralesBifurcatingLabile6[FeFe]-centre, 2 x [4Fe4S] clustersNADP dehydrogenase, four FeS proteins
WP_011989407.1Clostridium kluyveri[FeFe] Group A4FirmicutesClostridialesBifurcatingLabile6[FeFe]-centre, 2 x [4Fe4S] clustersNADP dehydrogenase, four FeS proteins
WP_013237377.1Clostridium ljungdahlii[FeFe] Group A4FirmicutesClostridialesBifurcatingLabile6[FeFe]-centre, 2 x [4Fe4S] clustersNADP dehydrogenase, four FeS proteins
WP_014355220.1Acetobacterium woodii[FeFe] Group A4FirmicutesClostridialesBifurcatingLabile6[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADP dehydrogenase, four FeS proteins
WP_015707165.1Treponema primitia[FeFe] Group A4SpirochaetesSpirochaetalesBifurcatingLabile6[FeFe]-centre, 3 x [4Fe4S] clusters, 1 x [2Fe2S] clusterNADP dehydrogenase, four FeS proteins
WP_023162857.1Clostridium autoethanogenum[FeFe] Group A4FirmicutesClostridialesBifurcatingLabile6[FeFe]-centre, 2 x [4Fe4S] clustersNADP dehydrogenase, four FeS proteins
WP_007285433.1Intestinibacter bartlettii[FeFe] Group A4FirmicutesClostridialesEvolvingLabile3[FeFe]-centre, 2 x [4Fe4S] clustersFeS protein, FeS protein
WP_007960496.1Pelosinus fermentans[FeFe] Group A4FirmicutesSelenomonadalesEvolvingLabile3[FeFe]-centre, 2 x [4Fe4S] clustersFeS protein, FeS protein
WP_010295100.1Clostridium senegalense[FeFe] Group A4FirmicutesClostridialesEvolvingLabile3[FeFe]-centre, 2 x [4Fe4S] clustersFeS protein, FeS protein
WP_010345843.1Paenibacillus peoriae[FeFe] Group A4FirmicutesBacillalesEvolvingLabile3[FeFe]-centre, 2 x [4Fe4S] clustersFeS protein, FeS protein
WP_011187724.1Desulfotalea psychrophila[FeFe] Group A4DeltaproteobacteriaDesulfobacteralesEvolvingLabile3[FeFe]-centre, 2 x [4Fe4S] clustersFeS protein, FeS protein
WP_011366599.1Desulfovibrio alaskensis[FeFe] Group A4DeltaproteobacteriaDesulfovibrionalesEvolvingLabile3[FeFe]-centre, 2 x [4Fe4S] clustersFeS protein, FeS protein
WP_011388063.1Rhodospirillum rubrum[FeFe] Group A4AlphaproteobacteriaRhodospirillalesEvolvingLabile3[FeFe]-centre, 2 x [4Fe4S] clustersFeS protein, FeS protein
WP_012031839.1Pelotomaculum thermopropionicum[FeFe] Group A4FirmicutesClostridialesEvolvingLabile3[FeFe]-centre, 2 x [4Fe4S] clustersFeS protein, FeS protein
WP_012662530.1Desulfobacterium autotrophicum[FeFe] Group A4DeltaproteobacteriaDesulfobacteralesEvolvingLabile3[FeFe]-centre, 2 x [4Fe4S] clustersFeS protein, FeS protein
WP_012765956.1Desulfovibrio salexigens[FeFe] Group A4DeltaproteobacteriaDesulfovibrionalesEvolvingLabile3[FeFe]-centre, 2 x [4Fe4S] clustersFeS protein, FeS protein
WP_013238388.1Clostridium ljungdahlii[FeFe] Group A4FirmicutesClostridialesEvolvingLabile3[FeFe]-centre, 2 x [4Fe4S] clustersFeS protein, FeS protein
WP_013275594.1Thermosediminibacter oceani[FeFe] Group A4FirmicutesThermoanaerobacteralesEvolvingLabile3[FeFe]-centre, 2 x [4Fe4S] clustersFeS protein, FeS protein
WP_014282026.1Paenibacillus terrae[FeFe] Group A4FirmicutesBacillalesEvolvingLabile3[FeFe]-centre, 2 x [4Fe4S] clustersFeS protein, FeS protein
WP_015337360.1Desulfovibrio hydrothermalis[FeFe] Group A4DeltaproteobacteriaDesulfovibrionalesEvolvingLabile3[FeFe]-centre, 2 x [4Fe4S] clustersFeS protein, FeS protein
WP_016822145.1Paenibacillus polymyxa[FeFe] Group A4FirmicutesBacillalesEvolvingLabile3[FeFe]-centre, 2 x [4Fe4S] clustersFeS protein, FeS protein
WP_018591881.1Terrisporobacter glycolicus[FeFe] Group A4FirmicutesClostridialesEvolvingLabile3[FeFe]-centre, 2 x [4Fe4S] clustersFeS protein, FeS protein
WP_018884458.1Paenibacillus massiliensis[FeFe] Group A4FirmicutesBacillalesEvolvingLabile3[FeFe]-centre, 2 x [4Fe4S] clustersFeS protein, FeS protein
WP_021432325.1Peptoclostridium bifermentans[FeFe] Group A4FirmicutesClostridialesEvolvingLabile3[FeFe]-centre, 2 x [4Fe4S] clustersFeS protein, FeS protein
WP_023975234.1Clostridium pasteurianum[FeFe] Group A4FirmicutesClostridialesEvolvingLabile3[FeFe]-centre, 2 x [4Fe4S] clustersFeS protein, FeS protein
WP_024825939.1Desulfovibrio magneticus[FeFe] Group A4DeltaproteobacteriaDesulfovibrionalesEvolvingLabile3[FeFe]-centre, 2 x [4Fe4S] clustersFeS protein, FeS protein
WP_025688954.1Paenibacillus zanthoxyli[FeFe] Group A4FirmicutesBacillalesEvolvingLabile3[FeFe]-centre, 2 x [4Fe4S] clustersFeS protein, FeS protein
WP_025700928.1Paenibacillus forsythiae[FeFe] Group A4FirmicutesBacillalesEvolvingLabile3[FeFe]-centre, 2 x [4Fe4S] clustersFeS protein, FeS protein
WP_026885750.1Clostridium beijerinckii[FeFe] Group A4FirmicutesClostridialesEvolvingLabile3[FeFe]-centre, 2 x [4Fe4S] clustersFeS protein, FeS protein
WP_027186285.1 Desulfovibrio inopinatus[FeFe] Group A4DeltaproteobacteriaDesulfovibrionalesEvolvingLabile3[FeFe]-centre, 2 x [4Fe4S] clustersFeS protein, FeS protein
WP_027721164.1Desulfovibrio zosterae[FeFe] Group A4DeltaproteobacteriaDesulfovibrionalesEvolvingLabile3[FeFe]-centre, 2 x [4Fe4S] clustersFeS protein, FeS protein
WP_028591227.1Paenibacillus panacisoli[FeFe] Group A4FirmicutesBacillalesEvolvingLabile3[FeFe]-centre, 2 x [4Fe4S] clustersFeS protein, FeS protein
WP_027186285.1Desulfovibrio inopinatus[FeFe] Group A4DeltaproteobacteriaDesulfovibrionalesEvolvingLabile3[FeFe]-centre, 2 x [4Fe4S] clustersFeS protein, FeS protein

Literature

Genetics:

Physiology: